Effect of Seed Priming with Micro-Nutrients, Botanicals on Seedling Characteristics of Sorghum (Sorghum bicolor L. Moench) Var. Paiyur 2

The experiment was conducted in Seed Testing Laboratory, Department of Genetics and Plant Breeding, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj (U.P.) during kharif season 2019-2021, so as to standardize the acceptable seed treatments for sorghum (Paiyur-2).Different seed priming treatments were taken into the study viz., were evaluated T1 – Zinc Sulphate @ 0.5% up to 3hours, T2 – Zinc Sulfate@1% at 4 hours, T3 – Zinc [email protected]% at 6 hours, T4 – Thiourea @ 0.5% at 3 hours, T5 – Thiourea @ 1% at 4 hours, T6 – Thiourea @ 1.5% at 6 hours, T7 – KNO3@ 0.5% at 3 hours, T8 – KNO3 @ 1% at 4 hours, T9 – KNO3 @ 1.5% at 6 hours, T10 – Moringa Leaf extract @ 3% at 8 hours, T11 – Neem leaf extract @ 3% at 8 hours, T12 – Thiourea @0.5% with Zinc Sulphate(ZnSo4) @ 0.5% at 6 hours. During this study it had been found that maximum germination percent, seedling length and vigour attributes was observed when seed treated with T10- Moringa leaf extract @ 3% followed by T9 – KNO3 @ 1.5% and minimum observed in Control (Untreated seed). Seed treatment with Moringa leaf extract @ 3% enhances germination on seedling characters and it is a simple method and no requirement for expensive equipment and chemical might be used for overcoming challenges associated with a poor germination and seedling establishment.

The Effect of Hormone Treatments on Germination and Seedling Characters of Sage (Salvia officinalis L.) Seeds

Medicinal Sage is consumed as tea in sore throat and kidney diseases caused by cold and flu. It also has sedative, diuretic, antiperspirant and disinfectant effects. Thujone, which is found in the essential oil of Salvia officinalis species, is an essential oil component with very strong antiseptic and antibiotic effects. Sage (Salvia officinalis), which is a medicinal and an aromatic plant and has a wide area of usage, is cultivated due to these properties. However, the most critical cost item in the production of sage is the weeding done in the first years. The understory weeding done without using herbicides continues until the sage seedlings shield the soil and prevent the development of other herbs. The aim of this research was to determine the effects of hormone treatment on germination success and seedling morphological characters in sage seeds. Within the scope of this research, sage seeds were planted by being exposed to IAA, IBA, GA3 and NAA hormones at 1000, 2500 and 5000 ppm concentrations for 3 to 5 seconds and at 50, 100 and 200 ppm doses for 24 hours, and thus 26 applications were performed together with the control groups. The seeds were planted in sterile peat medium after the hormone treatments, and the effect of hormone treatments on the germination percentage and some seedling characters was tried to be found after 30 days of germination. As a result of the research, it was found that the hormone treatments positively affected most of seedling characters.

Seed priming for alleviation of heavy metal toxicity in plants: An overview

Heavy metal (HM) toxicity is vital environmental constraint that limits crop productivity worldwide. Several physiological processes necessary for plant survival have been found to be affected by HM toxicity. In recent farming, advanced mechanisms are being developed to overcome from the stresses to enhance the yield. The seed priming is an affordable method for plants to survive under abiotic and biotic stresses. Priming is useful for commercial seed lots by seed technologists to increase the vigor of the seeds in terms of germination potential and enhance the tolerance against various stresses. It also removes the pollution threats by minimizing the uses of chemical fertilizers. The seeds having deprived of quality in terms of seed germination and seedling characters ultimately affect the growth, photosynthetic performance and yield of the plants under HM stress. On the other hand seed primed with various seed priming methods such as hydropriming, hormonal priming, chemical priming, biopriming, magnetopriming and nanopriming perform well under HM toxicity. Seed priming methods have been considered as a unique approach to get rid of HM stress by enhancing the seed germination, seedling vigor, rate of photosynthesis, biomass accumulation and thus increase the crop productivity. The present review provides an overview of different seed-priming methods and their role in alleviation of adverse effects of HM stress in plants.

POLYPLOIDY INDUCED BY COLCHICINE IN Robinia pseudoacacia L. AND IT'S EFFECTS ON MORPHOLOGICAL, PHYSIOLOGICAL AND ANATOMICAL SEEDLING TRAITS

This study was aimed to induce and produce a polyploidy Robinia pseudoacacia seedling by different concentrations of colchicine (0.0%, 0.5%, 1.0%, 1.5%, and 2.0%) in various exposure times (12, 24 and 48 h), using in two experiments. In the first, were applied to the seeds by immersing method. In the second, were applied to shoot apical meristems by dropping method. The results showed significant effects of different interactions between colchicine concentration and exposure period on the seed germination, seedling survival rate and seedling characters. By increasing of the colchicine concentration the seed germination and seedling survival rate decreased significantly in colchicine concentrations (0.15 and 0.2%). Frequency of stomata decreased and on the opposite the stomata length and width increased with increases the colchicine concentrations. Polyploidy seedlings show vegetative growth superiority yield compared to diploids seedlings, leaf area in treated plants was larger and with deep green pigmentation as compared to the control seedlings. Seed treatment method was found to be more efficient than shoot apical meristems treatment method in producing tetraploid and mixoploid plants, colchicine solutions at 0.1% and 1.5% in 24 h. and 48 h. was found most effectively produced polyploidy and inducing variation in both investigations.