scholarly journals Role of Sphingosine Kinase 1 and S1P Transporter Spns2 in HGF-mediated Lamellipodia Formation in Lung Endothelium

2016 ◽  
Vol 291 (53) ◽  
pp. 27187-27203 ◽  
Author(s):  
Panfeng Fu ◽  
David L. Ebenezer ◽  
Evgeny V. Berdyshev ◽  
Irina A. Bronova ◽  
Mark Shaaya ◽  
...  
Keyword(s):  
Sphingosine Kinase ◽  
Sphingosine Kinase 1 ◽  
Lung Endothelium ◽  
Lamellipodia Formation

Islet Co-Expression of CD133 and ABCB5 in Human Retinoblastoma Specimens

2021 ◽  
Author(s):  
Marco Zschoche ◽  
Sergej Skosyrski ◽  
Neele Babst ◽  
Mahdy Ranjbar ◽  
Felix Rommel ◽  
...  
Keyword(s):  
Treatment Resistance ◽  
Sphingosine Kinase ◽  
Immunohistochemical Analysis ◽  
Pcr Analysis ◽  
Sphingosine Kinase 1 ◽  
Rt Pcr ◽  
Beam Radiation ◽  
Sphingosine Kinase 2 ◽  
Human Retinoblastoma

Abstract Background The role of CD133 und ABCB5 is discussed in treatment resistance in several types of cancer. The objective of this study was to evaluate whether CD133+/ABCB5+ colocalization differs in untreated, in beam radiation treated, and in chemotherapy treated retinoblastoma specimens. Additionally, CD133, ABCB5, sphingosine kinase 1, and sphingosine kinase 2 gene expression was analyzed in WERI-RB1 (WERI RB1) and etoposide-resistant WERI RB1 subclones (WERI ETOR). Methods Active human untreated retinoblastoma specimens (n = 12), active human retinoblastoma specimens pretreated with beam radiation before enucleation (n = 8), and active human retinoblastoma specimens pretreated with chemotherapy before enucleation (n = 7) were investigated for localization and expression of CD133 and ABCB5 by immunohistochemistry. Only specimens with IIRC D, but not E, were included in this study. Furthermore, WERI RB1 and WERI ETOR cell lines were analyzed for CD133, ABCB5, sphingosine kinase 1, and sphingosine kinase 2 by the real-time polymerase chain reaction (RT-PCR). Results Immunohistochemical analysis revealed the same amount of CD133+/ABCB5+ colocalization islets in untreated and treated human retinoblastoma specimens. Quantitative RT-PCR analysis showed a statistically significant upregulation of CD133 in WERI ETOR (p = 0.002). No ABCB5 expression was detected in WERI RB1 and WERI ETOR. On the other hand, SPHK1 (p = 0.0027) and SPHK2 (p = 0.017) showed significant downregulation in WERI ETOR compared to WERI RB1. Conclusions CD133+/ABCB5+ co-localization islets were noted in untreated and treated human retinoblastoma specimens. Therefore, we assume that CD133+/ABCB5+ islets might play a role in retinoblastoma genesis, but not in retinoblastoma treatment resistance.

scholarly journals Detrimental role of sphingosine kinase 1 in kidney damage in DOCA-salt hypertensive model: evidence from knockout mice

2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Bingqing Lyu ◽  
Weili Wang ◽  
Xin-Ying Ji ◽  
Joseph K. Ritter ◽  
Ningjun Li
Keyword(s):  
Knockout Mice ◽  
Sphingosine Kinase ◽  
Kidney Damage ◽  
Sphingosine Kinase 1 ◽  
Model Evidence

scholarly journals Filamin A Links Sphingosine Kinase 1 and Sphingosine-1-Phosphate Receptor 1 at Lamellipodia To Orchestrate Cell Migration

2008 ◽  
Vol 28 (18) ◽  
pp. 5687-5697 ◽  
Author(s):  
Michael Maceyka ◽  
Sergio E. Alvarez ◽  
Sheldon Milstien ◽  
Sarah Spiegel
Keyword(s):  
Critical Role ◽  
Cell Movement ◽  
Sphingosine Kinase ◽  
Lipid Mediator ◽  
Sphingosine Kinase 1 ◽  
Filamin A ◽  
Interacting Protein ◽  
Surface Receptors ◽  
Sphingosine 1 Phosphate ◽  
Lamellipodia Formation

