A simple spectrophotometric method to determine phytic acid in poultry wastewater without acid digestion

Abstract We compared a spectrophotometric kit method (Glycospec) for determination of glycosylated hemoglobin (HbA1) with a microcolumn kit method (Bio-Rad). The Glycospec method is based on the change in absorbance when phytic acid binds to hemoglobin A. With glycosylated hemoglobin there is no such change because the binding is blocked by the sugar moiety. Inter-assay CVs were 2-6% for both methods. In healthy subjects the mean (+/- SD) value for HbAl was about 1% higher with the spectrophotometric than the microcolumn method. For samples from 122 diabetics the correlation between values for HbAl obtained with the two methods was acceptable (r = 0.89), although the spectrophotometric technique yielded 2-4% higher values, a difference at least partly due to the absence of 2,3-diphosphoglycerate from the spectrophotometric standards. Adding 1.8 mmol of it per liter to these standards caused displacement of the standard curve; HbAl values then agreed well with those of the microcolumn method. The spectrophotometric procedure is easily automated, and therefore is well suited for large-scale analyses if problems with standards and calibration can be solved.

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Simple and Sensitive Spectrophotometric Method for Phytic Acid Determination in Grains

Food Analytical Methods ◽

10.1007/s12161-015-0387-0 ◽

2016 ◽

Vol 9 (7) ◽

pp. 2087-2096 ◽

Cited By ~ 13

Author(s):

Andréia Jacinto Agostinho ◽

Woodland de Souza Oliveira ◽

Daniela Santos Anunciação ◽

Josué Carinhanha Caldas Santos

Keyword(s):

Phytic Acid ◽

Spectrophotometric Method ◽

Sensitive Spectrophotometric Method ◽

Acid Determination

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Spectrophotometric Determination of Magnesium in Blood Serum Using Magon

Clinical Chemistry ◽

10.1093/clinchem/10.11.1028 ◽

1964 ◽

Vol 10 (11) ◽

pp. 1028-1038 ◽

Cited By ~ 8

Author(s):

Patricia J Burcar ◽

Albert J Boyle ◽

Robert E Mosher

Keyword(s):

Blood Serum ◽

Spectrophotometric Determination ◽

Perchloric Acid ◽

Spectrophotometric Method ◽

Serum Magnesium ◽

Aluminum Phosphate ◽

Acid Digestion ◽

Colored Complex ◽

Alcoholic Medium

Abstract A spectrophotometric method for the determination of serum magnesium using Magon, 1-azo-2-hydroxy-3- (2,4-dimethylcarboxanilido) naphthalene- 1’- (2-hydroxybenzene), is presented. The serum is prepared by a nitric-perchloric acid digestion. Interfering phosphate is removed as aluminum phosphate. The colored complex is developed at pH 11.2 in an alcoholic medium and read at 505 mµ.

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