Granulated colloidal silicon dioxide-based self-microemulsifying tablets, as a versatile approach in enhancement of solubility and therapeutic potential of anti-diabetic agent: formulation design and in vitro/in vivo evaluation

2017 ◽  
Vol 43 (6) ◽  
pp. 1023-1032 ◽  
Author(s):  
Vikas Pandey ◽  
Ritu M. Gilhotra ◽  
Seema Kohli
Keyword(s):  
Silicon Dioxide ◽  
Therapeutic Potential ◽  
In Vivo Evaluation ◽  
Formulation Design

scholarly journals Nanogels of Succinylated Glycol Chitosan-Succinyl Prednisolone Conjugate: Preparation, In Vitro Characteristics and Therapeutic Potential

Pharmaceutics ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 333 ◽  
Author(s):  
Zhou ◽  
Ichikawa ◽  
Ikeuchi-Takahashi ◽  
Hattori ◽  
Onishi
Keyword(s):  
Therapeutic Potential ◽  
Trinitrobenzene Sulfonic Acid ◽  
Glycol Chitosan ◽  
In Vivo Evaluation ◽  
Drug Effectiveness ◽  
Thymus Atrophy ◽  
Negative Surface ◽  
Negative Surface Charge

A novel anionic nanogel system was prepared using succinylated glycol chitosan-succinyl prednisolone conjugate (S-GCh-SP). The nanogel, named NG(S), was evaluated in vitro and in vivo. S-GCh-SP formed a nanogel via the aggregation of hydrophobic prednisolone (PD) moieties and the introduced succinyl groups contributed to the negative surface charge of the nanogel. The resultant NG(S) had a PD content of 13.7% (w/w), was ca. 400 nm in size and had a -potential of −28 mV. NG(S) released PD very slowly at gastric pH and faster but gradually at small intestinal pH. Although NG(S) was easily taken up by the macrophage-like cell line Raw 264.7, it did not decrease cell viability, suggesting that the toxicity of the nanogel was very low. The in vivo evaluation was performed using rats with trinitrobenzene sulfonic acid (TNBS)-induced colitis. NG(S) and PD alone were not very effective at 5 mg PD eq./kg. However, NG(S) at 10 mg PD eq./kg markedly suppressed colonic damage, whereas PD alone did not. Furthermore, thymus atrophy was less with NG(S) than with PD alone. These results demonstrated that NG(S) is very safe, promotes drug effectiveness and has low toxicity. NG(S) has potential as a drug delivery system for the treatment of ulcerative colitis.

The Therapeutic Potential of Anti-HER2 2Rs15d Nanobody Labeled with 225Ac – an In Vitro and In Vivo Evaluation

2019 ◽  
Vol 50 (1) ◽  
pp. S7
Author(s):  
Marek Pruszynski ◽  
Matthias D’huyvetter ◽  
Magdalena Rodak ◽  
Yana Dekempeneer ◽  
Edyta Cedrowska ◽  
...  
Keyword(s):  
Therapeutic Potential ◽  
In Vivo Evaluation

The Therapeutic Potential of Anti-HER2 2Rs15d Nanobody Labeled with 225Ac – an In Vitro and In Vivo Evaluation

2019 ◽  
Vol 50 (4) ◽  
pp. S73-S74 ◽  
Author(s):  
Marek Pruszynski ◽  
Matthias D’huyvetter ◽  
Magdalena Rodak ◽  
Yana Dekempeneer ◽  
Edyta Cedrowska ◽  
...  
Keyword(s):  
Therapeutic Potential ◽  
In Vivo Evaluation

In vitro studies on anti-inflammatory, antioxidant and antihyperglycemic activities of potential probiotic Pediococcus acidilactici NCDC 252

2021 ◽  
Vol 16 (12) ◽  
pp. 18-23
Author(s):  
Shweta Dhanda ◽  
Praveen Kumar ◽  
Manjusha Choudhary ◽  
Suman Dhanda
Keyword(s):  
Protein Denaturation ◽  
Therapeutic Potential ◽  
Alpha Amylase ◽  
Fermented Foods ◽  
Pediococcus Acidilactici ◽  
Membrane Stabilization ◽  
In Vivo Evaluation ◽  
Anti Inflammatory

