Long-term Population Dynamics and in situ Physiology in Activated Sludge Systems with Enhanced Biological Phosphorus Removal Operated with and without Nitrogen Removal

2003 ◽  
Vol 26 (2) ◽  
pp. 211-227 ◽  
Author(s):  
Natuschka Lee ◽  
Per H. Nielsen ◽  
Henrik Aspegren ◽  
Mogens Henze ◽  
Karl-Heinz Schleifer ◽  
...  
Keyword(s):  
Population Dynamics ◽  
Activated Sludge ◽  
Nitrogen Removal ◽  
Phosphorus Removal ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
Biological Phosphorus ◽  
Activated Sludge Systems

Population dynamics in wastewater treatment plants with enhanced biological phosphorus removal operated with and without nitrogen removal

2002 ◽  
Vol 46 (1-2) ◽  
pp. 163-170 ◽  
Author(s):  
N. Lee ◽  
J. la Cour Jansen ◽  
H. Aspegren ◽  
M. Henze ◽  
P.H. Nielsen ◽  
...  
Keyword(s):  
Population Dynamics ◽  
Activated Sludge ◽  
Nitrogen Removal ◽  
Phosphorus Removal ◽  
The Other ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
Biological Phosphorus ◽  
P Removal

The population dynamics of activated sludge in a pilot plant with two activated sludge systems, both designed for enhanced biological phosphorus removal (EBPR), but one of them with (BNP) and the other without (BP) nitrogen removal, was monitored during a period of 2.5 years. The influent water to the pilot plant was periodically manipulated by external addition of phosphorus (P), acetate and glucose, respectively. The population dynamics and the in situ physiology were monitored by quantitative fluorescence in situ hybridization (FISH) and microautoradiography. Significant P removal was observed in both systems throughout the whole period, with significant increases of the P removal when substrates were dosed. The activated sludge in both systems contained large amounts of dense clusters of gram-negative, methylene-blue staining coccoid rods during the whole period. A large part of the clusters belonged to the β Proteobacteria, whereas the rest of the clusters belonged either to the Actinobacteria or to the α Proteobacteria. The relative abundance of Rhodocyclus-related bacteria in the activated sludge varied significantly in both systems during the whole period (from 6 to 18% in BNP, and from 4 to 28% in BP). However, no statistically significant correlation of the Rhodocyclus-related nor any of the other investigated bacterial groups to the P content of the activated sludge (correlation for all groups investigated was always < 0.5) was observed. A significant 33Pi uptake was observed by the β Proteobacteria (part of them Rhodocyclus-related, the identity of the rest unknown) and the Actinobacteria. However, not all of the Rhodocyclus-related bacteria showed 33Pi uptake. The P removal in the investigated plants is thus believed to be mediated by a mixed population consisting of a part of the Rhodocyclus-related bacteria, the Actinobacteria and other, yet unidentified bacteria.

Design of nutrient removal activated sludge systems

2003 ◽  
Vol 47 (11) ◽  
pp. 115-122 ◽  
Author(s):  
J. Manga ◽  
J. Ferrer ◽  
A. Seco ◽  
F. Garcia-Usach
Keyword(s):  
Mathematical Model ◽  
Activated Sludge ◽  
Nutrient Removal ◽  
Phosphorus Removal ◽  
Pilot Plant ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
User Friendly ◽  
Biological Phosphorus ◽  
Activated Sludge Systems

A mechanistic mathematical model for nutrient and organic matter removal was used to describe the behavior of a nitrification denitrification enhanced biological phosphorus removal (NDEBPR) system. This model was implemented in a user-friendly software DESASS (design and simulation of activated sludge systems). A 484-L pilot plant was operated to verify the model results. The pilot plant was operated for three years over three different sludge ages. The validity of the model was confirmed with data from the pilot plant. Also, the utility of DESASS as a valuable tool for designing NDEBPR systems was confirmed.

