Morphology, In-Situ characterization with rRNA targetted probes and respiratory quinone profiles of enhanced biological phosphorus removal sludge

1998 ◽  
Vol 38 (8-9) ◽  
pp. 69-76 ◽  
Author(s):  
I. M. Sudiana ◽  
T. Mino ◽  
H. Satoh ◽  
T. Matsuo
Keyword(s):  
Carbon Source ◽  
Activated Sludge ◽  
Microbial Communities ◽  
Phosphorus Removal ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
In Situ Characterization ◽  
Respiratory Quinone ◽  
Biological Phosphorus

The microbial communities in activated sludge acclimated with either acetate or glucose as the major carbon source under phosphorus limited or rich conditions were investigated morphologically, phylogenetically and chemotaxonomically. The sludge with a minimized polyphosphate content was dominated by tetrad shaped bacteria, which were suspected to be ‘glycogen accumulating bacteria (GAOs) or G bacteria’ The sludge containing high polyphosphate was dominated by cluster forming coccus bacteria. Quinone analyses suggested that all the sludge tested contained various ubiquinones and menaquinones, of which the ubiquinones Q-8 and Q-10 were dominant. Analyses with rRNA targeted probes showed that beta sub class of Proteobacteria was most predominant in all sludges tested. Morphological, phylogenetic and chemotaxonomic investigation all indicated that both high and low P sludges are microbiologically diverse.

Metabolism of enhanced biological phosphorus removal and non-enhanced biological phosphorus removal sludge with acetate and glucose as carbon source

1999 ◽  
Vol 39 (6) ◽  
pp. 29-35 ◽  
Author(s):  
I. M. Sudiana ◽  
T. Mino ◽  
H. Satoh ◽  
K. Nakamura ◽  
T. Matsuo
Keyword(s):  
Microbial Communities ◽  
Phosphorus Removal ◽  
Reducing Power ◽  
Oxidation Potential ◽  
Bulk Solution ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
Respiratory Quinone ◽  
Significant Difference ◽  
Biological Phosphorus

Four anaerobic-aerobic sequencing batch reactors were operated with either acetate or glucose as the main carbon sources under phosphorus poor or rich conditions. Limited phosphate loading may suppress the development of polyphosphate accumulating organisms (PAOs) leading to establishment of microbial communities without polyphosphate accumulation. These microbial communities were dominated by glycogen accumulating organisms (GAOs). PAO communities were established under the P rich loading conditions. Both PAO and GAO sludges clearly show that acetate absorption was accompanied by glycolysis with subsequent accumulation of PHAs. Glucose was anaerobically converted to PHAs and glycogen. Glycolysis in PHA synthesis was indicated by the presence of a PHA component polymer, 3-hydroxyvalerate (3HV). It is suspected that synthesis of 3HV is via formation of the intermediate metabolite Propionyl-CoA which consumes reducing power and enables microorganisms to maintain intracellular reduction oxidation potential. When glucose was injected, acetate and propionate were detected in the bulk solution suggesting that fermentation may have taken place. Respiratory quinone analyses and fluorescent in situ hybridization with an RNA targeted oligonucleotide probe revealed that there was no significant difference in microbial communities among the sludges tested.

Enhancing biological phosphorus removal with glycerol

2010 ◽  
Vol 61 (7) ◽  
pp. 1837-1843 ◽  
Author(s):  
Q. Yuan ◽  
R. Sparling ◽  
P. Lagasse ◽  
Y. M. Lee ◽  
D. Taniguchi ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Carbon Source ◽  
Activated Sludge ◽  
Phosphorus Removal ◽  
Volatile Fatty Acids ◽  
Waste Activated Sludge ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
External Carbon Source ◽  
Biological Phosphorus

An enhanced biological phosphorus removal process (EBPR) was successfully operated in presence of acetate. When glycerol was substituted for acetate in the feed the EBPR process failed. Subsequently waste activated sludge (WAS) from the reactor was removed to an off-line fermenter. The same amount of glycerol was added to the WAS fermenter which led to significant volatile fatty acids (VFA) production. By supplying the system with the VFA-enriched supernatant of the fermentate, biological phosphorus removal was enhanced. It was concluded that, if glycerol was to be used as an external carbon source in EBPR, the effective approach was to ferment glycerol with waste activated sludge.

Comparison of nutrient-removing microbial communities in activated sludge from full-scale MBRs and conventional plants

2013 ◽  
Vol 68 (2) ◽  
pp. 366-371 ◽  
Author(s):  
A. M. Saunders ◽  
P. Larsen ◽  
P. H. Nielsen
Keyword(s):  
Activated Sludge ◽  
Microbial Communities ◽  
Phosphorus Removal ◽  
Membrane Bioreactors ◽  
Full Scale ◽  
Biological Phosphorus Removal ◽  
Conventional Activated Sludge ◽  
16S Rrna Pyrosequencing ◽  
Biological Phosphorus

The composition of nutrient-removing microbial communities in five full-scale membrane bioreactors (MBRs) was investigated using fluorescence in situ hybridization and 16S rRNA pyrosequencing and compared to similar analyses of conventional activated sludge (CAS) communities. The communities were highly similar but some genera that are always present in enhanced biological phosphorus removal (EBPR) (core groups) were absent in the MBRs. The overall phylogenetic similarity of the communities indicated that these differences were primarily closely related groups. More research is needed to establish the operational significance of the observed differences between MBR and CAS sludge.

Population dynamics in wastewater treatment plants with enhanced biological phosphorus removal operated with and without nitrogen removal

2002 ◽  
Vol 46 (1-2) ◽  
pp. 163-170 ◽  
Author(s):  
N. Lee ◽  
J. la Cour Jansen ◽  
H. Aspegren ◽  
M. Henze ◽  
P.H. Nielsen ◽  
...  
Keyword(s):  
Population Dynamics ◽  
Activated Sludge ◽  
Nitrogen Removal ◽  
Phosphorus Removal ◽  
The Other ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
Biological Phosphorus ◽  
P Removal

The population dynamics of activated sludge in a pilot plant with two activated sludge systems, both designed for enhanced biological phosphorus removal (EBPR), but one of them with (BNP) and the other without (BP) nitrogen removal, was monitored during a period of 2.5 years. The influent water to the pilot plant was periodically manipulated by external addition of phosphorus (P), acetate and glucose, respectively. The population dynamics and the in situ physiology were monitored by quantitative fluorescence in situ hybridization (FISH) and microautoradiography. Significant P removal was observed in both systems throughout the whole period, with significant increases of the P removal when substrates were dosed. The activated sludge in both systems contained large amounts of dense clusters of gram-negative, methylene-blue staining coccoid rods during the whole period. A large part of the clusters belonged to the β Proteobacteria, whereas the rest of the clusters belonged either to the Actinobacteria or to the α Proteobacteria. The relative abundance of Rhodocyclus-related bacteria in the activated sludge varied significantly in both systems during the whole period (from 6 to 18% in BNP, and from 4 to 28% in BP). However, no statistically significant correlation of the Rhodocyclus-related nor any of the other investigated bacterial groups to the P content of the activated sludge (correlation for all groups investigated was always < 0.5) was observed. A significant 33Pi uptake was observed by the β Proteobacteria (part of them Rhodocyclus-related, the identity of the rest unknown) and the Actinobacteria. However, not all of the Rhodocyclus-related bacteria showed 33Pi uptake. The P removal in the investigated plants is thus believed to be mediated by a mixed population consisting of a part of the Rhodocyclus-related bacteria, the Actinobacteria and other, yet unidentified bacteria.

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