Gradient Elution Centrifugal Partition Chromatography: Comparison with HPLC Gradients and use of Ternary Diagrams to Build Gradients

1990 ◽  
Vol 13 (18) ◽  
pp. 3583-3602 ◽  
Author(s):  
Alain Foucault ◽  
Koji Nakanishi
Keyword(s):  
Gradient Elution ◽  
Partition Chromatography ◽  
Centrifugal Partition Chromatography ◽  
Ternary Diagrams

scholarly journals Bioassay-Guided Separation of Centipeda minima Using Comprehensive Linear Gradient Centrifugal Partition Chromatography

Molecules ◽  
2020 ◽  
Vol 25 (13) ◽  
pp. 3077
Author(s):  
Ji Hoon Kim ◽  
Eun Ju Jung ◽  
Yun Jung Lee ◽  
En Mei Gao ◽  
Ahmed Shah Syed ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Gradient Elution ◽  
Solvent System ◽  
Luciferase Assay ◽  
Partition Chromatography ◽  
Centrifugal Partition Chromatography ◽  
Lower Phase ◽  
Linear Gradient ◽  
Centipeda Minima ◽  
Water Saturated

A comprehensive linear gradient solvent system for centrifugal partition chromatography (CPC) was developed for the bioassay-guided isolation of natural compounds. The gradient solvent system consisted of three different ternary biphasic solvents types: n-hexane–acetonitrile–water (10:2:8, v/v), ethyl acetate–acetonitrile–water (10:2:8, v/v), and water-saturated n-butanol–acetonitrile–water (10:2:8, v/v). The lower phase of the n-hexane–acetonitrile–water (10:2:8, v/v) was used as the stationary phase, while its upper phase, as well as ethyl acetate–acetonitrile–water (10:2:8), and water-saturated n-butanol–acetonitrile–water (10:2:8, v/v) were pumped to generate a linear gradient elution, increasing the mobile phase polarity. We used the gradient CPC to identify antioxidant response elements (AREs), inducing compounds from Centipeda minima, using an ARE-luciferase assay in HepG2 cells, which led to the purification of the active molecules 3-methoxyquercetin and brevilin A. The developed CPC solvent systems allow the separation and isolation of compounds with a wide polarity range, allowing active molecule identification in the complex crude extract of natural products.

Modelling gradient elution in centrifugal partition chromatography

1997 ◽  
Vol 773 (1-2) ◽  
pp. 13-22 ◽  
Author(s):  
Michel J van Buel ◽  
Luuk A.M van der Wielen ◽  
Karel Ch.A.M Luyben
Keyword(s):  
Gradient Elution ◽  
Partition Chromatography ◽  
Centrifugal Partition Chromatography

Preparative separation of anthocyanins by gradient elution centrifugal partition chromatography

1997 ◽  
Vol 763 (1-2) ◽  
pp. 345-352 ◽  
Author(s):  
Jean-Hugues Renault ◽  
Philippe Thépenier ◽  
Monique Zéches-Hanrot ◽  
Louisette Le Men-Olivier ◽  
André Durand ◽  
...  
Keyword(s):  
Gradient Elution ◽  
Partition Chromatography ◽  
Centrifugal Partition Chromatography ◽  
Preparative Separation

Preparative separation of quinolines by centrifugal partition chromatography with gradient elution

2003 ◽  
Vol 1011 (1-2) ◽  
pp. 55-65 ◽  
Author(s):  
Philippe Duret ◽  
Mohamed Akram Fakhfakh ◽  
Christine Herrenknecht ◽  
Alain Fournet ◽  
Xavier Franck ◽  
...  
Keyword(s):  
Gradient Elution ◽  
Partition Chromatography ◽  
Centrifugal Partition Chromatography ◽  
Preparative Separation

Characterization of Proanthocyanidin-Rich Dentin Biomodifiers from Plants by Centrifugal Partition Chromatography

Planta Medica ◽  
2013 ◽  
Vol 79 (10) ◽  
Author(s):  
JW Nam ◽  
RS Phansalkar ◽  
JG Napolitano ◽  
SN Chen ◽  
JB McAlpine ◽  
...  
Keyword(s):  
Partition Chromatography ◽  
Centrifugal Partition Chromatography

One-step preparative isolation of aristolochic acids by strong ion-exchange centrifugal partition chromatography

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
AA Abdelgadir ◽  
L Boudesocque-Delaye ◽  
I Thery-Koné ◽  
A Gueiffier ◽  
EM Ahmed ◽  
...  
Keyword(s):  
Ion Exchange ◽  
Partition Chromatography ◽  
Centrifugal Partition Chromatography ◽  
Preparative Isolation ◽  
Aristolochic Acids ◽  
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