Changes of Western honey bee Apis mellifera ligustica (Spinola, 1806) ventriculus microbial profile related to their in-hive tasks

2020 ◽  
Vol 60 (1) ◽  
pp. 198-202
Author(s):  
Giovanni Cilia ◽  
Filippo Fratini ◽  
Elena Tafi ◽  
Simone Mancini ◽  
Barbara Turchi ◽  
...  
2020 ◽  
Vol 7 (2) ◽  
pp. 76 ◽  
Author(s):  
Giovanni Cilia ◽  
Filippo Fratini ◽  
Elena Tafi ◽  
Barbara Turchi ◽  
Simone Mancini ◽  
...  

The effects of veterinary drugs, dietary supplements and non-protein amino acids on the European honey bee (Apis mellifera ligustica Spinola, 1806) ventriculum microbial profile were investigated. Total viable aerobic bacteria, Enterobacteriaceae, staphylococci, Escherichia coli, lactic acid bacteria, Pseudomonas spp., aerobic bacterial endospores and Enterococcus spp. were determined using a culture-based method. Two veterinary drugs (Varromed® and Api-Bioxal®), two commercial dietary supplements (ApiHerb® and ApiGo®) and two non-protein amino acids (GABA and beta-alanine) were administered for one week to honey bee foragers reared in laboratory cages. After one week, E. coli and Staphylococcus spp. were significantly affected by the veterinary drugs (p < 0.001). Furthermore, dietary supplements and non-protein amino acids induced significant changes in Staphylococcus spp., E. coli and Pseudomonas spp. (p < 0.001). In conclusion, the results of this investigation showed that the administration of the veterinary drugs, dietary supplements and non-protein amino acids tested, affected the ventriculum microbiological profile of Apis mellifera ligustica.


2015 ◽  
Vol 59 (2) ◽  
pp. 27-36 ◽  
Author(s):  
Marco Porporato ◽  
Giacomo Grillone ◽  
Augusto Patetta ◽  
Aulo Manino ◽  
Daniela Laurino

AbstractWhile observing: non-acceptance, frequent replacements, and reduced performance in honey bee (Apis mellifera ligustica) queens, we were induced to analyse a certain number of queens to detect the causes. For this purpose, 99 newly mated queens were bought from 20 Italian queen breeders. In addition, 109 older or at-the-end-of-their-career queens, that showed poor productivity, were collected from honey production hives throughout Italy. All the queens were dissected to check the status of their reproductive system and/or the presence of various anomalies and diseases.Anatomical and functional anomalies, pertaining to ovaries and spermatheca, also in association with tissue alterations and microorganisms like Nosema spp. and protozoa, were common in both newly mated and older queens, but they were more prevalent in the latter. Observed differences permitted us to highlight the causes of the reduced performance of the queens and of the colonies they originated from. The obtained information could prove useful when it comes to improving queen breeding techniques.


2014 ◽  
Vol 13 (9) ◽  
pp. 2306-2320 ◽  
Author(s):  
Yu Fang ◽  
Mao Feng ◽  
Bin Han ◽  
Xiaoshan Lu ◽  
Haitham Ramadan ◽  
...  

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