scholarly journals Topology of the VirB4 C Terminus in theAgrobacterium tumefaciensVirB/D4 Type IV Secretion System

2006 ◽  
Vol 281 (49) ◽  
pp. 37628-37635 ◽  
Author(s):  
Olga Draper ◽  
Rebecca Middleton ◽  
Michaeleen Doucleff ◽  
Patricia C. Zambryski
Keyword(s):  
Secretion System ◽  
Type Iv Secretion ◽  
Type Iv Secretion System ◽  
Type Iv ◽  
C Terminus

scholarly journals Stabilization of the Pertussis Toxin Secretion Apparatus by the C Terminus of PtlD

2008 ◽  
Vol 190 (21) ◽  
pp. 7285-7290 ◽  
Author(s):  
Anita Verma ◽  
Anissa M. Cheung ◽  
Drusilla L. Burns
Keyword(s):  
Amino Acids ◽  
Pertussis Toxin ◽  
Bordetella Pertussis ◽  
Secretion System ◽  
Type Iv Secretion ◽  
Type Iv Secretion System ◽  
Type Iv ◽  
C Terminus ◽  
Toxin Secretion ◽  
The Stability

ABSTRACT Pertussis toxin (PT) is secreted from Bordetella pertussis by a type IV secretion system, known as the Ptl transporter, that comprises nine different proteins, PtlA to PtlI. In this study, we found that PtlD is required for the stability of three Ptl proteins, PtlE, PtlF, and PtlH. A region limited to the C-terminal 72 amino acids of PtlD (amino acids 392 to 463) was sufficient for maintaining the stability of PtlE, PtlF, and PtlH, although this region was not sufficient to support secretion of the toxin. Further analysis demonstrated that a stretch of 10 amino acids at the C-terminal end of PtlD (amino acids 425 to 434) contributes to transporter stability.

scholarly journals Agrobacterium-Mediated T-DNA Transfer and Integration by Minimal VirD2 Consisting of the Relaxase Domain and a Type IV Secretion System Translocation Signal

2009 ◽  
Vol 22 (11) ◽  
pp. 1356-1365 ◽  
Author(s):  
Maartje van Kregten ◽  
Beatrice I. Lindhout ◽  
Paul J. J. Hooykaas ◽  
Bert J. van der Zaal
Keyword(s):  
Secretion System ◽  
Type Iv Secretion ◽  
Type Iv Secretion System ◽  
Terminal Part ◽  
Type Iv ◽  
C Terminus ◽  
Localization Signal ◽  
Translocation Domain ◽  
First Time

The VirD2 protein of Agrobacterium tumefaciens is essential for processing and transport of the T-DNA. It has at least three functional domains: a relaxase domain at the N terminus, a bipartite nuclear localization signal (NLS), and a sequence called ω at the C terminus. We confirm here that deletions of the C-terminal part of VirD2 led to lack of transfer of T-DNA but, for the first time, we report that virulence is restored when these truncations are supplemented at the C terminus by a short translocation signal from the VirF protein. The lack of virulence of C-terminal deletions suggests that the C-terminal part contains all or part of the translocation signal of VirD2. Using a novel series of mutant VirD2 proteins, the C-terminal half of VirD2 was further investigated. We demonstrate that the C-terminal 40 amino acids of VirD2, which include the NLS and ω, contain all or part of the translocation domain necessary for transport of VirD2 into plant cells, while another element is present in the middle of the protein. The finding that a type IV secretion system transport signal at the C terminus of VirD2 is necessary for virulence provides evidence for the role of VirD2 as a pilot protein driving translocation of the T-strand.

High prevalence of functional type IV secretion system for CagA protein in Japanese clinical Helicobacter pylori

2001 ◽  
Vol 120 (5) ◽  
pp. A652-A653
Author(s):  
Y HIRATA ◽  
S MAEDA ◽  
Y MITUNO ◽  
M AKANUMA ◽  
T KAWABE ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Secretion System ◽  
Type Iv Secretion ◽  
Type Iv Secretion System ◽  
Functional Type ◽  
Caga Protein ◽  
Type Iv ◽  
High Prevalence

scholarly journals 814 – Helicobacter Pylori Induces Aberrant Lrig1 Stem Cell Activity Within the Stomach in a Cag Type Iv Secretion System-Dependent Manner

2019 ◽  
Vol 156 (6) ◽  
pp. S-171-S-172 ◽  
Author(s):  
Lydia Wroblewski ◽  
Eunyoung Choi ◽  
Christine Petersen ◽  
Alberto Delgado ◽  
M. Blanca Piazuelo ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Stem Cell ◽  
Cell Activity ◽  
Secretion System ◽  
Type Iv Secretion ◽  
Type Iv Secretion System ◽  
Dependent Manner ◽  
Type Iv ◽  
Stem Cell Activity
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