scholarly journals N-terminal Extension of Canine Glutamine Synthetase Created by Splicing Alters Its Enzymatic Property

2003 ◽  
Vol 279 (2) ◽  
pp. 1184-1190 ◽  
Author(s):  
Daesung Shin ◽  
Chankyu Park
Author(s):  
W. Kunath ◽  
E. Zeitler ◽  
M. Kessel

The features of digital recording of a continuous series (movie) of singleelectron TV frames are reported. The technique is used to investigate structural changes in negatively stained glutamine synthetase molecules (GS) during electron irradiation and, as an ultimate goal, to look for the molecules' “undamaged” structure, say, after a 1 e/Å2 dose.The TV frame of fig. la shows an image of 5 glutamine synthetase molecules exposed to 1/150 e/Å2. Every single electron is recorded as a unit signal in a 256 ×256 field. The extremely low exposure of a single TV frame as dictated by the single-electron recording device including the electron microscope requires accumulation of 150 TV frames into one frame (fig. lb) thus achieving a reasonable compromise between the conflicting aspects of exposure time per frame of 3 sec. vs. object drift of less than 1 Å, and exposure per frame of 1 e/Å2 vs. rate of structural damage.


2017 ◽  
Vol 43 (9) ◽  
pp. 1410 ◽  
Author(s):  
Xiao-Chun WANG ◽  
Hao-Ran ZHANG ◽  
Yi-Hao WEI ◽  
Xi-Ting JIA ◽  
Ming-Xin GU ◽  
...  

2013 ◽  
Vol 12 (7) ◽  
pp. 900-913 ◽  
Author(s):  
Hans-Gert Bernstein ◽  
Anne Tausch ◽  
Rebecca Wagner ◽  
Johann Steiner ◽  
Patrick Seeleke ◽  
...  

2002 ◽  
Vol 42 (supplement2) ◽  
pp. S138
Author(s):  
T. Suzuki ◽  
A. Kashiwagi ◽  
K. Mori ◽  
I. Urabe ◽  
T. Yomo

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