Detection of inducible nitric oxide synthase inSchistosoma japonicumandS. mansoni

AbstractSchistosoma japonicumandS. mansoniwere tested for reactivity with an anti-inducible nitric oxide (iNOS) antibody and the distribution of iNOS was studied by immunofluorescent tests in different stages of the parasites. Reactivity was associated with the tegument in both larval schistosomes (sporocysts and cercariae) and eggs. With adult worms, the majority of the immunofluorescence was predominantly subtegumental inS. japonicumand parenchymal inS. mansoni. Fluorescence was also observed in host tissues (snails and mouse liver). In Western blots, the enzyme ofS. japonicumhad an apparent molecular weight of about 210 kDa. The possible role of worm and host iNOS in the parasite–host interrelation remains to be clarified.

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Vasculoprotective Role of Inducible Nitric Oxide Synthase at Inflammatory Coronary Lesions Induced by Chronic Treatment With Interleukin-1β in Pigs in Vivo

Circulation ◽

10.1161/01.cir.96.9.3104 ◽

1997 ◽

Vol 96 (9) ◽

pp. 3104-3111 ◽

Cited By ~ 27

Author(s):

Yoshihiro Fukumoto ◽

Hiroaki Shimokawa ◽

Toshiyuki Kozai ◽

Toshiaki Kadokami ◽

Kouichi Kuwata ◽

...

Keyword(s):

Nitric Oxide ◽

Nitric Oxide Synthase ◽

Inducible Nitric Oxide Synthase ◽

Chronic Treatment ◽

Interleukin 1Β ◽

Coronary Lesions ◽

Oxide Synthase ◽

Inducible Nitric Oxide

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Role of Inducible Nitric Oxide Synthase in Endotoxin-induced Acute Lung Injury

American Journal of Respiratory and Critical Care Medicine ◽

10.1164/ajrccm.158.6.9802100 ◽

1998 ◽

Vol 158 (6) ◽

pp. 1883-1889 ◽

Cited By ~ 226

Author(s):

ARNOLD S. KRISTOF ◽

PETER GOLDBERG ◽

VICTOR LAUBACH ◽

SABAH N. A. HUSSAIN

Keyword(s):

Nitric Oxide ◽

Acute Lung Injury ◽

Nitric Oxide Synthase ◽

Lung Injury ◽

Inducible Nitric Oxide Synthase ◽

Oxide Synthase ◽

Inducible Nitric Oxide

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Geldanamycin inhibits hemorrhage-induced increases in caspase-3 activity: role of inducible nitric oxide synthase

Journal of Applied Physiology ◽

10.1152/japplphysiol.00100.2007 ◽

2007 ◽

Vol 103 (3) ◽

pp. 1045-1055 ◽

Cited By ~ 13

Author(s):

Juliann G. Kiang ◽

Phillip D. Bowman ◽

Xinyue Lu ◽

Yansong Li ◽

Brian W. Wu ◽

...

Keyword(s):

Nitric Oxide ◽

Nitric Oxide Synthase ◽

Inducible Nitric Oxide Synthase ◽

Caspase 3 ◽

Gene Transfection ◽

Organ Injury ◽

Molecular Events ◽

Oxide Synthase ◽

Inducible Nitric Oxide

Hemorrhage has been shown to increase inducible nitric oxide synthase (iNOS) and deplete ATP levels in tissues and geldanamycin limits both processes. Moreover, it is evident that inhibition of iNOS reduces caspase-3 and increases survival. Thus we sought to identify the molecular events responsible for the beneficial effect of geldanamycin. Hemorrhage in mice significantly increased caspase-3 activity and protein while treatment with geldanamycin significantly limited these increases. Similarly, geldanamycin inhibited increases in proteins forming the apoptosome (a complex of caspase-9, cytochrome c, and Apaf-1). Modulation of the expression of iNOS by iNOS gene transfection or siRNA treatment demonstrated that the level of iNOS correlates with caspase-3 activity. Our data indicate that geldanamycin limits caspase-3 expression and protects from organ injury by suppressing iNOS expression and apoptosome formation. Geldanamycin, therefore, may prove useful as an adjuvant in fluids used to treat patients suffering blood loss.

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