scholarly journals Structural Characterization of PTX3 Disulfide Bond Network and Its Multimeric Status in Cumulus Matrix Organization

2008 ◽  
Vol 283 (15) ◽  
pp. 10147-10161 ◽  
Author(s):  
Antonio Inforzato ◽  
Vincenzo Rivieccio ◽  
Antonio P. Morreale ◽  
Antonio Bastone ◽  
Antonietta Salustri ◽  
...  
Keyword(s):  
Disulfide Bond ◽  
Structural Characterization ◽  
Matrix Organization ◽  
Bond Network

scholarly journals Disulfide bond mapping and structural characterization of spruce budworm antifreeze protein

1998 ◽  
Vol 258 (2) ◽  
pp. 445-453 ◽  
Author(s):  
Sherry Y. Gauthier ◽  
Cyril M. Kay ◽  
Brian D. Sykes ◽  
Virginia K. Walker ◽  
Peter L. Davies
Keyword(s):  
Disulfide Bond ◽  
Structural Characterization ◽  
Spruce Budworm ◽  
Antifreeze Protein

Automatic Disulfide Bond Assignment Using a1Ion Screening by Mass Spectrometry for Structural Characterization of Protein Pharmaceuticals

2012 ◽  
Vol 84 (11) ◽  
pp. 4900-4906 ◽  
Author(s):  
Sheng-Yu Huang ◽  
Yu-Ting Hsieh ◽  
Chun-Hao Chen ◽  
Chao-Chi Chen ◽  
Wang-Chou Sung ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Disulfide Bond ◽  
Structural Characterization ◽  
Protein Pharmaceuticals

scholarly journals Thermodynamics, functional and structural characterization of inosine–uridine nucleoside hydrolase from Leishmania braziliensis

RSC Advances ◽  
2017 ◽  
Vol 7 (77) ◽  
pp. 48861-48875 ◽  
Author(s):  
Pedro Ferrari Dalberto ◽  
Leonardo Kras Borges Martinelli ◽  
Jose Fernando Ruggiero Bachega ◽  
Luis Fernando Saraiva Macedo Timmers ◽  
Antonio Frederico Michel Pinto ◽  
...  
Keyword(s):  
Disulfide Bond ◽  
Structural Characterization ◽  
Leishmania Braziliensis ◽  
Nucleoside Hydrolase

Inosine–uridine nucleoside hydrolase fromLeishmania braziliensisis a nonspecific enzyme that contains a disulfide bond not needed for tetramer stabilization.

New ultrastructural findings about Borrelia burgdorferi by cryofixation, negative staining, thin sectioning and scanning techniques

1990 ◽  
Vol 48 (3) ◽  
pp. 582-583
Author(s):  
S. F. Hayes ◽  
M. D. Corwin ◽  
T. G. Schwan ◽  
D. W. Dorward ◽  
W. Burgdorfer
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Structural Characterization ◽  
Negative Staining ◽  
Low Speed ◽  
Thin Sectioning ◽  
Ultrastructural Findings

Characterization of Borrelia burgdorferi strains by means of negative staining EM has become an integral part of many studies related to the biology of the Lyme disease organism. However, relying solely upon negative staining to compare new isolates with prototype B31 or other borreliae is often unsatisfactory. To obtain more satisfactory results, we have relied upon a correlative approach encompassing a variety EM techniques, i.e., scanning for topographical features and cryotomy, negative staining and thin sectioning to provide a more complete structural characterization of B. burgdorferi.For characterization, isolates of B. burgdorferi were cultured in BSK II media from which they were removed by low speed centrifugation. The sedimented borrelia were carefully resuspended in stabilizing buffer so as to preserve their features for scanning and negative staining. Alternatively, others were prepared for conventional thin sectioning and for cryotomy using modified procedures. For thin sectioning, the fixative described by Ito, et al.

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