Serum protein fractions in acute falciparum malaria obtained by polyacrylamide gel electrophoresis

1978 ◽  
Vol 72 (1) ◽  
pp. 3-12 ◽  
Author(s):  
P. Migasena ◽  
F.P. Schelp ◽  
N. Vudhivai ◽  
P. Boonperm ◽  
V. Supawan ◽  
...  
Author(s):  
Suprava Roy ◽  
Y. Vasudeva Rao

Background: In the present study attempts have been made to study the blood cells and serum proteins comparatively among three different varieties of fowls, namely Turkey, RIR and Kadaknath. Total RBC and WBC count and serum protein analysis were performed. Methods: The total RBC and WBC were counted with the help of Neubauer’s Haemocytometer. The serum proteins were separated and analysed by native polyacrylamide gel electrophoresis (PAGE). Result: The results showed that, among all the fowl varieties tested, the RBC counts were ranged from 3.01 to 4.46 millions/mm3 and the WBC counts were ranged from 18.26 to 21.78 thousands/mm3. The serum protein analysis revealed that some proteins are present in specific species and specific sex. Hence, these proteins may be counted as markers to determine the species and sex of the species.


2011 ◽  
Vol 47 (1) ◽  
pp. 119-123
Author(s):  
Cristiane Maria Colli ◽  
Guita Rubinsky Elefant ◽  
Marcia Liz Paludo ◽  
Ana Lúcia Falavigna Guilherme

The aim of the present study was to test the effectiveness of a sausage-casing membrane for dialysis of Toxocara excretory-secretory antigens (TES). The protein concentrated by the tested membrane was compared with that obtained using a Sigma commercial membrane, as were the protein fractions found by polyacrylamide gel electrophoresis. Standard positive and negative serum samples were evaluated in an ELISA immunoassay, and equivalent data were obtained in all steps, indicating that the sausage-casing membrane is efficient, besides being less expensive to process.


1987 ◽  
Vol 1987 (15) ◽  
pp. 22-27
Author(s):  
Michinari Yokohama ◽  
Yasuko Watanabe ◽  
Hitoshi Gawahara ◽  
Etsuko Kobayashi

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