Electron Microscopic Characteristics of β2-Microglobulin Amyloid Deposits in Long-Term Haemodialysis

Since 1984 there have been reports of a destructive spondyloarthropathy occurring in patients on long-term hemodialysis. The primary abnormality appears to be an accumulation of β2-microglobulin, which is not adequately removed by dialysis, and forms amyloid deposits in articular and periarticular tissues. We report a case of this disease in a patient treated only by peritoneal dialysis. While this form of treatment may delay the development of arthropathy, as compared to hemodialysis, it does not prevent it. An increasing incidence of this disorder may be expected, since increasing numbers of patients have been on long-term peritoneal dialysis.

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Immunocytochemical Localization of Amyloid Protein AA in the “Dense Fibrillar Inclusions” of the Reticuloendothelical Cells

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100079632 ◽

1977 ◽

Vol 35 ◽

pp. 438-439

Author(s):

T. Shirahama ◽

M. Skinner ◽

A.S. Cohen

Keyword(s):

Amyloid Fibril ◽

Close Association ◽

Gel Filtration ◽

Fibril Formation ◽

Amyloid Protein ◽

Electron Microscopic ◽

Amyloid Deposits ◽

Amyloid Fibril Formation ◽

Electron Microscopic Technique ◽

Lysosomal System

A1thought the mechanisms of amyloidogenesis have not been entirely clarified, proteolysis of the parent proteins may be one of the important steps in the amyloid fibril formation. Recently, we reported that "dense fibrillar inclusions" (DFI), which had the characteristics of lysosomes and contained organized fibrillar profiles as well, were observed in the reticuloendothelial cells in close association with the foci of new amyloid deposits. We considered the findings as evidence for the involvement of lysosomal system in amyloid fibril formation (l). In the present study, we attempted to determine the identity of the contents of the DFI by the use of antisera against the amyloid protein (AA) and an immuno-electron microscopic technique.Amyloidosis was induced in CBA/J mice by daily injections of casein (l). AA was isolated from amyloid-laden spleens by gel filtration and antibody to it was produced in rabbits (2). For immunocytochemistry, the unlabeled antibody enzyme method (3) was employed.

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Amino acid sequence of a modified β2-microglobulin in renal failure patient urine and long-term dialysis patient blood

Clinica Chimica Acta ◽

10.1016/0009-8981(95)06039-g ◽

1995 ◽

Vol 236 (2) ◽

pp. 135-144 ◽

Cited By ~ 5

Author(s):

Tomoko Momoi ◽

Masami Suzuki ◽

Koiti Titani ◽

Shuichi Hisanaga ◽

Hiroshi Ogawa ◽

...

Keyword(s):

Renal Failure ◽

Amino Acid ◽

Amino Acid Sequence ◽

Dialysis Patient ◽

Renal Failure Patient ◽

Β2 Microglobulin

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Long-term Fate of Lung Autografts Charged with Providing Total Pulmonary Function I. Light and Electron Microscopic Studies

Annals of Surgery ◽

10.1097/00000658-197911000-00015 ◽

1979 ◽

Vol 190 (5) ◽

pp. 648-653 ◽

Cited By ~ 1

Author(s):

FRANK J. VEITH ◽

CHERYL M. MONTEFUSCO ◽

SIGURD BLUMCKE ◽

JACK W. C. HAGSTROM

Keyword(s):

Pulmonary Function ◽

Electron Microscopic ◽

Microscopic Studies

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Light and electron microscopic examination of endothelial cells from bovine retinal vessels in long-term cultures

Virchows Archiv ◽

10.1007/s004280050038 ◽

2000 ◽

Vol 436 (3) ◽

pp. 249-256 ◽

Cited By ~ 2

Author(s):

M. Sahm ◽

P. Seifert

Keyword(s):

Endothelial Cells ◽

Microscopic Examination ◽

Electron Microscopic Examination ◽

Retinal Vessels ◽

Electron Microscopic

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Long-term monitoring of microleakage of composites. Part II: Scanning electron microscopic examination of replica patterns of composite tags

Journal of Prosthetic Dentistry ◽

10.1016/0022-3913(88)90247-8 ◽

1988 ◽

Vol 60 (4) ◽

pp. 451-458 ◽

Cited By ~ 3

Author(s):

A.C. Shortall

Keyword(s):

Microscopic Examination ◽

Electron Microscopic Examination ◽

Electron Microscopic ◽

Scanning Electron Microscopic ◽

Long Term Monitoring ◽

Term Monitoring ◽

Scanning Electron

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MO921PROGNOSTIC OUTCOMES OF TRANSPLANTED KIDNEY USING SOLUBLE CD30 AND Β2- MICROGLOBULIN

Nephrology Dialysis Transplantation ◽

10.1093/ndt/gfab112.003 ◽

2021 ◽

Vol 36 (Supplement_1) ◽

Author(s):

Ahmed Abo omar ◽

Gamal Saadi

Keyword(s):

