scholarly journals Nutritional characteristics and physiological studies on the growth and development of Phlebiopsis gigantea

2017 ◽  
Vol 8 ◽  
Author(s):  
Sushma Negi ◽  
Indu Bhushan Prasher ◽  
Rajeev Kumar
Keyword(s):  
Mycelial Growth ◽  
Inorganic Nitrogen ◽  
Nitrogen Compound ◽  
Nitrogen Sources ◽  
Physical Factors ◽  
Phlebiopsis Gigantea ◽  
Nutritional Characteristics ◽  
Physiological Studies ◽  
Peptone Medium

<p>Physiological studies pertaining to the effect of physical factors, carbon and nitrogen requirement of <em>Phlebiopsis gigantea</em> were conducted to know its behaviour <em>in vitro</em>. These studies have revealed interesting results regarding its growth and reproduction behaviour. The best medium for the optimum growth is Glucose-peptone medium. The optimum temperature (28°C) and pH (5.0) is required for the optimum mycelial production of <em>Phlebiopsis gigantea</em> after 12 days of incubation. The best carbon source for the growth of the fungus is D(+)Glucose least seen in Sucrose medium. The best inorganic nitrogen sources for the growth of the fungus is Sodium nitrate. The least mycelial growth of fungus is observed in ammonium acetate and ammonium nitrate. The best organic nitrogen compound for the mycelial growth of fungus is DL-threonine.</p>

scholarly journals Effect of Nitrogen on in Vitro Propagation of Endangered Medicinal Plant: Swertia Chirayita Roxb. Ex Flaming

2021 ◽  
Vol 11 (2) ◽  
pp. 3513-3522
Keyword(s):  
Average Length ◽  
Inorganic Nitrogen ◽  
Nitrogen Sources ◽  
Multiple Shoots ◽  
Garden Soil ◽  
Equal Proportion ◽  
Ms Medium ◽  
Root Explants ◽  
Swertia Chirayita

The effect of nitrogen was investigated on the organogenesis of Swertia chirayita (Gentianaceae) to overcome the challenges related to its cultivation. The best callogenic response was observed on root explants inoculated onto MS medium supplemented with BAP (2.0 mg/l) along with 2,4-D (0.5 mg/l) after 35 days of culture. Subsequent transfer of callus for multiplication on the same media composition under complete darkness presents the best results in terms of callus multiplication. Callogenic cultures were subculture onto modified MS medium supplemented with inorganic nitrogen sources, i.e., NH4NO3 (14-56N/l), KNO3 (100-400N/l) with BAP (3.0 mg/l) were observed. Organogenic response (52%) was observed after 8-12 weeks of culturing. The maximum number of the shoot was recorded on MS medium with NH4NO3 (28 N/l), KNO3 (300N/l) with BAP (3.0 mg/l). Moreover, 90% of them were able to regrow when sub-cultured on the same media. Sixteen weeks old multiple shoots were subcultured on MS medium supplemented with different auxins. IAA was proved to be the best hormone rooting purpose. However, the best rooting response regarding the number of roots and an average length of roots was obtained at IAA (1.0 mg/l). Survival of 90% was achieved when rooted plantlets were successfully established in substrate containing sand, vermicompost, and garden soil in equal proportion for hardening and acclimatized.

scholarly journals Factors influencing mycelial growth of Sclerotium rolfsii

2013 ◽  
Vol 1 ◽  
pp. 26-31 ◽  
Author(s):  
Imdramani Bhagat
Keyword(s):  
Mycelial Growth ◽  
Organic Nitrogen ◽  
Inorganic Nitrogen ◽  
Sclerotium Rolfsii ◽  
Nitrogen Sources ◽  
Maximum Growth ◽  
Optimum Conditions ◽  
Factors Influencing ◽  
The World ◽  
Better Than

Sclerotial blight of tea (Camellia sinensis L.) caused by Sclerotium rolfsii Sacc. is one of the destructive diseases in tea growing areas of the world. In the present investigation, an attempt was made to know the optimum conditions for the mycelial growth of S. rolfsii. Factors influencing mycelial growth of S. rolfsii were studied with special reference to their growth in different media, variable pH and variable sources of carbon (viz., 6 types) as well as organic (viz., 4 types) and inorganic (viz., 4 types) nitrogen sources. Maximum growth of pathogen occurred after 8 days of inoculation at pH 6. Dextrose was the most effective carbon source and yeast extract (organic source) was found most optimum for growth of S. rolfsii. Organic nitrogen sources were found to be better than inorganic nitrogen sources. DOI: http://dx.doi.org/10.3126/njbs.v1i0.7466 Nepalese Journal of Biosciences 1: 26-31 (2011)

scholarly journals Nitrate Promotes Capsaicin Accumulation inCapsicum chinenseImmobilized Placentas