Morphology, In-Situ characterization with rRNA targetted probes and respiratory quinone profiles of enhanced biological phosphorus removal sludge

1998 ◽  
Vol 38 (8-9) ◽  
pp. 69-76 ◽  
Author(s):  
I. M. Sudiana ◽  
T. Mino ◽  
H. Satoh ◽  
T. Matsuo
Keyword(s):  
Carbon Source ◽  
Activated Sludge ◽  
Microbial Communities ◽  
Phosphorus Removal ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
In Situ Characterization ◽  
Respiratory Quinone ◽  
Biological Phosphorus

The microbial communities in activated sludge acclimated with either acetate or glucose as the major carbon source under phosphorus limited or rich conditions were investigated morphologically, phylogenetically and chemotaxonomically. The sludge with a minimized polyphosphate content was dominated by tetrad shaped bacteria, which were suspected to be ‘glycogen accumulating bacteria (GAOs) or G bacteria’ The sludge containing high polyphosphate was dominated by cluster forming coccus bacteria. Quinone analyses suggested that all the sludge tested contained various ubiquinones and menaquinones, of which the ubiquinones Q-8 and Q-10 were dominant. Analyses with rRNA targeted probes showed that beta sub class of Proteobacteria was most predominant in all sludges tested. Morphological, phylogenetic and chemotaxonomic investigation all indicated that both high and low P sludges are microbiologically diverse.

scholarly journals The occurrence of enhanced biological phosphorus removal in a 200,000 m3/day partial nitration and Anammox activated sludge process at the Changi water reclamation plant, Singapore

2016 ◽  
Vol 75 (3) ◽  
pp. 741-751 ◽  
Author(s):  
Yeshi Cao ◽  
Bee Hong Kwok ◽  
Mark C. M. van Loosdrecht ◽  
Glen T. Daigger ◽  
Hui Yi Png ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Nitrogen Removal ◽  
Phosphorus Removal ◽  
Ex Situ ◽  
Water Reclamation ◽  
Activated Sludge Process ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
Study Results ◽  
Biological Phosphorus

Mainstream partial nitritation and Anammox (PN/A) has been observed and studied in the step-feed activated sludge process at the Changi water reclamation plant (WRP), which is the largest WRP (800,000 m3/d) in Singapore. This paper presents the study results for enhanced biological phosphorus removal (EBPR) co-existing with PN/A in the activated sludge process. Both the in-situ EBPR efficiency and ex-situ activities of phosphorus release and uptake were high. The phosphorus accumulating organisms were dominant, with little presence of glycogen accumulating organisms in the activated sludge. Chemical oxygen demand (COD) mass balance illustrated that the carbon usage for EBPR was the same as that for heterotrophic denitrification, owing to autotrophic PN/A conversions. This much lower carbon demand for nitrogen removal, compared to conventional biological nitrogen removal, made effective EBPR possible. This paper demonstrated for the first time the effective EBPR co-existence with PN/A in the mainstream in a large full-scale activated sludge process, and the feasibility to accommodate EBPR into the mainstream PN/A process. It also shows EBPR can work under warm climates.

Stability and variation in sludge properties in two parallel systems for enhanced biological phosphorus removal operated with and without nitrogen removal

1996 ◽  
Vol 34 (1-2) ◽  
pp. 101-109 ◽  
Author(s):  
Natuscka M. Lee ◽  
Hans Carlsson ◽  
Henrik Aspegren ◽  
Thomas Welander ◽  
Bengt Andersson
Keyword(s):  
Activated Sludge ◽  
Nitrogen Removal ◽  
Phosphorus Removal ◽  
Winter Season ◽  
Volume Index ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
Conventional System ◽  
Sludge Properties ◽  
Biological Phosphorus

In order to introduce nitrogen removal, many conventional activated sludge systems in Sweden have been transformed into low-loaded systems during the 1990's. Occasionally these systems have been combined with enhanced biological phosphorus removal (EBPR). Increased problems of sludge loss from secondary clarifiers have aroused new interest in the properties of activated sludge. The aim of this study was to investigate the long-term variation and stability of sludge properties in two parallel EBPR systems, operating on the same wastewater. One of the systems was designed as a conventional system, and the other as a low-loaded system with nitrogen removal. The study was performed at the Sjölunda plant in Malmö during a two-year period, in a pilot plant which has been in operation since 1986. The diluted sludge volume index (DSVI) was found to be about 150 ml/g in both systems. The highest values were recorded during the winter season. The variation in the DSVI was much greater in the conventional system than in the low-loaded system. The dominating filamentous bacteria in the conventional system were Type 021N and Thiothrix, and in the low loaded system Type 0041/0675 and Type 0092. The EBPR operation induced large amounts of poly-P-containing flocs in both systems. Although it was not possible to determine to what extent the poly-P-containing flocs affected the sludge properties in this study, it was clearly demonstrated that EBPR operation may not always provide conditions which lead to superior sludge properties.

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