Production of cupcake-like dessert containing microbial biosurfactant as an emulsifier

This work describes the application of the biosurfactant from Candida bombicola URM 3718 as a meal additive like cupcake. The biosurfactant was produced in a culture medium containing 5% sugar cane molasses, 5% residual soybean oil and 3% corn steep liquor. The surface and interfacial tension of the biosurfactant were 30.790 ± 0.04 mN/m and 0.730 ± 0.05 mN/m, respectively. The yield in isolated biosurfactant was 25 ± 1.02 g/L and the CMC was 0.5 g/L. The emulsions of the isolated biosurfactant with vegetable oils showed satisfactory results. The microphotographs of the emulsions showed that increasing the concentration of biosurfactant decreased the oil droplets, increasing the stability of the emulsions. The biosurfactant was incorporated into the cupcake dessert formulation, replacing 50%, 75% and 100% of the vegetable fat in the standard formulation. Thermal analysis showed that the biosurfactant is stable for cooking cupcakes (180 °C). The biosurfactant proved to be promising for application in foods low in antioxidants and did not show cytotoxic potential in the tested cell lines. Cupcakes with biosurfactant incorporated in their dough did not show significant differences in physical and physical–chemical properties after baking when compared to the standard formulation. In this way, the biosurfactant has potential for application in the food industry as an emulsifier for flour dessert.

Remediation of Aviation Kerosene-Contaminated Soil by Sophorolipids from Candida bombicola CB 2107

Yeast-derived biosurfactants may substitute or complement chemical surfactants as green reagents to extract petroleum hydrocarbons from contaminated soil. The effectiveness of contaminant clean-up by sophorolipids was tested on kerosene-contaminated soil with reference to traditional synthetic surfactants. The sophorolipids produced by the yeast Candida bombicola CB 2107, cultivated with the carbon sources 10 g/L glucose and 10 g/L rapeseed oil, were most effective in contaminant removal. This biosurfactant revealed a critical micelle concentration of 108 mg/L which was close to that of Triton X-100 (103 mg/L), the synthetic surfactant considered as reference. It outperformed Triton X-100 in reducing kerosene concentrations (C10–C40) in contaminated soils. In a soil initially containing 1080 mg/kg of C10–C40, the concentration was reduced to 350 mg/kg using the biosurfactant, and to 670 mg/kg using Triton-X. In the soil with initial concentration of 472 mg/kg, concentrations were reduced to 285 and 300 mg/kg for biosurfactant and Triton X-100, respectively. Sophorolipids have the potential to replace synthetic surfactants. Properties and performance of the biosurfactants, however, strongly differ depending on the yeast and the growing conditions during production.

Production and characterization of sophorolipids by Candida bombicola using catfish fat

Catfish fat is a high nutrition by-product of seafood processing industry. In this work, catfish fat has been used as a sustainable and economical raw material for sophorolipids production by Candida bombicola. Sophorolipids yield was maximum as 21.8g/L after 7 days of fermentation at 25oC, pH 6, 180 rpm. The obtained sophorolipids was to contained the main component as lactonic sophorolipids, which has been confirmed by Thin layer chromatography (TLC). Sophorolipids also exhibited the ability to resistant Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa and showed the ability of free radical scavenging the dose dependent manner with IC50 was 4.45 mg/ml. These results suggested that sophorolipids could be used in health care products and cosmetic. Catfish fat could be used as the low cost hydrophobic carbon source to replace fatty acid for sophorolipids production.