ABSTRACT Sphingosine kinase 1 (SphK1) catalyzes the phosphorylation of sphingosine to produce the potent lipid mediator sphingosine-1-phosphate (S1P), which plays a critical role in cell motility via its cell surface receptors. Here, we have identified filamin A (FLNa), an actin-cross-linking protein involved in cell movement, as a bona fide SphK1-interacting protein. Heregulin stimulated SphK1 activity only in FLNa-expressing A7 melanoma cells but not in FLNa-deficient cells and induced its translocation and colocalization with FLNa at lamellipodia. SphK1 was required for heregulin-induced migration, lamellipodia formation, activation of PAK1, and subsequent FLNa phosphorylation. S1P directly stimulated PAK1 kinase, suggesting that it may be a target of intracellularly generated S1P. Heregulin also induced colocalization of S1P1 (promotility S1P receptor) but not S1P2, with SphK1 and FLNa at membrane ruffles. Moreover, an S1P1 antagonist inhibited the lamellipodia formation induced by heregulin. Hence, FLNa links SphK1 and S1P1 to locally influence the dynamics of actin cytoskeletal structures by orchestrating the concerted actions of the triumvirate of SphK1, FLNa, and PAK1, each of which requires and/or regulates the actions of the others, at lamellipodia to promote cell movement.

scholarly journals Role of sphingosine kinase 1 and sphingosine‐1‐phosphate in CD40 signaling and IgE class switching

2014 ◽  
Vol 28 (10) ◽  
pp. 4347-4358 ◽  
Author(s):  
Eugene Y. Kim ◽  
Jamie L. Sturgill ◽  
Nitai C. Hait ◽  
Dorit Avni ◽  
Evelyn C. Valencia ◽  
...  
Keyword(s):  
Sphingosine Kinase ◽  
Sphingosine Kinase 1 ◽  
Class Switching ◽  
Sphingosine 1 Phosphate

scholarly journals Role of sphingosine kinase‐1 in paracrine/transcellular angiogenesis and lymphangiogenesis in vitro

2010 ◽  
Vol 24 (8) ◽  
pp. 2727-2738 ◽  
Author(s):  
Viviana Anelli ◽  
Christopher R. Gault ◽  
Ashley J. Snider ◽  
Lina M. Obeid
Keyword(s):  
Sphingosine Kinase ◽  
Sphingosine Kinase 1

scholarly journals Sphingosine Kinase 1 Is Required for Mesothelioma Cell Proliferation: Role of Histone Acetylation

PLoS ONE ◽  
2012 ◽  
Vol 7 (9) ◽  
pp. e45330 ◽  
Author(s):  
Satish Kalari ◽  
Nagabhushan Moolky ◽  
Srikanth Pendyala ◽  
Evgeny V. Berdyshev ◽  
Cleo Rolle ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Histone Acetylation ◽  
Sphingosine Kinase ◽  
Sphingosine Kinase 1 ◽  
Mesothelioma Cell

Abstract P6-06-04: The role of sphingosine kinase 1 in breast carcinogenesis

2015 ◽  
Author(s):  
Yoshiko Shimizu ◽  
Hideki Furuya ◽  
Paulette M Tamashiro ◽  
Kayoko Iino ◽  
Charles J Rosser ◽  
...  
Keyword(s):  
Sphingosine Kinase ◽  
Sphingosine Kinase 1 ◽  
Breast Carcinogenesis

Deletion of sphingosine kinase 1 ameliorates hepatic steatosis in diet-induced obese mice: Role of PPARγ

2016 ◽  
Vol 1861 (2) ◽  
pp. 138-147 ◽  
Author(s):  
Jinbiao Chen ◽  
Wei Wang ◽  
Yanfei Qi ◽  
Dominik Kaczorowski ◽  
Geoffrey W. McCaughan ◽  
...  
Keyword(s):  
Hepatic Steatosis ◽  
Sphingosine Kinase ◽  
Sphingosine Kinase 1 ◽  
Obese Mice
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