Liquid Chromatographic Analysis for Flecainide with Use of a Microbore Column and Ultraviolet Detection

1988 ◽  
Vol 11 (4) ◽  
pp. 891-899 ◽  
Author(s):  
Thomas Annesley ◽  
Keith Matz
Keyword(s):  
Chromatographic Analysis ◽  
Ultraviolet Detection ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic ◽  
Microbore Column

Liquid-chromatographic analysis for cyclosporine with use of a microbore column and small sample volume.

1986 ◽  
Vol 32 (7) ◽  
pp. 1407-1409 ◽  
Author(s):  
T Annesley ◽  
K Matz ◽  
L Balogh ◽  
L Clayton ◽  
D Giacherio
Keyword(s):  
Chromatographic Analysis ◽  
Glass Tube ◽  
Sample Volume ◽  
Small Sample ◽  
Butyl Ether ◽  
Microbore Columns ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic ◽  
Microbore Column ◽  
Small Sample Volume

Abstract This liquid-chromatographic assay requires 0.2 to 0.5 mL of whole blood, avoids the use of diethyl ether, and consumes only 10 to 20% of the solvents used in prior methods. Sample preparation involves an acidic extraction with methyl-t-butyl ether, performed in a 13 X 100 mm disposable glass tube, then a short second extraction of the organic phase with sodium hydroxide. After evaporation of the methyl-t-butyl ether, chromatography is performed on an "Astec" 2.0-mm (i.d.) octyl column. We compared results by this procedure with those by use of earlier larger-scale extractions and their respective 4.6-mm (i.d.) columns; analytical recoveries of cyclosporins A and D were comparable with previous findings and results for patients' specimens were equivalent, but the microbore columns provided greatly increased resolution and sensitivity.

Determination of Ivermectin in Medicated Swine Feeds at the 2 ppm Concentration Level

1990 ◽  
Vol 73 (6) ◽  
pp. 931-934
Author(s):  
Stephen J Doherty ◽  
Allen Fox ◽  
David W Fink
Keyword(s):  
Chromatographic Analysis ◽  
Concentration Level ◽  
Reverse Phase ◽  
Ultraviolet Detection ◽  
Mean Relative Error ◽  
Relative Standard ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic ◽  
Medicated Feeds

Abstract An analytical method has been developed that Is applicable to the determination of Ivermectin in medicated feeds at the 2 ppm concentration level. It Is based upon liquid chromatographic analysis with a reverse-phase column and ultraviolet detection. After the drug Is extracted from the feed Into methanol, an analytical sample Is prepared by the consecutive use of column chromatography on alumina and solidphase extraction on Sep-Pak C18 and silica cartridges. This procedure has been applied to the concentration range 0.50- 3.0 ppm of Ivermectin In feed with an accuracy of ±2% mean relative error and a precision of ± 2% relative standard deviation at the 2 ppm concentration level.

Sign in / Sign up

Export Citation Format

Share Document