Simultaneous detection of five biothreat agents in powder samples by a multiplexed suspension array

We have developed novel Bio-Plex assays for simultaneous detection ofBacillus anthracis, Yersinia pestis, Brucella spp., Francisella tularensis,andBurkholderia pseudomallei. Universal primers were used to amplify highly conserved region located within the 16S rRNA amplicon, followed by hybridized to pathogen-specific probes for identification of these five organisms. The other assay is based on multiplex PCR to simultaneously amplify five species-specific pathogen identification-targeted regions unique to individual pathogen. Both of the two arrays are validated to be flexible and sensitive for simultaneous detection of bioterrorism bacteria. However, universal primer PCR-based array could not identifyBacillus anthracis, Yersinia pestis,andBrucella spp.at the species level because of the high conservation of 16S rDNA of the same genus. The two suspension arrays can be utilized to detectBacillus anthracissterne spore andYersinia pestisEV76 from mimic “write powder” samples, they also proved that the suspension array system will be valuable tools for diagnosis of bacterial biothreat agents in environmental samples.

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Simultaneous detection of five antibiotics in milk by high-throughput suspension array technology

Talanta ◽

10.1016/j.talanta.2011.05.040 ◽

2011 ◽

Vol 85 (2) ◽

pp. 1160-1165 ◽

Cited By ~ 36

Author(s):

Pu Su ◽

Nan Liu ◽

Maoxiang Zhu ◽

Baoan Ning ◽

Ming Liu ◽

...

Keyword(s):

High Throughput ◽

Simultaneous Detection ◽

Suspension Array ◽

Array Technology

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Simultaneous Detection of Ochratoxin A and Fumonisin B1 in Cereal Samples Using an Aptamer–Photonic Crystal Encoded Suspension Array

Analytical Chemistry ◽

10.1021/ac503355n ◽

2014 ◽

Vol 86 (23) ◽

pp. 11797-11802 ◽

Cited By ~ 50

Author(s):

Sun Yue ◽

Xu Jie ◽

Li Wei ◽

Cao Bin ◽

Wang Dou Dou ◽

...

Keyword(s):

Photonic Crystal ◽

Ochratoxin A ◽

Simultaneous Detection ◽

Fumonisin B1 ◽

Suspension Array ◽

Cereal Samples

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Exploring target-specific primer extension in combination with a bead-based suspension array for multiplexed detection and typing using Streptococcus suis as a model pathogen

Journal of Veterinary Diagnostic Investigation ◽

10.1177/1040638717730384 ◽

2017 ◽

Vol 30 (1) ◽

pp. 71-77

Author(s):

Fimme J. van der Wal ◽

René P. Achterberg ◽

Conny van Solt-Smits ◽

Jan H. W. Bergervoet ◽

Marjanne de Weerdt ◽

...

Keyword(s):

Simultaneous Detection ◽

Sampling Strategy ◽

Streptococcus Suis ◽

Primer Extension ◽

Conventional Pcr ◽

Specific Primer ◽

Multiplexed Detection ◽

Suspension Array ◽

Protein Factor ◽

Pcr Assays

We investigated the feasibility of an assay based on target-specific primer extension, combined with a suspension array, for the multiplexed detection and typing of a veterinary pathogen in animal samples, using Streptococcus suis as a model pathogen. A procedure was established for simultaneous detection of 6 S. suis targets in pig tonsil samples (i.e., 4 genes associated with serotype 1, 2, 7, or 9, the generic S. suis glutamate dehydrogenase gene [ gdh], and the gene encoding the extracellular protein factor [ epf]). The procedure was set up as a combination of protocols: DNA isolation from porcine tonsils, a multiplex PCR, a multiplex target-specific primer extension, and finally a suspension array as the readout. The resulting assay was compared with a panel of conventional PCR assays. The proposed multiplex assay can correctly identify the serotype of isolates and is capable of simultaneous detection of multiple targets in porcine tonsillar samples. The assay is not as sensitive as the current conventional PCR assays, but with the correct sampling strategy, the assay can be useful for screening pig herds to establish which S. suis serotypes are circulating in a pig population.

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Simultaneous detection of platelet-specific antibodies based on a photonic crystal-encoded suspension array

Clinica Chimica Acta ◽

10.1016/j.cca.2016.04.031 ◽

2016 ◽

Vol 458 ◽

pp. 72-77 ◽

Cited By ~ 3

Author(s):

Xin Kong ◽

Baofen Ye ◽

Zixue Yang ◽

Baoan Chen ◽

Yun Ling

Keyword(s):

Photonic Crystal ◽

Simultaneous Detection ◽

Specific Antibodies ◽

Suspension Array

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Quantification of malaria antigens PfHRP2 and pLDH by quantitative suspension array technology in whole blood, dried blood spot and plasma

10.1101/730499 ◽

2019 ◽

Author(s):

Xavier Martiáñez-Vendrell ◽

Alfons Jiménez ◽

Ana Vásquez ◽

Ana Campillo ◽

Sandra Incardona ◽

...

Keyword(s):

Whole Blood ◽

Point Of Care ◽

External Validation ◽

Simultaneous Detection ◽

Parasite Clearance ◽

Dried Blood Spots ◽

Clinical Samples ◽

Lower Sensitivity ◽

Suspension Array ◽

Array Technology

ABSTRACTBackgroundMalaria diagnostics by rapid diagnostic tests (RDTs) relies primarily on the qualitative detection of Plasmodium falciparum histidine-rich protein 2 (PfHRP2) and Plasmodium sp lactate dehydrogenase (pLDH). As novel RDTs with increased sensitivity are being developed and implemented as point of care diagnostics, highly sensitive laboratory based assays are needed for evaluating RDTs performance. Here, a quantitative suspension array technology (qSAT) was developed, validated and applied for the simultaneous detection of PfHRP2 and pLDH in a variety of clinical samples (whole blood, plasma and dried blood spots) from different endemic countries.ResultsThe qSAT was specific for the target antigens, with analytical ranges of 6.8 to 762.8 pg/ml for PfHRP2 and 78.1 to 17076.6 pg/ml for P. falciparum (Pf-LDH). The assay detected P. vivax LDH (Pv-LDH) at a lower sensitivity than Pf-LDH (analytical range of 1093.20 to 187288.5 pg/ml). Both PfHRP2 and pLDH levels determined using the qSAT showed to positively correlate with parasite densities determined by quantitative PCR (Spearman r=0.59 and 0.75, respectively) as well as microscopy (Spearman r=0.40 and 0.75, respectively), suggesting the assay to be a good predictor of parasite density.ConclusionThis immunoassay can be used as a reference test for the detection and quantification of PfHRP2 and pLDH, and could serve for external validation of RDTs performance, to determine antigen persistence after parasite clearance, as well as a complementary tool to assess malaria burden in endemic settings.

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