thiobarbituric acid reactive substances
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2021 ◽  
Vol 7 (12) ◽  
pp. 116001-116018
Author(s):  
Elisângela Miranda de Jesus Lisboa ◽  
Lucinéia Reuse Albiero ◽  
Nadila Melchiors ◽  
Wesley Sandro de Paula Borges ◽  
Valfran da Silva Lima ◽  
...  

Ethnobotanical surveys have revealed the use of jatobá for the treatment of several diseases. This study determined the effect of plant extracts on the development of Ehrlich carcinoma. Male Swiss mice (n=6) were subcutaneously inoculated with 106 tumor cells and intragastrically administered ethanol (2 mg·mL-1, 5 mg·mL-1, or 10 mg·mL-1) or aqueous extracts of jatobá seed or bark for 90 days. Tumor development did not significantly differ between the groups studied; however, animals treated with the aqueous extract of the seed (2.205 mg·mL-1) had a reduction in tumor size compared to those treated with the aqueous extract of the bark (1.7 mg·mL-1). The treatment was not found to influence the survival of the animals studied. A new group of animals (n=7), with or without the tumor, received the aqueous extract of jatobá seed for 7, 14, and 30 days to evaluate oxidative stress. The extract reduced the thiobarbituric acid reactive substances levels at 7 days in the liver and kidneys, and 14 days in brain and renal tissue. Protein carbonylation levels were also reduced at 7 days in the liver and brain tissue and 14 days in the liver. The reduced glutathione levels diminished in animals treated for 7 and 14 days. We conclude that treatment with the aqueous extract of the jatobá seed presents promising activity in the reduction of oxidative stress.


Processes ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 31
Author(s):  
Yu-Wen Hsu ◽  
Ya-Yu Chen ◽  
Chia-Fang Tsai

The protective effects of chlorogenic acid (CGA) against liver injury were evaluated by its reduction in carbon tetrachloride (CCl4)-induced hepatic damage in ICR mice. The animals were orally given CGA (60, 100, and 200 mg/kg, respectively) or silymairn (200 mg/kg) daily with 0.3% CCl4 administration (3 mL/kg, dissolved in olive oil) after medicament treatment on the 7th day. Compared with the normal group, CCl4 caused severe impairment in liver according to the evidence of significant reduction in the level of total albumin and expansion (p < 0.05) of the activities in aspartate aminotransferase (AST) and alanine aminotransferase (ALT), cholesterol, triglyceride (TG), and total albumin in serum, decreased the level of glutathione (GSH), and diminished the activities of catalase, superoxide dismutase (SOD), glutathione reductase (GSH-Rd), and glutathione peroxidase (GSH-Px) in liver while increasing the level of hepatic thiobarbituric acid-reactive substances (TBARS). However, oral administration of CGA or silymarin could significantly (p < 0.05) decrease the serum levels of AST, ALT, cholesterol, TG, and total albumin and elevated the serum total albumin and the activities of GSH, catalase, SOD, GSH-Rd, and GSH-Px while leading to decline the TBARS in liver compared with CCl4-intoxicated group. Moreover, histopathology displayed that CGA decreased the formation of lesions in liver resulted from CCl4. The outcomes indicate that CGA shows the efficiency hepatoprotective consequences for CCl4-incited liver injuries in mice by the elevation of the activities of antioxidant enzymes and hindrance of lipid peroxidation.


Pharmacology ◽  
2021 ◽  
pp. 1-6
Author(s):  
Masashi Tawa ◽  
Takashi Shimosato ◽  
Keisuke Nakagawa ◽  
Tomio Okamura ◽  
Mamoru Ohkita

Soluble guanylate cyclase (sGC) plays an important role in nitric oxide (NO)-mediated regulation of vascular tone; however, NO bioavailability is often reduced in diseased blood vessels. Accumulating evidence suggests that a shift of sGC from the NO-sensitive form to the NO-insensitive form could be an underlying cause contributing to this reduction. Herein, we investigated the impact of renovascular hypertension on NO-sensitive and NO-insensitive sGC-mediated relaxation in rat aortas. Renovascular hypertension was induced by partially clipping the left renal artery (2-kidneys, 1-clip; 2K1C) for 10 weeks. Systolic, diastolic, and mean arterial pressures were significantly increased in the 2K1C group when compared with the sham group. In addition, plasma thiobarbituric acid reactive substances and aortic superoxide generation were significantly enhanced in the 2K1C group when compared with those in the sham group. The vasorelaxant response of isolated aortas to the sGC stimulator BAY 41-2272 (NO-sensitive sGC agonist) was comparable between the sham and 2K1C groups. Likewise, the sGC activator BAY 60-2770 (NO-insensitive sGC agonist)-induced relaxation did not differ between the sham and 2K1C groups. In addition, the cGMP mimetic 8-Br-cGMP (protein kinase G agonist) induced similar relaxation in both groups. Furthermore, there were no differences in BAY 41-2272-stimulated and BAY 60-2770-stimulated cGMP generation between the groups. These findings suggest that the balance between NO-sensitive and NO-insensitive forms of sGC is maintained during renovascular hypertension. Therefore, sGC might not be responsible for the reduced NO bioavailability observed during renovascular hypertension.


