Structure and functional analyses of bacterial communities changes in microbial mats following petroleum exposure

Summary The bacterial communities in the wound tissues of Pinus massoniana were analysed by 16S rDNA amplicon sequencing. The results showed that the bacterial richness and diversity changed remarkably whether the wound was inoculated with pine wood nematode (PWN; Bursaphelenchus xylophilus) or not after 12 h. However, the predominant bacteria Stenotrophomonas, Burkholderiaceae, Pseudomonas, Serratia and Delftia, introduced by PWN in the wound tissues, changed within 6 h. After 6 h of PWN inoculation, the most abundant genus associating with PWN, Stenotrophomonas, failed to colonise the wound tissues, and the abundance of Delftia decreased, while the other representative bacteria, Burkholderiaceae, Pseudomonas and Serratia, from the PWN were markedly enriched. In addition, our study is the first to report the association of Serratia liquefaciens with PWN. Predicted functional analyses using the Tax4Fun tool showed that the alterations in bacterial composition also led to shifts in their functional pathways, especially after 12 h of PWN inoculation. These findings clarified that the bacteria carried by PWN were responsible for the alterations in bacterial communities in the wound tissues and will shed light on the invasion mechanism of PWN.

Download Full-text

Thermophilic bacterial communities inhabiting the microbial mats of “indifferent” and chalybeate (iron-rich) thermal springs: Diversity and biotechnological analysis

MicrobiologyOpen ◽

10.1002/mbo3.560 ◽

2017 ◽

Vol 7 (2) ◽

pp. e00560 ◽

Cited By ~ 6

Author(s):

Ramganesh Selvarajan ◽

Timothy Sibanda ◽

Memory Tekere

Keyword(s):

Bacterial Communities ◽

Microbial Mats ◽

Thermal Springs

Download Full-text

Niche differentiation of bacterial communities at a millimeter scale in Shark Bay microbial mats

Scientific Reports ◽

10.1038/srep15607 ◽

2015 ◽

Vol 5 (1) ◽

Cited By ~ 59

Author(s):

Hon Lun Wong ◽

Daniela-Lee Smith ◽

Pieter T. Visscher ◽

Brendan P. Burns

Keyword(s):

Bacterial Communities ◽

Microbial Mats ◽

Niche Differentiation ◽

Shark Bay

Download Full-text

Structure, dynamics and predicted functional ecology of the gut microbiota of the blue (Haliotis fulgens) and yellow (H. corrugata) abalone from Baja California Sur, Mexico

10.7287/peerj.preprints.3233v1 ◽

2017 ◽

Cited By ~ 1

Author(s):

Francesco Cicala ◽

José Alejandro Cisterna-Céliz ◽

James Douglas Moore ◽

Axayácatl Rocha-Olivares

Keyword(s):

Gut Microbiota ◽

Bacterial Communities ◽

Metabolic Pathways ◽

Structural Changes ◽

Functional Ecology ◽

Bacterial Assemblage ◽

Metabolic Functions ◽

Specific Variation ◽

Haliotis Corrugata ◽

Functional Analyses

The gastro-intestinal (GI) microbiota of abalone contains a highly complex bacterial assemblage playing an essential role in the overall health of these gastropods. The gut bacterial communities characterized so far reveal considerable interspecific variability, likely resulting from bacterial interactions and constrained by the ecology of their host species; however, they remain poorly investigated. Additionally, the extent to which structural changes in the microbiota entail functional shifts in metabolic pathways of bacterial communities remains unexplored. In order to address these questions, we characterized the gut microbiota of the northeast Pacific blue (Haliotis fulgens or HF) and yellow (Haliotis corrugata or HC) abalone by 16S rRNA 454 pyrosequencing to shed light on: (i) their gut microbiota structure; (ii) how bacteria may interact among them; and (iii) predicted shifts in bacterial metabolic functions associated with the observed structural changes. Our findings revealed that Mycoplasma dominated the GI microbiome in both species. However, the structure of the bacterial communities differed significantly in spite of considerable intra-specific variation. This resulted from differences of the species with most reads in each GI metagenome, suggesting host-specific adaptation of bacterial lineages to these sympatric abalone. We hypothesize that the presence of exclusive OTUs in each microbiota may relate to host-specific differences in competitive pressure. Significant differences in bacterial diversity were found for the explored metabolic pathways between species despite their functional overlap. A more diverse array of bacteria contributed to each function in HC, whereas a single or much fewer OTUs were generally observed in HF. The structural and functional analyses allowed us to describe a deep taxonomic and functional split between the microbiota of HF and HC abalone.

Download Full-text

Effect of seasonality and physicochemical parameters on bacterial communities in two hot spring microbial mats from Araró, Mexico

Revista Mexicana de Biodiversidad ◽

10.1016/j.rmb.2017.07.010 ◽

2017 ◽

Vol 88 (3) ◽

pp. 616-624 ◽

Cited By ~ 12

Author(s):

Cristina M. Prieto-Barajas ◽

Ruth Alfaro-Cuevas ◽

Eduardo Valencia-Cantero ◽

Gustavo Santoyo

Keyword(s):

Bacterial Communities ◽

Physicochemical Parameters ◽

Microbial Mats ◽

Hot Spring

Download Full-text

Microbial Diversity of a Heavily Polluted Microbial Mat and Its Community Changes following Degradation of Petroleum Compounds

Applied and Environmental Microbiology ◽

10.1128/aem.68.4.1674-1683.2002 ◽

2002 ◽

Vol 68 (4) ◽

pp. 1674-1683 ◽

Cited By ~ 150

Author(s):

Raeid M. M. Abed ◽

Nimer M. D. Safi ◽

Jürgen Köster ◽

Dirk de Beer ◽

Yasser El-Nahhal ◽

...

