Efficacy of entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae against Thaumetopoea pityocampa Shiff. (Lepidoptera: Thaumatopoeidae)

Abstract Background The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is a noxious pest of cruciferous crops all over the world causing serious economic damage. Management of insect pest generally depends on chemical control; however, due to development of resistance against all types of insecticides, alternative approaches especially utilization of a microbial agent is inevitable. Results Potential of 2 entomopathogenic fungi (EPF), viz., Beauveria bassiana and Metarhizium anisopliae, was evaluated against 2nd and 3rd larval instars of P. xylostella by adopting leaf dip and direct spraying methods under laboratory conditions. Significant mortality rate was achieved by each fungus under adopted methodologies. However, B. bassiana was found to be more effective in both conditions than M. anisopliae. Highest mean corrected mortality (77.80%) was recorded, when spores of B. bassiana were sprayed on the 2nd instar larvae (LC50=1.78×104/ml) after the 6th day of treatment. Similarly, incase of M. anisopliae LC50 for the 2nd instar at the same methodology was 2.78×104/ml with a mortality percentage of 70.0%. Offspring sex ratio was non-significantly related to treatment concentration and methodology, except for the control. Conclusion Beauveria bassiana and M. anisopliae had potential to suppress P. xylostella infestations when applied appropriately. Present findings suggested that B. bassiana and M. anisopliae when sprayed on immatures of host insect had more effect as compared to leaf dip procedure. Furthermore, no significant effect of concentrations was observed on sex ratio.

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Ecology of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae in temperate agroecosystems: Potential for conservation biological control

Biological Control ◽

10.1016/j.biocontrol.2007.07.007 ◽

2007 ◽

Vol 43 (2) ◽

pp. 145-155 ◽

Cited By ~ 267

Author(s):

Nicolai V. Meyling ◽

Jørgen Eilenberg

Keyword(s):

Biological Control ◽

Beauveria Bassiana ◽

Entomopathogenic Fungi ◽

Metarhizium Anisopliae ◽

Conservation Biological Control

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Evaluating the virulence of Metarhizium anisopliae (Deuteromycotina: Hyphomycetes) and Beauveria bassiana (Ascomycota: Hypocreales) isolates to Arabian rhinoceros beetle, Oryctes agamemnon arabicus

Journal of Entomological and Acarological Research ◽

10.4081/jear.2015.5180 ◽

2015 ◽

Vol 47 (3) ◽

pp. 117 ◽

Cited By ~ 1

Author(s):

M.W. Khudhair ◽

M.Z. Khalaf ◽

H.F. Alrubeai ◽

A.K. Shbar ◽

B.S. Hamad ◽

...

Keyword(s):

Mortality Rate ◽

Beauveria Bassiana ◽

Entomopathogenic Fungi ◽

Metarhizium Anisopliae ◽

Pupal Stage ◽

Control Treatment ◽

Rhinoceros Beetle ◽

Oryctes Agamemnon ◽

Lethal Time ◽

Short Time

Virulence of entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana were tested against Arabian Rhinoceros Beetle, Oryctes agamemnon arabicus larvae. Four concentrations (1×105, 1×107, 1×109 and 1×1011 conidia/mL–1) of two locally isolated entomopathogenic fungi spore suspensions were used in this study via larval direct spraying. Results revealed that both isolates can cause high mortality rate reaching 100% after 29 days. However, Beauveria bassiana scored higher mortality rate in short time especially at the concentration of 1×1011 conidia/ mL–1 with lethal time (LT)50 12.75 and LT90 20.00; while, Metarhizium anisopliae caused the higher percentage of malformed adults. Moreover, both isolates affected insect’s life cycle particularly in the pupal stage which was reduced remarkably by almost 50% in comparison with the control treatment.

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Effect of Biofertilizers and Neem Oil on the Entomopathogenic Fungi Beauveria bassiana (Bals.) Vuill. and Metarhizium anisopliae (Metsch.) Sorok

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132001000400013 ◽

2001 ◽

Vol 44 (4) ◽

pp. 419-423 ◽

Cited By ~ 29

Author(s):

Edson Hirose ◽

Pedro M. O. J. Neves ◽

João A. C. Zequi ◽

Luís H. Martins ◽

Cristiane H. Peralta ◽

...

Keyword(s):

Beauveria Bassiana ◽

Entomopathogenic Fungi ◽

Azadirachta Indica ◽

Organic Agriculture ◽

Metarhizium Anisopliae ◽

Neem Oil ◽

Colony Diameter ◽

Negative Effect ◽

Fungitoxic Effect

The in vitro fungitoxic effect of three biofertilizers, E.M.-4, Multibion Ô and Supermagro used in organic agriculture and the neem oil (Azadirachta indica A. Juss) on the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana was studied. These products were mixed in a medium where the two fungi were inoculated, and germination, vegetative growth and conidiogenesis were assessed. The biofertilizers Supermagro and E.M.- 4 showed to be less toxic for the two fungi whereas MultibionÔ caused major inhibition on M. anisopliae, with reductions in germination (-37.74%), colony diameter (-30.26%) and conidiogenesis (-42.62%). Neem oil promoted a larger negative effect on B. bassiana, inhibiting germination (-45.27%), colony diameter (-36.62%) and conidiogenesis (-84.93%).

