Monoclonal Antibody-Based Fluorescence Polarization Immunoassay for Furazolidone in Feed

2010 ◽  
Vol 43 (17) ◽  
pp. 2716-2729 ◽  
Author(s):  
Shiwei Zhang ◽  
Yudong Shen ◽  
Yuanming Sun
1992 ◽  
Vol 38 (1) ◽  
pp. 123-126 ◽  
Author(s):  
M Winkler ◽  
G Schumann ◽  
D Petersen ◽  
M Oellerich ◽  
K Wonigeit

Abstract In a prospective study we evaluated a novel fluorescence polarization immunoassay (FPIA) for determining cyclosporine (CsA) in whole blood. FPIA uses a monoclonal antibody and is performed on the TDx (Abbott). The within-series (CV less than 2%) and between-days (CV less than 3.3%) precision of the assay was excellent. The results obtained by the monoclonal FPIA in samples from transplant patients (n = 100) averaged 31.9% and 20.2% higher than those by HPLC and a specific radioimmunoassay (INCStar), respectively. Results by all three methods correlated well. Follow-up studies during the early course after liver transplantation, however, suggested that high metabolite concentrations affect FPIA results. This is explained by previously described cross-reactions of the monoclonal antibody with some CsA metabolites. The FPIA results in samples of such patients should be interpreted cautiously.


Food Control ◽  
2019 ◽  
Vol 105 ◽  
pp. 38-44 ◽  
Author(s):  
Yingshan Chen ◽  
Qiyi He ◽  
Ding Shen ◽  
Zhengyun Jiang ◽  
Sergei A. Eremin ◽  
...  

2007 ◽  
Vol 55 (17) ◽  
pp. 6871-6878 ◽  
Author(s):  
Zhanhui Wang ◽  
Suxia Zhang ◽  
Irina S. Nesterenko ◽  
Sergei A. Eremin ◽  
Jianzhong Shen

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