Acute Rejection ◽

Graft Survival ◽

Patient Group ◽

The Other ◽

Kidney Graft ◽

Post Transplantation ◽

Β2 Microglobulin ◽

Transplanted Kidney ◽

Successful Transplantation

Abstract Background and Aims Transplantation is the first successful modality of renal replacement therapy (RRT) for irreversible chronic kidney disease (CKD; stage 5). Identifying additional factors associated with poor long-term prognosis after transplantation may provide clues regarding the pathophysiological mechanisms involved in allograft failure and identify high-risk patients who may benefit from additional monitoring or interventions. Successful kidney transplantation results in a substantial decrease in β2M levels, but a delayed decrease or increasing levels after transplantation may serve as a marker of acute rejection or inflammation. Several reports show that elevated sCD30 levels, pre and post transplantation are associated with a poor prognosis for long term kidney graft survival. These studies found higher CD30 levels in allograft recipients and a good predictor of impending acute rejection. The aim of the work is to study the prognostic outcomes of transplanted kidney using CD30 and β2-Microglobulin Method prospective study was conducted in nephrology unit –internal medicine department at Tanta and Kasr El Ainy university ,over 1 year.20 patients subjected to primary Tx.participated in this study.Cd30 and β2M.at day -1,2weeks and 3 months,with clinical follow up after 1 year to detect graft survival Results At day -1,level of cd30 was higher in rejection group than the other patient group.2 weeks post transplantation ,level of cd30 was higher in rejection group than the other patient group and at 3 monthes post transplantation level of cd30 was higher in rejection group than the other patient group,and these differences are statistically highly significant.(p values :0.003 ,0.005 and 0.002 respectively) Successful transplantation leads to significant decrease in serum cd30 at 2 weeks post tx.(P1 <0.005) and at 3 monthes post tx. (P1<0.001) although in rejection group, significant decrease in cd30 was at 2 weeks post tx.only(P1<0.005) and at 3 monthes serum cd30 began to rise again with( P1 0.157). At day -1,level of β2microglobulin was higher in rejection group than the other patient groupwith statistically significant difference (p. 0.01).2 weeks post transplantation ,level of β2microglobulin was higher in rejection group than the other patient groupbut statistically not significant(p. 0.18 ) and at 3 monthes post transplantation level of β2microglobulin was higher in rejection group than the other patient group but statistically non significant(p. 0.18 ). Successful transplantation leads to significant decrease in serum β2microglobulin at 2 weeks post tx.(P1 <0.002) and at 3 monthes post tx. (P1<0.001) although in rejection group ,significant decrease in β2microglobulin was at 3 monthes post tx.only(P1<0.005) and at 2 weeks no significant decrease(p1 0.15) Conclusion pre transplantation high Cd30 and β2M is associated with poor outcome.failure of decrease of cd30 and β2M post Tx. also associated with poor outcome or infection. Successful transplantation leads to significant decrease in serum cd30 and β2M. which can be used as predictors of graft survival with better sensitivity and specificity than serum creatinin.

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Macroglobulinemia in a Child With Acute Leukemia

Blood ◽

10.1182/blood.v43.2.191.191 ◽

1974 ◽

Vol 43 (2) ◽

pp. 191-199 ◽

Cited By ~ 8

Author(s):

Jan Čejka ◽

Robert O. Bollinger ◽

Henrica R.E. Schuit ◽

Jeanne M. Lusher ◽

Chung-Ho Chang ◽

...

Keyword(s):

Acute Leukemia ◽

Immunoglobulin M ◽

Leukemia Cells ◽

Bence Jones Protein ◽

Electron Microscopic ◽

Lymphoid Leukemia ◽

Β2 Microglobulin ◽

Immunofluorescence Studies ◽

Bence Jones Proteinuria ◽

Serum And Urine

Abstract A 12-yr-old boy with acute leukemia was found to have paraproteinemia and Bence-Jones proteinuria. The paraprotein was characterized as immunoglobulin M, type κ and the Bence Jones protein as free κ-chains. Increased amounts of β2-microglobulin were found in the patient’s serum and urine. Electron microscopic and immunofluorescence studies provided evidence of the presence of lymphoid leukemia cells apparently responsible for the production of the paraprotein.

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Longitudinal Study of Proteins in Plasma and Dialysate during Continuous Ambulatory Peritoneal Dialysis (CAPD)

Peritoneal Dialysis International ◽

10.1177/089686089001000402 ◽

1990 ◽

Vol 10 (4) ◽

pp. 257-261 ◽

Cited By ~ 6

Author(s):

Gerald A. Young ◽

Albert Taylor ◽

Steven Kendall ◽

Aleck M. Brownjohn

Keyword(s):

Peritoneal Dialysis ◽

Longitudinal Study ◽

Membrane Permeability ◽

Continuous Ambulatory Peritoneal Dialysis ◽

Plasma Proteins ◽

Complement C3 ◽

Acid Glycoprotein ◽

Β2 Microglobulin ◽

Male Patients

The aim was to evaluate plasma proteins during continuous ambulatory peritoneal dialysis (CAPD) in relation to dialysis losses, membrane permeability, renal insufficiency, and time on CAPD. Ten male patients, established on CAPD for at least 14 months, were studied every 8 weeks for 56 weeks. Blood and dialysate from the morning exchange were analysed for urea, creatinine, and 7 proteins, and used to calculate dialysate to plasma concentration ratios (DIP). These ratios were not significantly changed suggesting that permeability remained constant. However, there was a trend for β2-microglobulin, creatinine, and urea to increase progressively. After 56 weeks, β2-microglobulin had increased from 27.9 to 31.3 mglL (p < 0.05) and creatinine 1006 to 1099 μmoLIL (p < 0.05) and both correlated with time on CAPD (p < 0.001). Plasma α1-acid glycoprotein, albumin, transferrin, IgG, IgA, and complement C3 were not significantly changed, although IgA and complement C3 were each negatively correlated with time on CAPD (r = −0.70 and −0.67, respectively), creatinine (r = 0.51 and −0.54), and urea (r = −0.61 and −0.61) (p < 0.001 for all). It is concluded that increases in β2-microglobulin, creatinine, and urea are not due to loss of membrane permeability but reflect a slight increase in uraemia. Long-term decreases in immunological proteins may be caused by uraemia or progressive depletion.

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