staphylococcus spp
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2023 ◽  
Vol 83 ◽  
M. Mushtaq ◽  
S. M. Bukhari ◽  
S. Ahmad ◽  
A. Khattak ◽  
M. B. Chattha ◽  

Abstract There is a paucity of research conducted on microbial prevalence in pheasants. The microbiota of captive birds has zoonotic significance and must be characterize. Present study is therefore planned to assess the microbiota from oral, fecal and gut content of captive avian species. It will be helpful in characterization of harmful microbes. Different samples taken from oral, gut and feces of ring-necked pheasants (Phasianus colchicus), green pheasants (Phasianus versicolor), golden pheasant (Chrysolophus pictus) and silver pheasant (Lophura nycthemera). Samples were collected, diluted, and inoculated onto different agar plates (MacConkey, SS agar, MSA and nutrient agar) for cultivation of bacterial species. Colonies of E.coli, Staphylococcus spp. Brachyspira spp. and Campylobacter spp were observed based on colony morphology. Colony forming unit showed E. coli as frequently found bacteria in fecal, oral and gut contents of all the above pheasants. The overall significance difference was found among bacterial species of golden pheasants, green pheasant, ring-necked pheasant, and silver pheasants. It was concluded that E.coli is predominant isolated from heathy pheasants followed by Campylobacter, Staphylococcus and Brachyspira.

2022 ◽  
Vol 43 (2) ◽  
pp. 901-910
Lilian Bernardina Ferreira ◽  
Larissa de Freitas Santiago Israel ◽  
Renata Fernandes Rabello ◽  
Guilherme Nunes de Souza ◽  

Staphylococcus bacteria are often associated with subclinical bovine mastitis. This study aimed to identify multiresistant Staphylococcus spp. associated with subclinical mastitis and the associated risk factors. Twenty-three dairy farms with a history of decrease in milk production, located in the lower Acre region, Brazil, were selected. An epidemiological questionnaire was provided in all farms. All animals were examined using the California Mastitis Test (CMT) and their milk samples were collected for bacterial culture. After isolation and identification, the disk diffusion antimicrobial susceptibility test was performed against nine classes of antimicrobials. Of the 339 cows examined using the CMT, 108 had mastitis. A total of 229 milk samples were collected from individual teats. MALDI-TOF MS found isolates belonging to eight species of Staphylococcus, in 101 of these samples. S. chromogenes (58.4%) demonstrated strongest resistance to the nine classes of antimicrobial active principles. Nineteen isolates with multidrug resistance phenotypic profile were identified. This phenotypic expression indicates wide circulation of resistant genes in this species. The presence of multidrug resistance in Staphylococcus spp. in this study was correlated with lack of water for cleaning the corral, which is a preventive factor, minimizing the transmission and persistence of pathogens in the farms.

Megha Pande ◽  
Suresh Kumar ◽  
Y.K. Soni ◽  
N. Prasad ◽  
N. Chand ◽  

Background: Repeat breeding syndrome (RBS) associated with sub-clinical uterine infection (UI) remains to be a major reproductive problem faced by Indian farmers. Present study documents its diagnosis, prevalent etiological agents, antibiogram pattern and efficacy of the treatment at field level. Methods: Seventy-eight RBS affected bovines were selected. The cervical mucous (CM) was collected for study of its characteristics, white-side test, endometrial cytology, microbial examination and antibiogram. The treatment protocol was developed and the animals’ response to the treatment was assessed. Result: The overall incidence of RBS was found to be 12.9% and the cases associated with uterine infection (RBS/UI+ve) and without uterine infection (RBS/UI-ve) were 44.87% and 55.13%, respectively. The mean scores of CM character, odour, pH and number of polymorphonuclear cells in RBS/UI +ve were 2.09±1.39, 1.14±0.12, 8.49±0.08 and 12.46±0.96, respectively, and differed significantly (P less than 0.05) from RBS/UI-ve cases. The microbial examination revealed the presence of gram negative bacilli, Trueperella spp., Pseudomonas spp., Staphylococcus spp., Escherichia coli and yeast infection. Antibiogram studies recorded the response of Tetracyclin as best (48.57%) followed by Cephalexin (22.86%), Chloramphenicol (20.0%) and Streptomycin (8.57%). The RBS/UI+ve cases were treated individually, on one-to-one basis, obeying antibiogram. The infection appeared to be considerably controlled and overall success rate was observed in the form of confirmed pregnancy in 71.43% cases. Thus, it was concluded that prompt diagnosis using endometrial cytology and antibiogram guided therapeutic approach may aid for effective management of RBS/UI +ve cases, under field conditions.

