Newer Emerging Pathogens of Ocular Non-Sporulating Molds (NSM) Identified by Polymerase Chain Reaction (PCR)-Based DNA Sequencing Technique Targeting Internal Transcribed Spacer (ITS) Region

2008 ◽  
Vol 33 (2) ◽  
pp. 139-147 ◽  
Author(s):  
R. Bagyalakshmi ◽  
K. L. Therese ◽  
S. Prasanna ◽  
H. N. Madhavan
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Sequencing ◽  
Internal Transcribed Spacer ◽  
Its Region ◽  
Emerging Pathogens ◽  
Chain Reaction ◽  
Sequencing Technique ◽  
Polymerase Chain

scholarly journals Detection of Pythium ultimum Using Polymerase Chain Reaction with Species-Specific Primers

Plant Disease ◽  
1997 ◽  
Vol 81 (10) ◽  
pp. 1155-1160 ◽  
Author(s):  
K. Kageyama ◽  
A. Ohyama ◽  
M. Hyakumachi
Keyword(s):  
Polymerase Chain Reaction ◽  
Internal Transcribed Spacer ◽  
Pcr Amplification ◽  
Its Region ◽  
Pythium Ultimum ◽  
Pythium Species ◽  
Chain Reaction ◽  
Specific Primers ◽  
Polymerase Chain ◽  
Species Specific

This study was conducted to sequence the rDNA internal transcribed spacer (ITS) region of Pythium ultimum and Pythium group HS, design species-specific primers for polymerase chain reaction (PCR), and detect P. ultimum from diseased seedlings using PCR. The sequence of the ITS region of P. ultimum was identical with that of Pythium group HS. The results support the reports that the HS group is an asexual strain of P. ultimum. Using PCR, the primer pair K1+K3, designed on portions of the sequence of the ITS region, amplified isolates of P. ultimum and the HS group but not isolates of 20 other Pythium species. DNA extracts from damped-off seedlings were not amplified, but a 10-fold dilution of the extracts with Tris-EDTA (TE) buffer diluted the inhibitors and allowed PCR amplification. The primer pair used detected P. ultimum from a single diseased seedling.

scholarly journals Puces à ADN comme outil d'identification moléculaire pour Culicoides

2009 ◽  
Vol 62 (2-4) ◽  
pp. 144
Author(s):  
J.C. De Witte ◽  
I. Deblauwe ◽  
Gill De Deken ◽  
R. De Deken ◽  
M. Madder ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Internal Transcribed Spacer ◽  
Culicoides Species ◽  
Chain Reaction ◽  
Diagnostic Laboratory ◽  
Laboratory Equipment ◽  
Naked Eye ◽  
Culicoides Obsoletus ◽  
Polymerase Chain ◽  
Obsoletus Complex

A DNA microarray test based on internal transcribed spacer 1 (ITS1) genotype expression was developed to identify Culicoides species of Northwestern Europe belonging to the Culicoides Obsoletus complex. The assay was designed so as to allow interpretation by the naked eye. False positive and false nega­tive results were eliminated. The need for expensive laboratory equipment and reagents was kept as low as possible, making the technique affordable and feasible for any diagnostic laboratory with polymerase chain reaction (PCR) facilities. The microarray test could be validated through the three ringtests organised by Medreonet. Use of this microarray can improve monitoring adult and immature Culicoides species.

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