Ex situ conservation of Aconitum heterophyllum Wall.—an endangered medicinal plant of the Himalaya through mass propagation and its effect on growth and alkaloid content

2005 ◽  
Vol 3 (2) ◽  
pp. 127-135 ◽  
Author(s):  
Subedar Pandey ◽  
Rekha Kushwaha ◽  
Om Prakash ◽  
Amita Bhattacharya ◽  
P.S. Ahuja

The endangered alpine plant Aconitum heterophyllum was conserved under ex situ conditions (greenhouse and open) of Palampur by overcoming seed dormancy and plant establishment through hot water treatment (40–60 °C for 30–120 s) of seeds. High seed germination (88 and 76%) was obtained when seeds were treated with hot water at 45 and 50 °C for 90 s. Correlation studies also revealed that treatment enhanced vegetative growth and reproductive yield of the plants. The total alkaloid content of the roots and rhizomes of the plants growing under ex situ conditions was lower than the ones collected from the natural habitat in the 1-year-old plants. However, in the 2-year-old plants, it was almost at par with the ones collected from nature. The life cycle of the plants growing under ex situ conditions also did not vary largely from the plants growing under in situ conditions. Therefore, the present study indicates the successful adaptation of A. heterophyllum plants in conditions other than their natural habitat and hence its potential for sustainable commercialization.

2019 ◽  
Vol 19 (2) ◽  
pp. 161
Author(s):  
Imawan Wahyu Hidayat ◽  
Neneng Ine Kurnita ◽  
Dimas Ardiyanto

Abstract: The threats to the plant biodiversity become more advance along with rapid degradation of the natural habitat. Plants preservation needs accompaniment between in-situ and ex-situ conservation altogether. The ex-situ plant conservation plays more important roles in order to help conserve threatened plant species. Cibodas Botanic Garden (CBG) is a government institution which has the main assignment on ex-situ plant conservation, especially tropical mountainous plants. In the last decade, CBG has conducted exploration and plants collection in order to retrieve them from the destructive pressures in their habitat. This study aimed to asses the CBG’s contribution to ex-situ plant conservation through the addition number of planted plants and to describe the important value to conservation. The study conducted through a quantitative descriptive method, based on data inventory of the newly planted plant in the garden from 2008 to 2018. This also assessed the increasing collection of each year and descriptively explaining the background data, species variety, and their conservation status. The results described that the additional number was 473 specimens and the average increase was 43 specimens per-year. These originated from exploration and plants collection 248 specimens, donation 217 specimens, self-propagation seven specimens, and one specimen from seeds exchange. The most planted was Nepenthaceae with 84 specimens, secondly Phillantaceae with 21 specimens, and Lauraceae 15 specimens. There were 116 species (152 specimens) which have conservation status. These expected to be a comprehensive description of the CBG’s plant collection and meaningful for the conservation efforts in general.Keywords: ex-situ plant conservation; garden collection; Cibodas Botanic Garden (CBG)


Agronomy ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 638
Author(s):  
Marcelo B. Medeiros ◽  
José F. M. Valls ◽  
Aluana G. Abreu ◽  
Gustavo Heiden ◽  
Suelma Ribeiro-Silva ◽  
...  

This study presents the status of ex situ and in situ conservation for the crop wild relatives of rice, potato, sweet potato, and finger millet in Brazil, and the subsequent germplasm collection expeditions. This research is part of a global initiative entitled “Adapting Agriculture to Climate Change: Collecting, Protecting, and Preparing Crop Wild Relatives” supported by the Global Crop Diversity Trust. Species of the primary, secondary, and tertiary gene pools with occurrences reported in Brazil were included: Oryza alta Swallen, O. grandiglumis (Döll) Prod., O. latifolia Desv., O. glumaepatula Steud., Eleusine tristachya (Lam.) Lam., E. indica (L.) Gaertn., Solanum commersonii Dunal, S. chacoense Bitter, Ipomoea grandifolia (Dammer) O’Donell, I. ramosissima (Poir.) Choisy, I. tiliacea (Willd.) Choisy, I. triloba L., and I. cynanchifolia Meisn. The status of the ex situ and in situ conservation of each taxon was assessed using the gap analysis methodology, and the results were used to plan 16 germplasm collection expeditions. Seeds of the collected material were evaluated for viability, and the protocols for seed germination and cryopreservation were tested. The final conservation score, resulting from the gap analysis and including the average of the ex situ and in situ scores, resulted in a classification of medium priority of conservation for all the species, with the exception of I. grandifolia (high priority). The total accessions collected (174) almost doubled the total accessions of these crop wild relatives incorporated in Embrapa’s ex situ conservation system prior to 2015. In addition, accessions for practically absent species were collected for the ex situ conservation system, such as Ipomoea species, Eleusine indica, and Solanum chacoense. The methods used for dormancy breaking and low temperature conservation for the Oryza, Eleusine, and Ipomoea species were promising for the incorporation of accessions in the respective gene banks. The results show the importance of efforts to collect and conserve ex situ crop wild relatives in Brazil based on previous gap analysis. The complementarity with the in situ strategy also appears to be very promising in the country.