2022 ◽  
Vol 17 (4) ◽  
pp. 28-34
D. M. Sharifullina ◽  
O. K. Pozdeev ◽  
R. M. Vasileva ◽  
R. N. Khayrullin

Aim of the study was to assess the frequency of microflora detection in the blood of patients with atherosclerotic vascular lesions and middle-aged patients without clinical symptoms of atherosclerosis. Compare the nature of microflora isolated from blood and the range of microorganisms isolated from atherosclerotic plaques (AP) of patients with carotid arteries atherosclerosis. Material and methods. The hemocultures of 118 men and 33 women with atherosclerosis (mean age 55.6 years) as well as 10 blood samples of 3 men and 7 women formed into a control group (mean age 37 years) were examined. Test samples were cultivated for 6 months. Tissue platings of carotid arteries AP in 11 women and 24 men of the main group (mean age 58.0) were incubated for 2 months. Methods based on Student’s t-test and Mann-Whitney U-test were used for statistical analysis of the obtained results. Results. Propionibacterium acnes hemocultures were detected in 9.9 % of patients from the main and control groups. In blood Staphylococcus epidermidis and Stenotrophomonas maltophylia were detected in 0.7 % of samples. P. acnes and Staphylococcus spp. cultures were obtained from AP in 34.3 and 45.7 %, including both microorganisms in 8.6 % of samples. Conclusions. P. acnes cultures are found equally frequently in the blood of atherosclerotic patients and patients not yet diagnosed with atherosclerosis. This study confirmed the presence of the same-name microorganisms in blood and atherosclerotic plaques. In 5.7 % (2 of 35) it was possible to extract simultaneously a P. acnes culture from two loci (atherosclerotic tissue and blood) in specific individuals. Further detailed research is required to study the etiological significance of the microbial factor in the atherosclerotic plaque formation.

2022 ◽  
Vol 21 (1) ◽  
pp. 14-27
M. L. SAWA ◽  

It emerges from bacteriological study that it is possible to isolate many bacterial types and yeasts from the lesions of footrot infection in sheep. Anaerobic cultivation came out with the isolation of Spherophorus spp. (24%), Bacteroides spp. (60%), Corynebacterium spp. (64%), Enterobacteracae (76%), Streptococcus spp. (76%), Staphylococcus spp. (36%), Clostridum sordellii (46%) and Irichosporon cutaneum (4%).  On the other hand aerobic cultivation rendered the isolation of Corynebacterium spp. (100%), Entrobacteracae (100%), Staphylococcus spp. (15%), Penicillium spp., Aspergillus fumigatus and Trichosporon cutaneum.  Five different drug combinations were studied for their efficacy in the treatment of ovine footrot. Their healing rates were as follows : oxytetracycline (59%), oxytetracyclin with formaline (70.9%), Procaine penicillin and streptomycin (72.5%), Procaine penicillin and streptomycin with formaline dipping (80.76%), Formaline alone (63.8%). All kinds of treatment indicated statistically significant differences to exit between the treated (experimental) and untreated (control) groups

2022 ◽  
Vol 43 (1) ◽  
pp. 141-158
Mauricio Fanin ◽  
Isabela Carvalho dos Santos ◽  
Geysiane Moreira Gerotti ◽  
Camila de Cuffa Matusaiki ◽  

