Effects of Inorganic Nitrogen Sources and Physical Factors on the Formation of Ubiquinone by Tobacco Plant Cells in Suspension Culture

Physiological studies pertaining to the effect of physical factors, carbon and nitrogen requirement of Phlebiopsis gigantea were conducted to know its behaviour in vitro. These studies have revealed interesting results regarding its growth and reproduction behaviour. The best medium for the optimum growth is Glucose-peptone medium. The optimum temperature (28°C) and pH (5.0) is required for the optimum mycelial production of Phlebiopsis gigantea after 12 days of incubation. The best carbon source for the growth of the fungus is D(+)Glucose least seen in Sucrose medium. The best inorganic nitrogen sources for the growth of the fungus is Sodium nitrate. The least mycelial growth of fungus is observed in ammonium acetate and ammonium nitrate. The best organic nitrogen compound for the mycelial growth of fungus is DL-threonine.

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Factors influencing cucumber (Cucumis sativus L.) somatic embryogenesis. I. The crucial role of pH and nitrogen in suspension culture

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.1995.029 ◽

2014 ◽

Vol 64 (3) ◽

pp. 223-231 ◽

Cited By ~ 11

Author(s):

Tadeusz Wróblewski ◽

Marcin K. Filipecki ◽

Stefan Malepszy

Keyword(s):

Suspension Culture ◽

Cucumis Sativus ◽

Ph Value ◽

Inorganic Nitrogen ◽

Nitrogen Sources ◽

Medium Composition ◽

High Yield ◽

Type I ◽

Cucumis Sativus L ◽

Stable Suspension

A method of obtaining and the characteristics of an embryogenic stabilised cucumber (Cucumis sativus L.) suspension culture which has many similarities to the carrot model are presented. The Specific Type I cells and proembryogenic mass were present in such a suspension. The maintenance of the proembryogenic stage took place in medium containing 2,4-D as the sole growth regulator, subsequent stages of embryogenesis occurred in hormone-free medium. Embryonic structures were also observed in medium with auxin in the late stages of growth, probably due to the depletion of 2,4-D in the medium during subculture. The choice of the proper inorganic nitrogen sources and the maintenance of correct proportions between them had a significant effect on the formation of these structures. We have shown that the pH of the medium with an embryogenic culture became stabilized regardless of the initial pH value and depended on the medium composition. The inoculum used for the initiation of subsequent subcultures of the stable suspension culture was 1 part tissue to 300 parts medium and was small in comparison to the systems described for the cucumber so far. From 1 ml of basic suspension 7 embryos were obtained on medium without growth regulators 10 days after inoculation, and this amount increased to 21 after 3 weeks. From 3.2% of the somatic embryos it was posible to regenerate plants. The high yield and synchronisation of the process and the development of embryos without passing through callus tissue create the possibility of using this system for molecular investigations and in the technology of somatic seed production.

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