Keyword(s):

Microbial Community ◽

Microbial Diversity ◽

Significant Role ◽

Bacterial Communities ◽

Bacterial Population ◽

Microbial Mats ◽

Community Response ◽

Microbial Mat ◽

Cyanobacterial Mats ◽

Community Changes

ABSTRACT We studied the microbial diversity of benthic cyanobacterial mats inhabiting a heavily polluted site in a coastal stream (Wadi Gaza) and monitored the microbial community response induced by exposure to and degradation of four model petroleum compounds in the laboratory. Phormidium- and Oscillatoria-like cyanobacterial morphotypes were dominant in the field. Bacteria belonging to different groups, mainly the Cytophaga-Flavobacterium-Bacteriodes group, the γ and β subclasses of the class Proteobacteria, and the green nonsulfur bacteria, were also detected. In slurry experiments, these communities efficiently degraded phenanthrene and dibenzothiophene completely in 7 days both in the light and in the dark. n-Octadecane and pristane were degraded to 25 and 34% of their original levels, respectively, within 7 days, but there was no further degradation until 40 days. Both cyanobacterial and bacterial communities exhibited noticeable changes concomitant with degradation of the compounds. The populations enriched by exposure to petroleum compounds included a cyanobacterium affiliated phylogenetically with Halomicronema. Bacteria enriched both in the light and in the dark, but not bacteria enriched in any of the controls, belonged to the newly described Holophaga-Geothrix-Acidobacterium phylum. In addition, another bacterial population, found to be a member of green nonsulfur bacteria, was detected only in the bacteria treated in the light. All or some of the populations may play a significant role in metabolizing the petroleum compounds. We concluded that the microbial mats from Wadi Gaza are rich in microorganisms with high biodegradative potential.

Download Full-text

Molecular diversity studies of bacterial communities of oil polluted microbial mats from the Etang de Berre (France)

FEMS Microbiology Ecology ◽

10.1111/j.1574-6941.2006.00187.x ◽

2006 ◽

Vol 58 (3) ◽

pp. 550-562 ◽

Cited By ~ 65

Author(s):

Guillermina Hernandez-Raquet ◽

Pierre Caumette ◽

Patrick Dabert ◽

...

Keyword(s):

Bacterial Communities ◽

Molecular Diversity ◽

Microbial Mats ◽

Diversity Studies

Download Full-text

Structure, dynamics and predicted functional role of the gut microbiota of the blue (Haliotis fulgens) and yellow (H. corrugata) abalone from Baja California Sur, Mexico

PeerJ ◽

10.7717/peerj.5830 ◽

2018 ◽

Vol 6 ◽

pp. e5830 ◽

Cited By ~ 5

Author(s):

Francesco Cicala ◽

José Alejandro Cisterna-Céliz ◽

James D. Moore ◽

Axayácatl Rocha-Olivares

Keyword(s):

Gut Microbiota ◽

Bacterial Communities ◽

Metabolic Pathways ◽

Structural Changes ◽

Bacterial Assemblage ◽

Metabolic Functions ◽

Abalone Species ◽

Haliotis Corrugata ◽

Functional Analyses

The GI microbiota of abalone contains a highly complex bacterial assemblage playing an essential role in the overall health of these gastropods. The gut bacterial communities of abalone species characterized so far reveal considerable interspecific variability, likely resulting from bacterial interactions and constrained by the ecology of their abalone host species; however, they remain poorly investigated. Additionally, the extent to which structural changes in the microbiota entail functional shifts in metabolic pathways of bacterial communities remains unexplored. In order to address these questions, we characterized the gut microbiota of the northeast Pacific blue (Haliotis fulgensor HF) and yellow (Haliotis corrugataor HC) abalone by16S rRNAgene pyrosequencing to shed light on: (i) their gut microbiota structure; (ii) how bacteria may interact among them; and (iii) predicted shifts in bacterial metabolic functions associated with the observed structural changes. Our findings revealed thatMycoplasmadominated the GI microbiome in both species. However, the structure of the bacterial communities differed significantly in spite of considerable intraspecific variation. This resulted from changes in predominant species composition in each GI microbiota, suggesting host-specific adaptation of bacterial lineages to these sympatric abalone. We hypothesize that the presence of exclusive OTUs in each microbiota may relate to host-specific differences in competitive pressure. Significant differences in bacterial diversity were found between species for the explored metabolic pathways despite their functional overlap. A more diverse array of bacteria contributed to each function in HC, whereas a single or much fewer OTUs were generally observed in HF. The structural and functional analyses allowed us to describe a significant taxonomic split and functional overlap between the microbiota of HF and HC abalone.

Download Full-text