Optimizing and scale-up strategy of molecular distillation for the purification of lactic acid from fermentation broth

Polylactide (PLA), which is one of the most important biocompatible polyesters that are derived from annually renewable biomass such as corn and sugar beets, has attracted much attention for automotive parts application. The manufacturing method of PLA is the ring-opening polymerization of the dimeric cyclic ester of lactic acid, lactide. For the stereocomplex PLA, we developed the four unit processes, fermentation, separation, lactide conversion, and polymerization. Fermentation of sugars to D-lactic acid is little studied, and its microbial productivity is not well known. Therefore, we investigated D-lactic acid fermentation with a view to obtaining the strains capable of producing D-lactic acid, and we got a maximum lactic acid production 60 g/L. Lactide is prepared by a two-step process: first, the lactic acid is converted into oligo(lactic acid) by a polycondensation reaction; second, the oligo(lactic acid) is thermally depolymerized to form the cyclic lactide via an unzipping mechanism. Through catalyst screening test for polycondensation and depolymerization reactions, we got a new method which shortens the whole reaction time 50% the level of the conventional method. Poly(L-lactide) was obtained from the ring-opening polymerization of L-lactide. We investigated various catalysts and polymerization conditions. Finally, we got the best catalyst system and the scale-up technology.

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A Process Study of Lactic Acid Production from Phragmites australis Straw by a Thermophilic Bacillus coagulans Strain under Non-Sterilized Conditions

Processes ◽

10.3390/pr6100175 ◽

2018 ◽

Vol 6 (10) ◽

pp. 175 ◽

Cited By ~ 3

Author(s):

Yuming Zhang ◽

Mengran Li ◽

Tian Nie ◽

Zhihua Ni

Keyword(s):

Lactic Acid ◽

Phragmites Australis ◽

Lactic Acid Production ◽

Scale Up ◽

Bacillus Coagulans ◽

Dilute Acid Pretreatment ◽

Integrated Process ◽

Dilute Acid ◽

Acid Pretreatment ◽

Process Study

Phragmites australis straw (PAS) is an abundant and renewable wetland lignocellulose. Bacillus coagulans IPE22 is a robust thermophilic strain with pentose-utilizing capability and excellent resistance to growth inhibitors. This work is focused on the process study of lactic acid (LA) production from P. australis lignocellulose which has not been attempted previously. By virtue of thermophilic feature of strain IPE22, two fermentation processes (i.e., separated process and integrated process), were developed and compared under non-sterilized conditions. The integrated process combined dilute-acid pretreatment, hemicellulosic hydrolysates fermentation, and cellulose utilization. Sugars derived from hemicellulosic hydrolysates and cellulose enzymatic hydrolysis were efficiently fermented to LA in a single vessel. Using the integrated process, 41.06 g LA was produced from 100 g dry PAS. The established integrated process results in great savings in terms of time and labor, and the fermentation process under non-sterilized conditions is easy to scale up for economical production of lactic acid from PAS.

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Lactic Acid Recovery From Cheese Whey Fermentation Broth Using Combined Ultrafiltration and Nanofiltration Membranes

Twenty-Seventh Symposium on Biotechnology for Fuels and Chemicals ◽

10.1007/978-1-59745-268-7_83 ◽

2007 ◽

pp. 985-996 ◽

Cited By ~ 1

Author(s):

Yebo Li ◽

Abolghasem Shahbazi

Keyword(s):

Lactic Acid ◽

Cheese Whey ◽

Fermentation Broth ◽

Nanofiltration Membranes ◽

Acid Recovery

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Extraction and separation of D/L-lactic acid in simulated fermentation broth

Korean Journal of Chemical Engineering ◽

10.1007/s11814-011-0010-z ◽

2011 ◽

Vol 28 (7) ◽

pp. 1608-1612 ◽

Cited By ~ 6

Author(s):

Jun Zhou ◽

Wentao Bi ◽

Kyung Ho Row

Keyword(s):

Lactic Acid ◽

Fermentation Broth ◽

Extraction And Separation

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Application of electrodialysis for lactic acid recovery

Czech Journal of Food Sciences ◽

10.17221/6579-cjfs ◽

2013 ◽

Vol 19 (No. 2) ◽

pp. 73-80 ◽

Cited By ~ 10

Author(s):