2021 ◽  
pp. 908-913
Author(s):  
Eman, G. A. Allafi ◽  
Om-alsaad E. I. Omar ◽  
Fayrouz. A. Khaled

Tramadol manhandle straightforwardly impacts the discharge of luteinizing hormone and follicular stimulating g hormone from the front pituitary organ that diminishes the common discharge of luteinizing hormone in a pulsatile way, resulting in a negative impact on male testiclesdue to lower levels of testosterone hormone. Animals were orally given 40 mg/kg B.W. doses of tramadol. The tried measurements were given to rabbits each other day for 6 weeks. Tramadol significantly decreased body weight (BW), weight of brain, testes, testosterone, estradiol, follicle stimulating hormone (FSH), luteinizing hormone (LH), triiodothyronine (T3) and thyroxin hormone (T4). While, it caused significant increase in thiobarbituric acid-reactive substances (TBARS) concentrations in plasma, testes and brain. Keywords: Tramadol; Hormone; TBARS; Rabbits


2021 ◽  
Vol 8 (1) ◽  
pp. 45-53
Author(s):  
Ľudmila Sirotová ◽  
Marcela Matulová

Electrochemical DNA/screen-printed electrode biosensor (DNA/SPE biosensor) was tested for the detection of alterations in DNA formed as a consequence of the reaction between DNA and oxidative products of fatty acids. Interaction of DNA with a mixture of products generated during the oxidation of linoleic and oleic acids manifested DNA damage depending on a tested fatty acid and the presence of hydroperoxides and thiobarbituric acid reactive substances (TBARS) determined after the oxidation of fatty acids. A bigger extent of the DNA damage was registered in the case of the interaction with oxidized linoleic acid with the high content of TBARS. The results achieved suggest the possible application of DNA/SPE biosensor in the detection of an interaction between DNA and products of fatty acid oxidation.


2021 ◽  
Vol 24 (2) ◽  
pp. 67-75
Author(s):  
Mihaela Saracila ◽  
Arabela Elena Untea ◽  
Tatiana Dumitra Panaite

Abstract The purpose of the study was to increase the nutritional quality of pork, and to investigate the effect of the synbiotic on the oxidative stability of lipid. A 4 weeks experiment was performed on 8 weaned piglets (age 47 days, TOPIGS), divided into 2 groups (C, E). The control group (C) received a conventional diet, while the experimental group (E) included a diet supplemented with organic Zn and 10 g/kg synbiotic mixture. The productive parameters were evaluated. After slaughtered, muscle (tenderloin, shoulder, loin, ham and belly) and organs samples (liver, spleen and kidney) were collected. The muscle and organs samples were analysed regarding the proximate chemical analysis (dry matter, crude protein, crude fat, and ash). The antioxidant capacity and thiobarbituric acid reactive substances (TBARS) were performed on shoulder and ham samples at first day of the trial, 4 and 7 days after refrigeration. The crude fat determined in the loin samples, recorded a significant increase in the E group compared to the C group. The ash was higher in the shoulder samples from C group than in E group. At 7 days of refrigeration, the MDA concentration was significantly lower in the shoulder and ham samples from E group than those from C group. The conclusion was that the diet supplemented with organic Zn and 10 g/kg synbiotic mixture contributed to the delay of the lipid oxidation process of the shoulder and ham samples during the refrigeration period.


2021 ◽  
pp. 096032712110532
Author(s):  
Manigandan Nagarajan ◽  
Boobalan Raja ◽  
Jeganathan Manivannan

Due to the prevalence of hypertension (one of the major risk factors of CVD) in the population, it is necessary to explore the adverse effects of daily tolerable and “safe” dose of bisphenol A (BPA) under hypertensive conditions. The current study exposed the Nω-nitro-l-arginine methyl ester (L-NAME, 40 mg/kg b.w/day) induced hypertensive Wistar rats to BPA (50 μg/kg b.w/day) by oral administration along with appropriate controls for 30 days period. The results illustrate that a ‘safe’ dose of BPA does not influence the systolic blood pressure (SBP) and levels of circulatory biomarkers of tissue damage. On the other hand, BPA exposure significantly ( p < 0.05) elevates the thiobarbituric acid reactive substances (TBARS) content in plasma and tissues (heart, aorta, liver and kidney) in hypertensive rats when compared with respective control (BPA alone exposed) rats. Similarly, a significant modulation of ROS generation in RBC, plasma nitric oxide (NO) level and angiotensin-converting enzyme (ACE) activity was observed only under hypertensive milieu. In conclusion, the observed adverse effects during ‘safe’ dose of BPA exposure are specific to the hypertensive condition. Therefore, a precise investigation to explore the effects of BPA exposure in vulnerable hypertensive populations is highly suggested.


