Biofuels based on vegetable oils offer the advantage being a sustainable and environmentally attractive alternative to conventional petroleum based fuel. The key issue in using vegetable oil-based fuels is oxidation stability, stoichiometric point, bio-fuel composition, antioxidants on the degradation and much oxygen with comparing to diesel gas oil. This provides a critical review of current understanding of main factor in storage method which affecting the biodiesel properties and characteristics. In the quest for fulfill the industry specifications standard; the fuel should be stored in a clean, dry and dark environment. Water and sediment contamination are basically housekeeping issues for biodiesel. Degradation by oxidation yields products that may compromise fuel properties, impair fuel quality and engine performance. The effect of storage method on the fuel properties and burning process in biodiesel fuel combustion will strongly affects the exhaust emissions.