V. Hábová ◽

K. Melzoch ◽

M. Rychtera ◽

L. Přibyl ◽

V. Mejta

Keyword(s):

Lactic Acid ◽

Fermentation Broth ◽

Sodium Lactate ◽

Final Concentration ◽

Second Step ◽

Slow Flow ◽

Lactic Acid Concentration ◽

Initial Concentration ◽

Bipolar Membranes ◽

Acid Recovery

The paper deals with the possibility of using two-stage electrodialysis for recovery of lactic acid from model solutions and from fermentation broth. In the first step lactate was concentrated with desalting electrodialysis using ion exchange membranes Ralex (Mega,Czech Republic). The highest final concentration of 111 g/l was reached in the concentrate, it means an increase more than 2.5-times in comparison with the initial concentration. At the most 2 g of lactate per litre remained in the feed. The second step was the electroconversion of sodium lactate to lactic acid by water-splitting electrodialysis with the bipolar membranes Neosepta (Tokuyama Corp.,Japan). The final lactic acid concentration of 157 g/l was reached in the diluate. Total required energy in both electrodialysis processes consisting of the energy consumption for lactate transfer and for its electroconversion to lactic acid was 142 Wh/mol. The fermentation broth was decolourised before electrodialysis experiments. The best decolourisation capacity was shown by granulated active charcoal filled in the column operated by a slow flow of broth.

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Lactic acid production with the supplementation of spent cells and fish wastes for the purpose of reducing impurities in fermentation broth

Biochemical Engineering Journal ◽

10.1016/j.bej.2007.02.030 ◽

2007 ◽

Vol 36 (3) ◽

pp. 276-280 ◽

Cited By ~ 16

Author(s):

Min-Tian Gao ◽

Makoto Hirata ◽

Eiichi Toorisaka ◽

Tadashi Hano

Keyword(s):

Lactic Acid ◽

Acid Production ◽

Fermentation Broth ◽

Lactic Acid Production

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LACTIC ACID RECOVERY FROM CHEESE WHEY FERMENTATION BROTH USING NANOFILTRATION MEMBRANES

10.13031/2013.19661 ◽

2005 ◽

Author(s):

Yebo Li ◽

Abolghasem Shahbazi ◽

Seku Coulibaly ◽

Michele R. Mims

Keyword(s):

Lactic Acid ◽

Cheese Whey ◽

Fermentation Broth ◽

Nanofiltration Membranes ◽

Acid Recovery

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Reactive Extraction of Lactic Acid, Formic Acid and Acetic Acid from Aqueous Solutions with Tri-n-octylamine/1-Octanol/n-Undecane

ChemEngineering ◽

10.3390/chemengineering3020043 ◽

2019 ◽

Vol 3 (2) ◽

pp. 43 ◽

Cited By ~ 3

Author(s):

Nuttakul Mungma ◽

Marlene Kienberger ◽

Matthäus Siebenhofer

Keyword(s):

Mass Transfer ◽

Acetic Acid ◽

Lactic Acid ◽

Formic Acid ◽

Fermentation Broth ◽

Lactic Acid Production ◽

Mass Action ◽

Reactive Extraction ◽

Technical Application ◽

Pka Value

The present work develops the basics for the isolation of lactic acid, acetic acid and formic acid from a single as well as a mixed feed stream, as is present, for example, in fermentation broth for lactic acid production. Modelling of the phase equilibria data is performed using the law of mass action and shows that the acids are extracted according to their pka value, where formic acid is preferably extracted in comparison to lactic and acetic acid. Back-extraction was performed by 1 M NaHCO3 solution and shows the same tendency regarding the pka value. Based on lactic acid, the solvent phase composition, consisting of tri-n-octylamine/1-octanol/n-undecane, was optimized in terms of the distribution coefficient. The data clearly indicate that, compared to physical extraction, mass transfer can be massively enhanced by reactive extraction. With increasing tri-n-octylamine and 1-octanol concentration, the equilibrium constant increases. However, even when mass transfer increases, tri-n-octylamine concentrations above 40 wt%, lead to third phase formation, which needs to be prevented for technical application. The presented data are the basis for the transfer to liquid membrane permeation, which enables the handling of emulsion tending systems.

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