2021 ◽  
Vol 3 (6) ◽  
pp. 58-63
Author(s):  
V. L. de Souza ◽  
E. T. G. Stutz ◽  
M. E. De S. F. Pehrson ◽  
G. C. Coelho ◽  
C. C. Netto ◽  
...  

Healthy mice were submitted or not to daily chronic swimming and/or daily yerba mate (YM) infusion intake and had their bloods analyzed after four weeks. We hypothesized that daily intake of YM may act differently on lipid mobilization and, on the creatine, lactate and irisin levels in sedentary mice and those submitted to chronic exercise. The groups were (n=48): YM-non-treated control (NTC), YM-non-treated swimming control (NSC), non-roasted YM (NRY), roasted YM (RY), swimming plus non-roasted YM (SNRY), swimming plus roasted YM (SRY). No significant (P>0.05) difference was observed concerning creatine phosphoskinase (CPK) value. Lactate level decreased significantly (P<0.05) for SNRY and SRY, suggesting accelerated lipid mobilization and glycogen sparing potential. There was significant (P<0.05) body weight loss in all, except for NTC. Higher weight loss in NRY, RY, SNR and SRY indicated greater lipid mobilization. Thiobarbituric acid reactive substances (TBARS) and irisin had significantly (P<0.05) higher values for RY. This is first report that deals with the effect of YM consumption on serum irisin level. Major amounts of caffeic acid derivatives, slightly increased caffeine and less amount of rutin may be responsible by accelerated lipoperoxidation in sedentary healthy mices chronically treated with roasted YM. Chronically consumed YM plus swimming aid in healthy weight loss. Sedentary lifestyle plus roasted YM chronic consumption may cause accelerated lipid mobilization and increased TBARS production via irisin overexpression leading to an increase in lipoperoxidation.


Foods ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2825
Author(s):  
Bertoka Fajar Surya Perwira Negara ◽  
Hee-Jin Gong ◽  
Mi-Jeong Lee ◽  
Jae-Suk Choi

In Korea, the web-foot octopus (Amphioctopus sp.) is commonly consumed as jjukkumi bokkeum, a spicy stir-fried octopus dish. Using steaming and smoking methods, we made jjukkumi bokkeum home meal replacement (HMR) products. The response surface methodology (RSM) was employed to optimize the steam and smoke processes. Quick freezing was applied to freeze the test product at −35 °C. Then, the physicochemical, biological, nutritional characteristics, and shelf-life of the test HMR products were evaluated. The optimal conditions for steaming and smoking were 95 °C for 2 min and 70 °C for 11 min, respectively. The pH, volatile basic nitrogen content, and thiobarbituric acid-reactive substances content decreased after steaming and smoking, indicating that these processes maintained these parameters well. Sensory evaluation revealed that there were no changes in these characteristics after freezing and reheating. Further, the test HMR products contained the daily nutritional requirements of macro and micronutrients, as well as amino acids and fatty acids. The shelf-life of the HMR products was estimated to be 15 months. The findings of this study indicate that the application of steam and smoke processes to produce a jjukkumi bokkeum HMR product results in a high-quality product with a long shelf-life.


2021 ◽  
Vol 26 ◽  
pp. 1-6
Author(s):  
Julia Casagrande Bitencourt ◽  
Eduarda Valim Pereira ◽  
Ian Rabelo Gabriel ◽  
Joni Marcio de Farias

O estudo tem como objetivo avaliar a contribuição do treinamento resistido em marcadores de estresse oxidativo e força de pessoas vivendo com HIV. Trata-se de um ensaio clínico não randomizado, com delineamento de série temporal descontinua, e amostragem por voluntários pertencentes ao Programa de Atenção Municipal às DST/HIV/AIDS (PAMDHA) com tratamento de Terapia Antirretroviral de Alta Atividade. Foram realizadas avaliações de composição corporal, aptidão física, dano oxidativo e enzimas antioxidantes. Nas variáveis antropométricas entre homens foi encontrada diferenças entre os grupos nos valores de circunferência abdominal (pré = 93,95 ± 8,21; pós = 92,25 ± 7,46) e percentual de gordura (pré = 88,5 ± 6,35; pós = 18,57 ± 7,74). Os níveis de força indicaram melhora em ambos os grupos (p < 0,05). As análises de grupamentos Carbonila, da atividade da enzima antioxidante Catalase, Superóxido Dismutase (SOD), substâncias reativas ao ácido tiobarbitúrico - Thiobarbituric acid reactive substances (TBARS) e os níveis de TNF-α no soro de indivíduos HIV+, não apresentaram diferenças significativas (p < 0,05). Os resultados demonstraram que o treinamento resistido de intensidade moderada pode ser utilizado como agente terapêutico adjunto ao uso da TARV para a manutenção e melhora da saúde de pessoas com HIV.


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