scholarly journals The intake of fried virgin olive or sunflower oils differentially induces oxidative stress in rat liver microsomes

2002 ◽  
Vol 88 (1) ◽  
pp. 57-65 ◽  
Author(s):  
José L. Quiles ◽  
Jesús R. Huertas ◽  
Maurizio Battino ◽  
M. Carmen Ramírez-Tortosa ◽  
Modesta Cassinello ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Olive Oil ◽  
Rat Liver ◽  
Sunflower Oil ◽  
Virgin Olive Oil ◽  
Liver Microsomes ◽  
Rat Liver Microsomes ◽  
Frying Process ◽  
Sunflower Oils

The effects of non-fried and fried virgin olive and sunflower oils on rat liver microsomal compositional features have been investigated. In addition, plasma antioxidants (α-tocopherol and ubiquinone 9) were investigated as well as the possible oxidative modifications suffered by virgin olive and sunflower oils during the frying process. The frying process decreased the content of α-tocopherol and phenolics in the oils and increased total polar materials. Sunflower oil was affected to a greater extent than olive oil. In rats, the intake of fried oil led to higher levels of lipid peroxidation and a lower concentration of plasma antioxidants. Microsomal fatty acid and antioxidant profiles were also altered. It seems that a strong relationship exists between the loss of antioxidants and the production of toxic compounds in the oils after frying and the extent of the peroxidative events in microsomes, which were also different depending on the fat source. The highly unsaturated sunflower oil was less resistant to the oxidative stress produced by frying and led to a higher degree of lipid peroxidation in liver microsomesin vivothan virgin olive oil.

scholarly journals The intake of fried virgin olive or sunflower oils differentially induces oxidative stress in rat liver microsomes

2002 ◽  
Vol 88 (1) ◽  
pp. 57-65 ◽  
Author(s):  
Quiles J.L.* ◽  
J.R. Huertas ◽  
M. Battino ◽  
M.C. Ramírez-Tortosa ◽  
M. Cassinello ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Rat Liver ◽  
Liver Microsomes ◽  
Rat Liver Microsomes ◽  
Sunflower Oils

Olive oil protects rat liver microsomes against benzo(a)pyrene-induced oxidative damages: Anin vitro study

2008 ◽  
Author(s):  
Kasi Pandima Devi ◽  
Perumal Vijayaraman Kiruthiga ◽  
Shanmugiahthevar Karutha Pandian ◽  
Govindaraju Archunan ◽  
Solayan Arun
Keyword(s):  
Olive Oil ◽  
Rat Liver ◽  
Liver Microsomes ◽  
Vitro Study ◽  
Rat Liver Microsomes ◽  
Oxidative Damages

Lipid Peroxidation and Cherniluminescence during Naproxen Metabolism in Rat Liver Microsomes

1994 ◽  
Vol 13 (12) ◽  
pp. 831-838 ◽  
Author(s):  
Hiroyuki Yokoyama ◽  
Toshiharu Horie ◽  
Shoji Awazu
Keyword(s):  
Lipid Peroxidation ◽  
Singlet Oxygen ◽  
Rat Liver ◽  
Liver Microsomes ◽  
Thiobarbituric Acid ◽  
Rat Liver Microsomes ◽  
Oxygen Species ◽  
Thiobarbituric Acid Reactive Substances ◽  
Microsomal Suspension ◽  
Weight Protein

1 Rat liver microsomal suspension containing NADPH and MgCl2 was incubated at 37°C with naproxen, a non-steroidal anti-inflammatory drug. Thiobarbituric acid reactive substances (TBA-RS), high molecular weight protein aggregates and fluorescent substances were formed in the microsomal suspension. 2 Chemiluminescence was produced from the microsomal suspension. This chemiluminescence production was well correlated to the TBA-RS formation, indicating that the chemiluminescence production was closely associated with the lipid peroxidation. 3 The addition of SKF-525A to the microsomal suspension inhibited the production of TBA-RS, chemiluminescence and 6-demethylnaproxen (6-DMN), the oxidative product of naproxen. Further, the antioxidant, α-tocopherol and singlet oxygen quenchers like histidine, dimethylfuran and 1,4-diazabicyclo[2,2,2]octane strikingly inhibited the productions of chemiluminescence and TBA-RS. 4 Neither naproxen nor 6-DMN caused lipid peroxidation in the absence of NADPH. Thus, lipid peroxidation and chemiluminescence during the oxidation of naproxen in liver microsomes was suggested to be provoked by reactive oxygen species and an origin of chemiluminescence was shown to be singlet oxygen.

scholarly journals The stimulatory effects of asbestos on NADPH-dependent lipid peroxidation in rat liver microsomes

1987 ◽  
Vol 241 (2) ◽  
pp. 561-565 ◽  
Author(s):  
M Fontecave ◽  
D Mansuy ◽  
M Jaouen ◽  
H Pezerat
Keyword(s):  
Lipid Peroxidation ◽  
Rat Liver ◽  
Active Sites ◽  
Oxygen Radicals ◽  
Liver Microsomes ◽  
Rat Liver Microsomes ◽  
Iron Ions ◽  
Ferrous Ions ◽  
Solid Iron ◽  
Ferric Oxides

Lipid peroxidation in rat liver microsomes induced by asbestos fibres, crocidolite and chrysotile, is greatly increased in the presence of NADPH, leading to malondialdehyde levels comparable with those induced by CCl4, a very strong inducer of lipid peroxidation. This synergic effect only occurs during the first minutes and could be explained by an increase or a regeneration of the ferrous active sites of asbestos by NADPH, which in turn could rapidly be prevented by the adsorption of microsomal proteins on the surface of the fibres. It is not inhibited by superoxide dismutase, catalase and mannitol, indicating that oxygen radicals are not involved in the reaction. It is also not inhibited by desferrioxamine, indicating that it is not due to a release of free iron ions in solution from the fibres. Lipid peroxidation in NADPH-supplemented microsomes is also greatly increased upon addition of magnetite. This could be linked to the presence of ferrous ions in this solid iron oxide, since the ferric oxides haematite and goethite are completely inactive.

Cromoglycate markedly activates ascorbate-ADP initiated lipid peroxidation in washed rat liver microsomes

1993 ◽  
Vol 21 (4) ◽  
pp. 418S-418S
Author(s):  
TASHA GANDAMIHARDJA ◽  
TERENCE HALLINAN ◽  
JAYESH GOR ◽  
CATHERINE RICE-EVANS
Keyword(s):  
Lipid Peroxidation ◽  
Rat Liver ◽  
Liver Microsomes ◽  
Rat Liver Microsomes
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