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Jeanny G. Aldana-Iuit ◽  
Enrique Sauri-Duch ◽  
María de Lourdes Miranda-Ham ◽  
Lizbeth A. Castro-Concha ◽  
Luis F. Cuevas-Glory ◽  
...  
Keyword(s):  
Amino Acids ◽  
Structural Damage ◽  
Inorganic Nitrogen ◽  
Placental Tissue ◽  
Nitrogen Sources ◽  
Nitrate Content ◽  
Tissue Sections ◽  
Scanning Electron Microcopy ◽  
Branched Amino Acids

In chili pepper’s pods, placental tissue is responsible for the synthesis of capsaicinoids (CAPs), the compounds behind their typical hot flavor or pungency, which are synthesized from phenylalanine and branched amino acids. Placental tissue sections from Habanero peppers (Capsicum chinenseJacq.) were immobilized in a calcium alginate matrix and culturedin vitro, either continuously for 28 days or during two 14-day subculture periods. Immobilized placental tissue remained viable and metabolically active for up to 21 days, indicating its ability to interact with media components. CAPs contents abruptly decreased during the first 7 days in culture, probably due to structural damage to the placenta as revealed by scanning electron microcopy. CAPs levels remained low throughout the entire culture period, even though a slight recovery was noted in subcultured placentas. However, doubling the medium’s nitrate content (from 40 to 80 mM) resulted in an important increment, reaching values similar to those of intact pod’s placentas. These data suggest that isolated pepper placentas culturedin vitroremain metabolically active and are capable of metabolizing inorganic nitrogen sources, first into amino acids and, then, channeling them to CAP synthesis.

scholarly journals INFLUENCE OF VARIOUS MEDIA AND NUTRIENT SOURCES ON ALTERNARIA SOLANI CAUSE EARLY BLIGHT DISEASE IN TOMATO

2021 ◽  
Vol 21 (no 1) ◽  
Author(s):  
B. Rex ◽  
G. Rajasekar
Keyword(s):  
Mycelial Growth ◽  
Leaf Extract ◽  
Early Blight ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Alternaria Solani ◽  
Dry Weight ◽  
Potato Dextrose Broth ◽  
Nutrient Sources

Early blight of tomato (Solanum lycopersicum L.) incited by Alternaria solani is highly destructive causing yield loss up to 78 per cent. The fungus was tested with different media along with the host extract and different nutrient sources for their growth and development in in vitro. Twelve different media were tested on the growth of A. solani, among them potato dextrose agar + host leaf extract recorded maximum radial mycelial growth of A. solani (89.57mm) and potato dextrose broth + leaf extract has maximum mycelial dry weight (613mg). Six carbon and nitrogen sources amended media were tested. Among carbon sources, glucose recorded maximum radial mycelial growth (74.65mm) and mycelia dry weight (709.17mg). Among the nitrogen sources, ammonium nitrate has the enhanced the radial mycelail growth (84.56 mm) and high mycelial dry weigh (654.27mg). This study will be helpful for further investigations on the physiology of the fungus and management of the disease.

Organic and inorganic nitrogen effects on spore production and mycelial growth of Neonectria ditissima in vitro

2018 ◽  
Vol 71 ◽  
pp. 354
Author(s):  
Rebecca E. Campbell ◽  
Amandine Touron ◽  
Monika Walter
Keyword(s):  
Mycelial Growth ◽  
Morphological Changes ◽  
Inorganic Nitrogen ◽  
Spore Production ◽  
In Planta ◽  
Inorganic N ◽  
Causal Organism ◽  
N Sources ◽  
Plant Pathogen Interaction

Nitrogen (N) is known to influence the growth of Neonectria ditissima (N.d.), the causal organism of European canker. In vitro, inorganic N inhibits conidia germination at N concentrations above 0.2 mol/L while, in planta, foliar urea application increased disease expression of leaf scar wounds up to nine-fold. The influence of organic and inorganic N sources on mycelial growth and spore production of N.d. in vitro was investigated. Four organic and six inorganic N sources were tested on agar at concentrations of N between 0 and 0.2 mol/L, with 3 different N.d. isolates. Spore production was generally increased by the addition of low concentrations of N, with varying results at higher concentrations dependent on the N source; however, this also differed among N.d. isolates. Spore production was generally incompletely inhibited at the higher N concentrations tested. However, germination from the resulting conidia decreased, possibly due to morphological changes to the spores. Mycelial growth generally decreased with the addition of N. Understanding the N effect in planta will be further complicated by the physiological plant-N and plant-pathogen interaction processes.