Milk and its derivatives are highly consumed foods worldwide, with recognized nutritional importance. The search for the production of products with superior quality is constant. For the present work, 26 milk-producing properties were selected, with a total of 506 milk samples collected during the period from October 2019 to May 2020 being evaluated. The objective of this study was to evaluate the quality of milk produced in dairy properties in the region west Paraná, classified as good or bad based on the results of the Somatic Cell Count (SCC) and through sampling (n = 10) to evaluate the resistance profile of enterobacteria and Staphylococcus spp. isolated from milk samples, in addition to the presence of the mecA gene in strains of Staphylococcus spp. resistant to oxacillin. There were significant differences between the good and bad properties for the levels of lactose, SCC (cell/mL), and Standard Plate Count (SPC) (CFU/mL). The strains of Staphylococcus spp. showed differences in the percentage of resistance in relation to the good and bad properties for antibiotics: tetracycline, ciprofloxacin, oxacillin, amikacin, clindamycin, gentamycin, and erythromycin. The mecA gene was not detected in any of the coagulase-negative Staphylococcus isolates that showed resistance to oxacillin. For enterobacteria, the isolated species differed in relation to the classification of properties, with predominance for Escherichia coli (40%) for properties classified as bad and Hafnia alvei (40%) for those classified as good. The percentage of antibiotic resistance compared to enterobacteria isolates was higher in properties classified as good. Monitoring through microbial culture and antibiogram is extremely important, favoring the correct choice for the treatment of animals with a reduced selection of resistant strains.

2022 ◽  
Vol 508 (2) ◽  
Nguyễn Thị Nhung ◽  
Lưu Thị Bình

Mục tiêu: Mô tả đặc điểm lâm sàng, cận lâm sàng và căn nguyên vi khuẩn học của bệnh nhân nhiễm khuẩn tiết niệu (NKTN) phức tạp. Đối tượng và phương pháp nghiên cứu: Mô tả cắt ngang 100 bệnh nhân được chẩn đoán NKTN phức tạp tại Bệnh viện Trung ương Thái Nguyên (BVTWTN) từ tháng 02/2020 đến tháng 8/2021. Kết quả: Tỷ lệ nam/nữ = 0,79. Tuổi trung bình của các bệnh nhân là 61,64±17,15 với 60% bệnh nhân ≥60 tuổi. Thời gian nằm viện trung bình của các bệnh nhân là 14,22±7,49 ngày với 87% bệnh nhân nằm viện >7 ngày. 41% bệnh nhân có biểu hiện sốt, 33% với tiểu buốt và 49% với đau hông lưng. 41% bệnh nhân có suy thận, 40% với sỏi thận và 32% với bệnh lý suy giảm miễn dịch. 100% bệnh nhân có bạch cầu trong nước tiểu, 44% có hồng cầu niệu và 45% có nitrit niệu dương tính. Cấy nước tiểu: 91% dương tính với vi khuẩn Gram âm, trong đó vi khuẩn E.coli chiếm 61%, P.aeruginosa là 8%; E.coli nhạy cảm cao với fosfomycin (93,8%), meropenem (93,2%), piperacillin + tazobactam (84,9%) và amikacin (71,2%); kháng >50% các kháng sinh nhóm fluoroquinolone và các thế hệ của cephalosporin, kháng >80% nhóm betalactam và ức chế acid folic. Vi khuẩn Gram dương phân lập được hai chủng là Staphylococcus spp (4%) và Enterococcus spp (5%). Trong đó, Staphylococcus spp đã kháng với hầu hết các nhóm kháng sinh đang được sử dụng tại bệnh viện. Kết luận: NKTN phức tạp thường gặp ở những bệnh nhân cao tuổi, có bệnh lý thận mạn tính, sỏi tiết niệu hoặc tình trạng suy giảm miễn dịch. Triệu chứng lâm sàng thường gặp là sốt, rối loạn đi tiểu. Căn nguyên vi khuẩn gây NKTN phức tạp thường gặp là E.coli. Nhiều vi khuẩn có tỷ lệ đề kháng cao với các kháng sinh đang được sử dụng để điều trị NKTN tại bệnh viện.

2022 ◽  
Vol 11 (1) ◽  
pp. e32811124855
Rubens Barbosa Rezende ◽  
Isadora Moreira Costa do Nascimento Nogueira