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Effectiveness of Selected Entomopathogenic Fungi in Packed Rice Grain at Room Temperature Against Corcyra Cephalonica Stainton

ASEAN Journal on Science and Technology for Development ◽

10.29037/ajstd.103 ◽

2017 ◽

Vol 23 (3) ◽

pp. 183 ◽

Cited By ~ 6

Author(s):

Hendrawan Samodra ◽

Yusof Bin Ibrahim

Keyword(s):

Beauveria Bassiana ◽

High Mortality ◽

Entomopathogenic Fungi ◽

Metarhizium Anisopliae ◽

Room Temperature ◽

Larval Mortality ◽

Corcyra Cephalonica ◽

Rice Grain ◽

Rice Moth ◽

Tapioca Flour

Eight isolates of entomopathogenic fungi were evaluated as dried conidia against the rice moth, Corcyra cephalonica. In bioassays two isolates of Beauveria bassiana (BbGc and BbPs) and one isolate of Metarhizium anisopliae (MaPs) consistently gave high mortality to C. cephalonica larvae. Formulations in either kaolin, talc or tapioca flour (20 % w/w a.i.) thoroughly mixed with long grain rice in plastic cups (8 cm diameter by 5 cm) gave complete larval mortality by the 12th day of treatment. However, in general those formulated in kaolin and talc were more efficacious and faster to kill compared to those formulated in tapioca flour or the unformulated control. Even at the lowest rate of 0.05 g BbGc in kaolin provided 100% mortality 7 days after introduction compared with other dust formulations. Isolate BbGc in kaolin and talc administered at 0.4 g a.i. in 200 g rice packed in plastic kept at room temperature provided protection against the rice moth up to 4 months of storage. Larval mortality in excess of 90% was obtained 15 days after introduction. Formulations of MaPs was effective only within the first month of storage beyond which infectivity rapidly declined.

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Evaluation of the Entomopathogenic Fungi Beauveria bassiana, Metarhizium anisopliae, and Isaria fumosorosea for Control of Sitophilus oryzae

Journal of Food Protection ◽

10.4315/0362-028x.jfp-13-196 ◽

2014 ◽

Vol 77 (1) ◽

pp. 87-93 ◽

Cited By ~ 10

Author(s):

NICKOLAS G. KAVALLIERATOS ◽

CHRISTOS G. ATHANASSIOU ◽

MARIA M. AOUNTALA ◽

DEMETRIUS C. KONTODIMAS

Keyword(s):

Beauveria Bassiana ◽

Entomopathogenic Fungi ◽

Metarhizium Anisopliae ◽

Galleria Mellonella ◽

Sitophilus Oryzae ◽

Exposure Period ◽

Isaria Fumosorosea ◽

Stored Grain ◽

Stored Grain Pest ◽

Petri Dishes

The entomopathogenic fungi Beauveria bassiana, Metarhizium anisopliae, and Isaria fumosorosea were tested against the stored-grain pest Sitophilus oryzae. The fungi were isolated from the soil (from three locations in Attica, Greece: B. bassiana from Tatoion, M. anisopliae from Marathon, and I. fumosorosea from Aghios Stefanos) using larvae of Galleria mellonella as bait. Suspensions of 2.11 × 107 and 2.11 × 108, 1.77 × 107 and 1.77 × 108, and 1.81 × 107 and 1.81 × 108 conidia per ml of B. bassiana, M. anisopliae, and I. fumosorosea, respectively, were applied by three treatments: (i) sprayed on food and set in petri dishes with adults of S. oryzae, (ii) sprayed on adults of S. oryzae and set in petri dishes without food, and (iii) sprayed on adults of S. oryzae and set in petri dishes with food. The observed mortality of S. oryzae adults during the overall exposure period for the lowest, as well as for the highest, concentrations of B. bassiana, M. anisopliae, and I. fumosorosea ranged from 0 to 100%. Concentration was, in most of the cases tested, a critical parameter that determined the “speed of kill” of the exposed insect species for B. bassiana and M. anisopliae. Conversely, concentration was not that critical for I. fumosorosea, and survival was high in some of the combinations tested, even after 14 days of exposure. Both in the highest and the lowest concentrations of fungi, the mortality of S. oryzae adults was higher when the fungi were applied on adults than when they were applied on food. Higher mortality was observed when food was absent than when food was present, in most of the cases tested. The high efficacy levels recorded in the current study indicate that the tested fungi could be effective biocontrol agents against S. oryzae.

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