Probiotics are live microbes which positively influence the health when consumed in adequate amount. Lactic acid bacteria (LAB) are commonly used probiotics and are generally found in yogurt and fermented foods. They provide barrier for pathogens by secreting peptides and other metabolites. Pediococcus acidilactici NCDC 252 is a LAB of dairy origin with probiotic attributes. NCDC 252 was studied for in vitro anti-inflammatory, antioxidant and antihyperglycemic activities. Anti-inflammatory activity was studied by human red blood cell (HRBC) membrane stabilization method, protein (albumin) denaturation inhibitory activity and heat induced haemolysis. Antioxidant activity was evaluated by α, α-diphenyl-β- picrylhydrazyl (DPPH) radical and hydrogen peroxide (H2O2) scavenging assays. Alpha amylase inhibition assay was performed to examine antihyperglycemic effect. NCDC 252 exhibited potent anti-inflammatory but moderate antioxidant and antihyperglycemic activities as compared to control. NCDC 252 exhibited 65%, 70% and 49% membrane stabilization, protein denaturation and heat induced activity respectively. Scavenging effect was 45 % and 60% in H2O2 and DPPH assays respectively. Alpha amylase inhibition was 48 %. These results suggest therapeutic potential of NCDC 252 and open new avenues to treat disorders related to free radical generation such as inflammation and diabetes mellitus after in vivo evaluation of NCDC 252 to confirm its efficacy in animals.

scholarly journals In Vitro and In Vivo Evaluation of Three Newly Isolated Bacteriophage Candidates, phiEF7H, phiEF14H1, phiEF19G, for Treatment of Enterococcus faecalis Endophthalmitis

2021 ◽  
Vol 9 (2) ◽  
pp. 212
Author(s):  
Tatsuma Kishimoto ◽  
Waka Ishida ◽  
Tadahiro Nasukawa ◽  
Takako Ujihara ◽  
Isana Nakajima ◽  
...  
Keyword(s):  
Vision Loss ◽  
Therapeutic Potential ◽  
Vitreous Body ◽  
Bacteriophage Therapy ◽  
In Vivo Evaluation ◽  
Intravitreous Injection ◽  
Viable Bacteria ◽  
Vancomycin Resistant

Post-operative endophthalmitis caused by Enterococcus spp. progresses rapidly and often results in substantial and irreversible vision loss. Therefore, novel alternative treatments that are effective against enterococcal endophthalmitis are required. Bacteriophage therapy has the potential to be an optional therapy for infectious diseases. Therefore, we investigated the therapeutic potential of three newly isolated enterococcal phages, phiEF7H, phiEF14H1, and phiEF19G, in E. faecalis-induced endophthalmitis. These phages could lyse the broad-range E. faecalis, including strains derived from endophthalmitis and vancomycin-resistant E. faecalis in vitro, as determined by the streak test. Morphological and genomic analyses revealed that these phages were classified into the Herelleviridae genus Kochikohdavirus. The whole genomes of these phages contained 143,399, 143,280, and 143,400 bp, respectively. Endophthalmitis was induced in mice by injection of three strains of E. faecalis derived from post-operative endophthalmitis or vancomycin-resistant strains into the vitreous body. The number of viable bacteria and infiltration of neutrophils in the eye were both decreased by intravitreous injection of phiEF7H, phiEF14H1, and phiEF19G 6 h after injection of all E. faecalis strains. Thus, these results suggest that these newly isolated phages may serve as promising candidates for phage therapy against endophthalmitis.

In vitro and in vivo evaluation of the anti-inflammatory effects of Arzanol from Helichrysum italicum

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
J Bauer ◽  
F Dehm ◽  
A Koeberle ◽  
F Pollastro ◽  
G Appendino ◽  
...  
Keyword(s):  
In Vivo Evaluation ◽  
Anti Inflammatory
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