Objetivou-se avaliar a prevalência e o perfil de sensibilidade aos antimicrobianos dos gêneros Klebsiella spp. e Staphylococcus spp. isolados de amostras de urinas de mulheres provenientes de um Laboratório privado de Análises Clínicas no município de Conselheiro Lafaiete-MG, bem como a estação de maior prevalência. Foi realizado um estudo observacional transversal, do tipo quantitativo, visando à coleta de dados secundários sobre a prevalência e o perfil de sensibilidade dos gêneros Klebsiella spp. e Staphylococcus spp. isolados das uroculturas positivas em pacientes do gênero feminino com faixa etária entre 18 a 60 anos, no período de janeiro a dezembro de 2019. No período do estudo foram identificadas neste laboratório 41 uroculturas positivas provenientes de mulheres dentro da faixa etária estipulada, sendo o gênero Klebsiella spp. o mais prevalente 13/41 (31,71%), seguido do Staphylococcus spp. 11/41 (26,83%). A partir dos resultados obtidos neste estudo, foi possível identificar que todos os objetivos foram alcançados, uma vez que se obteve o número de casos de infecções em pacientes do gênero feminino entre 18 a 60 anos, foi avaliado a estação com maior prevalência de infecção do trato urinário, apresentou-se em tabelas os patógenos de interesse, e descrito o perfil de sensibilidade das bactérias aos principais antimicrobianos de relevância clínica.

2022 ◽  
Vol 11 (1) ◽  
pp. e26811124826
Vinícius Moura Andrade ◽  
Ana Maria de Resende Machado ◽  
Fátima de Cássia Oliveira Gomes

O queijo parmesão é consumido pela população na forma ralada, o que tem proporcionado uma maior opção de marcas do produto no mercado. No entanto, o queijo é um produto susceptível a contaminações, oferecendo riscos à Saúde Pública. Poucos estudos sobre esse assunto têm sido publicados na literatura, tornando-se importante averiguar a qualidade dos produtos disponíveis no mercado. Assim, o trabalho teve por objetivo avaliar a qualidade microbiológica, determinar o teor de umidade e o perfil de compostos voláteis de diferentes amostras embaladas e a granel de queijo parmesão ralado, disponíveis no comércio, verificando se os produtos atendem aos padrões estabelecidos pela legislação. Em relação a umidade, observou-se que 50% das amostras apresentaram valores acima do permitido (20 g/100 g). Nas análises microbiológicas verificou-se que 70% das amostras estavam impróprias para o consumo com contagens acima do permitido pela legislação para bolores e leveduras, Staphylococcus spp. e coliformes totais. Em algumas amostras foram identificadas a presença de coliformes termotolerantes e Escherichia coli, mas não ultrapassaram o padrão estabelecido pela legislação. A análise dos compostos voláteis permitiu a identificação de 31 compostos de diferentes classes como cetonas (7), ésteres (7), ácidos carboxílicos (6), aldeídos (4), álcoois (2), lactonas (2), hidrocarboneto aromático (1), terpeno (1) e triacetina (1).  Observou-se variação na composição química entre os queijos embalados e a granel. Essas variações podem ser observadas na falta de uniformidade das amostras o que requer a necessidade de uma fiscalização mais eficiente pelos órgãos oficiais e melhorias nas condições higiênicas durante a fabricação.

Animals ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 117
Cecilia Kellerman ◽  
Pongpreecha Malaluang ◽  
Ingrid Hansson ◽  
Lena Eliasson Selling ◽  
Jane M. Morrell

Extenders for boar semen contain antibiotics, which may induce antimicrobial resistance (AMR) in inseminated females. The objective was to investigate AMR of bacteria isolated from the cervix of sows and gilts in standing heat, representing females previously exposed to antibiotics in the semen extender and non-exposed females, respectively. Cervical swabs were taken from 30 multiparous sows and 30 gilts prior to their first insemination. After culturing on agar plates, bacterial isolates were identified by Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry and antimicrobial minimum inhibitory concentrations (MIC) were determined. Differences in antibiotic resistance between sows and gilts were analyzed by Chi-squared or Fisher’s exact test. Bacteria isolated were mostly Staphylococcus spp., Streptococcus spp. and Corynebacterium spp. Higher MICs were observed for isolates from sows than from gilts. Most (>80%) Corynebacterium spp. were resistant to clindamycin; small numbers (<20%) were resistant to gentamicin, penicillin, vancomycin, ciprofloxacin and rifampicin, with no differences between gilts and sows. Corynebacterium from gilts were more often resistant to tetracycline than those from sows (25% vs. 4.17%; p = 0.04). In conclusion, bacteria from the porcine cervix showed low resistance to most antibiotics except for clindamycin, but antibacterial resistance may increase with increasing parity.

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