scholarly journals Analysis of High Throughput Protein Expression inEscherichia coli

2006 ◽  
Vol 5 (9) ◽  
pp. 1567-1580 ◽  
Author(s):  
Yair Benita ◽  
Michael J. Wise ◽  
Martin C. Lok ◽  
Ian Humphery-Smith ◽  
Ronald S. Oosting
Keyword(s):  
Protein Expression ◽  
High Throughput ◽  
Inescherichia Coli ◽  
Expression Inescherichia Coli

scholarly journals The extracellular domain of immunodeficiency virus gp41 protein: Expression inEscherichia coli, purification, and crystallization

1997 ◽  
Vol 6 (8) ◽  
pp. 1653-1660 ◽  
Author(s):  
Paul T. Wingfield ◽  
Stephen J. Stahl ◽  
Joshua Kaufman ◽  
Adam Zlotnick ◽  
C. Craig Hyde ◽  
...  
Keyword(s):  
Protein Expression ◽  
Extracellular Domain ◽  
Inescherichia Coli ◽  
Immunodeficiency Virus ◽  
Expression Inescherichia Coli

AN OPTIMIZED PROTOCOL FOR OVERPRODUCTION OF RECOMBINANT PROTEIN EXPRESSION INEscherichia coli

2014 ◽  
Vol 44 (5) ◽  
pp. 510-528 ◽  
Author(s):  
E. Bahreini ◽  
K. Aghaiypour ◽  
R. Abbasalipourkabir ◽  
M. T. Goodarzi ◽  
M. Saidijam ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Protein Expression ◽  
Recombinant Protein Expression ◽  
Inescherichia Coli ◽  
Optimized Protocol ◽  
Expression Inescherichia Coli

scholarly journals High Throughput Screen Identifies the DNMT1 (DNA Methyltransferase-1) Inhibitor, 5-Azacytidine, as a Potent Inducer of PTEN (Phosphatase and Tensin Homolog)

2020 ◽  
Vol 40 (8) ◽  
pp. 1854-1869
Author(s):  
Keith A. Strand ◽  
Sizhao Lu ◽  
Marie F. Mutryn ◽  
Linfeng Li ◽  
Qiong Zhou ◽  
...  
Keyword(s):  
Protein Expression ◽  
High Throughput ◽  
Knockout Mice ◽  
Vascular Remodeling ◽  
Dna Methyltransferase ◽  
Dna Methyltransferase 1 ◽  
Protective Effects ◽  
High Throughput Screen ◽  
Phosphatase And Tensin Homolog ◽  
Tensin Homolog

Objective: Our recent work demonstrates that PTEN (phosphatase and tensin homolog) is an important regulator of smooth muscle cell (SMC) phenotype. SMC-specific PTEN deletion promotes spontaneous vascular remodeling and PTEN loss correlates with increased atherosclerotic lesion severity in human coronary arteries. In mice, PTEN overexpression reduces plaque area and preserves SMC contractile protein expression in atherosclerosis and blunts Ang II (angiotensin II)-induced pathological vascular remodeling, suggesting that pharmacological PTEN upregulation could be a novel therapeutic approach to treat vascular disease. Approach and Results: To identify novel PTEN activators, we conducted a high-throughput screen using a fluorescence based PTEN promoter-reporter assay. After screening ≈3400 compounds, 11 hit compounds were chosen based on level of activity and mechanism of action. Following in vitro confirmation, we focused on 5-azacytidine, a DNMT1 (DNA methyltransferase-1) inhibitor, for further analysis. In addition to PTEN upregulation, 5-azacytidine treatment increased expression of genes associated with a differentiated SMC phenotype. 5-Azacytidine treatment also maintained contractile gene expression and reduced inflammatory cytokine expression after PDGF (platelet-derived growth factor) stimulation, suggesting 5-azacytidine blocks PDGF-induced SMC de-differentiation. However, these protective effects were lost in PTEN-deficient SMCs. These findings were confirmed in vivo using carotid ligation in SMC-specific PTEN knockout mice treated with 5-azacytidine. In wild type controls, 5-azacytidine reduced neointimal formation and inflammation while maintaining contractile protein expression. In contrast, 5-azacytidine was ineffective in PTEN knockout mice, indicating that the protective effects of 5-azacytidine are mediated through SMC PTEN upregulation. Conclusions: Our data indicates 5-azacytidine upregulates PTEN expression in SMCs, promoting maintenance of SMC differentiation and reducing pathological vascular remodeling in a PTEN-dependent manner.

Human Carbonic Anhydrase IV:In VitroActivation and Purification of Disulfide-Bonded Enzyme Following Expression inEscherichia coli

1997 ◽  
Vol 9 (2) ◽  
pp. 279-287 ◽  
Author(s):  
Abdul Waheed ◽  
Thanh Pham ◽  
Michelle Won ◽  
Torayuki Okuyama ◽  
William S. Sly
Keyword(s):  
Carbonic Anhydrase ◽  
Inescherichia Coli ◽  
Human Carbonic Anhydrase ◽  
Expression Inescherichia Coli
Sign in / Sign up

Export Citation Format

Share Document