Several herbal formulation were not properly documented due to poor scientific data as well as poor standard regulation in preparation and marketing. The study evaluate the effect of aqueous extract of Polyherbal formulation on Macrophages’ phagocytic function and Humoral immune response in Mice A total of Sixteen 16 Mice was used. Group I received normal saline, Group II-IV received 500 mg, 1000 mg, and 1500 mg of Polyherbal formulation respectively for 21 days each. The animals were sensitized and challenged with Sheep red blood cells at day 14th and 19th of the treatment respectively. On day 21st all the animals were injected with 0.1 ml Indian ink for carbon clearance assay and blood sample was collected at 1 minute and 15 minutes of the injection. The phagocytic function of Macrophages and humoral immune response were determined spectrophotometrically and Hemagglutination assay respectively. The results show that the highest carbonic particle clearances index (K) median score (Median=0.0228), Macrophage phagocytose index (α) median score (Median=3.249), organ weight index (g/100g) mean score (M=0.06633) and Hemagglutination antibody titre median score (Median=32.00) was recorded by Group IV when compared with other groups. The carbonic particle clearance (K) (p=0.02), phagocytic index of Macrophage (α) (p=0.03), and organ weight index (g/100g) (p<0.0001) significantly increase with increase in the Polyherbal formulation concentration. Hemagglutination antibody titre against Sheep red blood cells (p=0.02) also significantly increased. The extract has potential immunostimulatory activity on both Macrophages’ phagocytic function and humoral immune response in mice thus could be useful in improving immune responses.
The ontogeny of the Humoral immune response toE. colivaccine and to sheep red blood cells in young chicks