Crop Science ◽  
2006 ◽  
Vol 46 (1) ◽  
pp. 428-436 ◽  
Author(s):  
Elizabeth B. Rice ◽  
Margaret E. Smith ◽  
Sharon E. Mitchell ◽  
Stephen Kresovich

Author(s):  
Roland Bourdeix ◽  
Steve Adkins ◽  
Vincent Johnson ◽  
Lalith Perera ◽  
Sisunandar

Genetika ◽  
2004 ◽  
Vol 36 (3) ◽  
pp. 221-227
Author(s):  
Jelena Aleksic ◽  
Sasa Orlovic

Principles of the conservation of genetic resources of elms (Ulmus spp) do not differ fundamentally from the general principles accepted for the conservation of genetic resources of other common Noble Hardwoods. Efficient conservation can best be achieved through appropriate combination of in situ and ex situ methods, which have distinct advantages. Besides that, ex situ conservation is employed when emergency measures are needed for rare endangered populations and when populations are too small to be managed in situ (e.g. risks of genetic drift and inbreeding). The aim of our research is ex situ conservation of genetic resources of field elm {Ulmus minor Mill) and European white elm (Ulmus laevis Pall) through establishment of field genebanks. Sampling was conducted in one population of field elm and one population of white elm. Plant material (buds) from 8 trees of field elm and 10 trees of white elm was used for in vitro production of clones. Obtained clones will be used for establishment of field genebanks on the experimental estate of the Institute of Lowland Forestry and Environment.


2000 ◽  
Vol 14 (2) ◽  
pp. 382-385 ◽  
Author(s):  
L. M. Clayton ◽  
E. J. Milner‐Gulland ◽  
D. W. Sinaga ◽  
A. H. Mustari

2017 ◽  
Vol 24 (4) ◽  
pp. 209-216
Author(s):  
S. Salamma ◽  
A. Narayanaswamy ◽  
M. Naik ◽  
D. Veeranjaneyulu ◽  
M.V. Babu ◽  
...  

The population of Croton scabiosus, an endemic tree species of southern Eastern Ghats of Andhra Pradesh, India was assessed through random sampling in 15 localities spread over 8 locations. In sampled 37.5 h area, a total of 8737 mature individuals of Croton scabiosus was recorded. Of the 8 locations, Sanipaya sub population was found stable and considered elite owing to the maximum number of mature individuals, girth and height. In spite of good adult population, low number of seedlings and saplings of the species across the native terrain indicates its poor germination and recruitment warranting both in situ and ex situ conservation measures.


2019 ◽  
Vol 26 (3) ◽  
pp. 145-150
Author(s):  
Chitra Chandrasenan Rajeswary ◽  
◽  
S. Bindu ◽  
S.M. Shareef ◽  
V.S. Hima ◽  
...  

Seeds are the most effective and thriving propagation material of Salacia. As part of ex-situ conservation programme, four highly sought Salacia species, viz., Salacia brunoniana Wight & Arn., Salacia fruticosa Wall. Salacia malabarica Gamble and Salacia oblonga Wall. through fruit, seed and seedling characterization was carried out. For this, phenology and morphology of fruits and seeds with reference to polymorphism were documented. Effect of fresh and desiccated moisture content especially that of critical moisture were tested to understand the extent of viability of seeds. Since, these species became threatened in their natural habitat; attempts were made to standardize their seed germination characters and seedlings were raised. Seedling characters along with seedling vigour were documented up to 6 leaf stage and also an identification key was made based on their seedling characters which would aid in the demarcation of the species at their juvenile stage.


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