Effect of the addition of nitrogen sources to cassava fiber and carbon-to-nitrogen ratios on Agaricus brasiliensis growth

2007 ◽  
Vol 53 (1) ◽  
pp. 139-143 ◽  
Author(s):  
T R.D Mantovani ◽  
G A Linde ◽  
N B Colauto
Keyword(s):  
Ammonium Sulfate ◽  
Agaricus Bisporus ◽  
Mycelial Growth ◽  
C:N Ratio ◽  
Fungal Growth ◽  
Nitrogen Sources ◽  
Agaricus Brasiliensis ◽  
Axenic Cultivation ◽  
Better Than

The same substratum formulation to grow Agaricus bisporus has been used to grow Agaricus brasiliensis since its culture started in Brazil. Despite being different species, many of the same rules have been used for composting or axenic cultivation when it comes to nitrogen content and source in the substrate. The aim of this study was to verify the mycelial growth of A. brasiliensis in different ammonium sulfate and (or) urea concentrations added to cassava fiber and different carbon-to-nitrogen (C:N) ratios to increase the efficiency of axenic cultivation. Two nitrogen sources (urea and (or) ammonium sulfate) added to cassava fiber were tested for the in vitro mycelial growth in different C:N ratios (ranging from 2.5:l to 50:l) in the dark at 28 °C. The radial mycelial growth was measured after 8 days of growth and recorded photographically at the end of the experiment. Nitrogen from urea enhanced fungal growth better than ammonium sulfate or any mixture of nitrogen. The best C:N ratios for fungal growth were from 10:l to 50:l; C:N ratios below 10:l inhibited fungal growth.Key words: Agaricus brasiliensis, Agaricus blazei, Agaricus subrufescens, nitrogen, C:N ratio.

scholarly journals Optimum vegetative growth conditions and genetic diversity in different strains of Pleurotus salmoneastramineus L.J.N. Vassilzeva

2020 ◽  
Vol 49 (1) ◽  
pp. 125-134
Author(s):  
Nuhu Alam ◽  
Farhana Rahman
Keyword(s):  
Genetic Diversity ◽  
Mycelial Growth ◽  
Culture Media ◽  
Inorganic Nitrogen ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Its Region ◽  
Growth Conditions ◽  
Malt Extract ◽  
Arbitrary Primers

This experiment was undertaken to depict the favourable condition for mycelial growth, molecular identification and phylogenetic relationship of the selected strains of Pleurotus salmoneostramineus. Suitable temperature and pH were obtained at 25ºC and 6, respectively. Mushroom complete, glucose peptone and yeast malt extract culture media were favorable, while Hennerberg and Hoppkins were unfavorable. Dextrin was the best and xylose was the less effective carbon sources. Inorganic nitrogen sources were less effective for the mycelial growth of P. salmoneostramineus. The sequences of internal transcribed spacer (ITS) region of selected strains revealed that the total length ranged from 614 to 663 bp. The size of the ITS1 and ITS2 regions varied among the strains. Sequence analysis showed that 5.8S of rDNA sequences were identical. Phylogenetic tree of the ITS region sequences indicated that strains of P. salmoneostramineus belong to same cluster. The reciprocal homologies of the ITS region sequences ranged from 98 to 100%. The strains of P. salmoneostramineus were also analyzed by random amplification of polymorphic DNA (RAPD) with 20 arbitrary primers. RAPD results suggested that tested strains of P. salmoneostramineus were genetically similar with some variations, thus it could be concluded that RAPD and ITS techniques were well competent for detecting the genetic diversity of all tested strains of P. salmoneostramineus.

scholarly journals Influence of Carbon - Nitrogen Supplements and pH on Growth of Sugarcane Stem rot Pathogen Fusarium Solani NVS671

2020 ◽  
Vol 1 (1) ◽  
pp. 27-32
Author(s):  
Prittesh Patel
Keyword(s):  
Fusarium Solani ◽  
Mycelial Growth ◽  
Growth Promotion ◽  
Nitrogen Sources ◽  
Cultural Analysis ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
N Sources ◽  
Sugarcane Stem

Fusarium solani NVS671 identified from infected sugarcane stem of Co 671 as a new pathogen was subjected to various cultural conditions to understand its physiological profile. In continuation with our previous work, cultural analysis was carried out under in vitro condition by supplementing various carbon and nitrogen sources in Czapek-Dox agar (CDA). Under different hydrogen ion concentrations, it was found that the growth of Fusarium solaniNVS671 was less at pH 4 and pH 10 and could reach up to 5 cm after 7 days of incubation on Potato Dextrose Agar (PDA). It is observed that the pH around 7 to 8 was optimum for the growth of F. solani. Ten different nitrogenous (N) sources and nine different carbon sources were tested on CDA medium to know their effect on the mycelial growth rate and characteristics of the fungus. Among the N sources evaluated, ammonium chloride (7.96±0.11 cm) was found to be most efficient for mycelial growth promotion followed by ammonium nitrate (7.7±0.1 cm) and ammonium sulphate (7.3±0.1 cm). The most preferred carbon source recorded to promote best radial mycelial growth was starch (7.96±0.05 cm) and sucrose (7.93±0.05 cm). Capabilities of using different carbon and nitrogen sources and ability to grow at different pH levels may allow species to adapt to specific soil conditions.This study is important to understand the physiology and metabolite preference of F. solani.

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