Comparison of the transmembrane helices of bovine rhodopsin in the crystal structure and the C α template based on cryo-electron microscopy maps and sequence analysis of the G protein-coupled receptors

2002 ◽  
Vol 28 (8-9) ◽  
pp. 845-851 ◽  
Author(s):  
Siavoush Dastmalchi ◽  
Felix J. Kobus ◽  
Tiina P. Iismaa ◽  
Michael B. Morris ◽  
W. Bret Church
Keyword(s):  
Crystal Structure ◽  
Electron Microscopy ◽  
Sequence Analysis ◽  
G Protein ◽  
G Protein Coupled Receptors ◽  
Transmembrane Helices ◽  
Cryo Electron Microscopy ◽  
Bovine Rhodopsin ◽  
G Protein Coupled

scholarly journals Crystal structure of rhodopsin: a template for cone visual pigments and other G protein-coupled receptors

2002 ◽  
Vol 1565 (2) ◽  
pp. 168-182 ◽  
Author(s):  
R.E. Stenkamp ◽  
S. Filipek ◽  
C.A.G.G. Driessen ◽  
D.C. Teller ◽  
K. Palczewski
Keyword(s):  
Crystal Structure ◽  
G Protein ◽  
Visual Pigments ◽  
G Protein Coupled Receptors ◽  
G Protein Coupled

Cryo-EM: The Resolution Revolution and Drug Discovery

2020 ◽  
Vol 26 (1) ◽  
pp. 17-31
Author(s):  
Taiana Maia de Oliveira ◽  
Lotte van Beek ◽  
Fiona Shilliday ◽  
Judit É. Debreczeni ◽  
Chris Phillips
Keyword(s):  
Electron Microscopy ◽  
Drug Discovery ◽  
G Protein ◽  
Structural Biology ◽  
Single Particle ◽  
Trp Channels ◽  
G Protein Coupled Receptors ◽  
Imaging Analysis ◽  
Structure Based Drug Design ◽  
G Protein Coupled

Single-particle cryogenic electron microscopy (cryo-EM) has been elevated to the mainstream of structural biology propelled by technological advancements in numerous fronts, including imaging analysis and the development of direct electron detectors. The drug discovery field has watched with (initial) skepticism and wonder at the progression of the technique and how it revolutionized the molecular understanding of previously intractable targets. This article critically assesses how cryo-EM has impacted drug discovery in diverse therapeutic areas. Targets that have been brought into the realm of structure-based drug design by cryo-EM and are thus reviewed here include membrane proteins like the GABAA receptor, several TRP channels, and G protein-coupled receptors, and multiprotein complexes like the ribosomes, the proteasome, and eIF2B. We will describe these studies highlighting the achievements, challenges, and caveats.

scholarly journals First Principles Predictions of the Structure and Function of G-Protein-Coupled Receptors: Validation for Bovine Rhodopsin

2004 ◽  
Vol 86 (4) ◽  
pp. 1904-1921 ◽  
Author(s):  
Rene J. Trabanino ◽  
Spencer E. Hall ◽  
Nagarajan Vaidehi ◽  
Wely B. Floriano ◽  
Victor W.T. Kam ◽  
...  
Keyword(s):  
G Protein ◽  
First Principles ◽  
Structure And Function ◽  
G Protein Coupled Receptors ◽  
Bovine Rhodopsin ◽  
And Function ◽  
G Protein Coupled
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