ELISA and Chemiluminescent Enzyme Immunoassay for Sensitive and Specific Determination of Lead (II) in Water, Food and Feed Samples

Lead is a heavy metal with increasing public health concerns on its accumulation in the food chain and environment. Immunoassays for the quantitative measurement of environmental heavy metals offer numerous advantages over other traditional methods. ELISA and chemiluminescent enzyme immunoassay (CLEIA), based on the mAb we generated, were developed for the detection of lead (II). In total, 50% inhibitory concentrations (IC50) of lead (II) were 9.4 ng/mL (ELISA) and 1.4 ng/mL (CLEIA); the limits of detection (LOD) were 0.7 ng/mL (ic-ELISA) and 0.1 ng/mL (ic-CLEIA), respectively. Cross-reactivities of the mAb toward other metal ions were less than 0.943%, indicating that the obtained mAb has high sensitivity and specificity. The recovery rates were 82.1%–108.3% (ic-ELISA) and 80.1%–98.8% (ic-CLEIA), respectively. The developed methods are feasible for the determination of trace lead (II) in various samples with high sensitivity, specificity, fastness, simplicity and accuracy.

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Development of a phage chemiluminescent enzyme immunoassay with high sensitivity for the determination of imidaclothiz in agricultural and environmental samples

The Science of The Total Environment ◽

10.1016/j.scitotenv.2017.07.214 ◽

2017 ◽

Vol 609 ◽

pp. 854-860 ◽

Cited By ~ 9

Author(s):

Yuan Ding ◽

Xiude Hua ◽

Nana Sun ◽

Jiachuan Yang ◽

Jiaqi Deng ◽

...

Keyword(s):

Enzyme Immunoassay ◽

Environmental Samples ◽

High Sensitivity ◽

Chemiluminescent Enzyme Immunoassay

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A rapid and accurate method for screening T-2 toxin in food and feed using competitive AlphaLISA

FEMS Microbiology Letters ◽

10.1093/femsle/fnab029 ◽

2021 ◽

Vol 368 (6) ◽

Author(s):

Liwen Zhang ◽

Qingyu Lv ◽

Yuling Zheng ◽

Xuan Chen ◽

Decong Kong ◽

...

Keyword(s):

High Sensitivity ◽

Accurate Method ◽

Detection Methods ◽

Cereal Crops ◽

Detection Range ◽

Highly Sensitive ◽

Food And Feed ◽

Good Repeatability ◽

Feed Samples ◽

Better Than

ABSTRACT T-2 is a common mycotoxin contaminating cereal crops. Chronic consumption of food contaminated with T-2 toxin can lead to death, so simple and accurate detection methods in food and feed are necessary. In this paper, we establish a highly sensitive and accurate method for detecting T-2 toxin using AlphaLISA. The system consists of acceptor beads labeled with T-2-bovine serum albumin (BSA), streptavidin-labeled donor beads and biotinylated T-2 antibodies. T-2 in the sample matrix competes with T-2-BSA for antibodies. Adding biotinylated antibodies to the test well followed by T-2 and T-2-BSA acceptor beads yielded a detection range of 0.03–500 ng/mL. The half-maximal inhibitory concentration was 2.28 ng/mL and the coefficient of variation was <10%. In addition, this method had no cross-reaction with other related mycotoxins. This optimized method for extracting T-2 from food and feed samples achieved a recovery rate of approximately 90% in T-2 concentrations as low as 1 ng/mL, better than the performance of a commercial ELISA kit. This competitive AlphaLISA method offers high sensitivity, good specificity, good repeatability and simple operation for detecting T-2 toxin in food and feed.

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Determination of progesterone receptor by chemiluminescent enzyme immunoassay

Journal of Huazhong University of Science and Technology [Medical Sciences] ◽

10.1007/bf02829465 ◽

2003 ◽

Vol 23 (1) ◽

pp. 60-61

Author(s):

Yin Tiejun ◽

Gu Meijiao ◽

Zhou Yikai ◽

Zheng Wen ◽

Hu Wei ◽

...

Keyword(s):

Progesterone Receptor ◽

Enzyme Immunoassay ◽

Chemiluminescent Enzyme Immunoassay

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Amphenicols stability in medicated feed – development and validation of liquid chromatography method

Bulletin of the Veterinary Institute in Pulawy ◽

10.2478/bvip-2014-0095 ◽

2014 ◽

Vol 58 (4) ◽

pp. 621-629 ◽

Cited By ~ 4

Author(s):

Wojciech Jerzy Pietro ◽

Aneta Woźniak ◽

Katarzyna Pasik ◽

Wojciech Cybulski ◽

Dorota Krasucka

Keyword(s):

Liquid Chromatography ◽

Analytical Procedure ◽

Chromatography Method ◽

The Matrix ◽

The Mean ◽

Liquid Chromatography Method ◽

Sensitivity Specificity ◽

Development And Validation ◽

Feed Samples

Abstract A liquid chromatography-ultraviolet detection method for the determination of florfenicol (FF) and thiamphenicol (TAP) in feeds is presented. The method comprises the extraction of analytes from the matrix with a mixture of methanol and acetonitrile, drying of the extract, and its dissolution in phosphate buffer. The analysis was performed with a gradient programme of the mobile phase composed of acetonitrile and buffer (pH = 7.3) on a Zorbax Eclipse Plus C18 (150 × 4.6 mm, 5 μm) analytical column with UV (λ = 220 nm) detection. The analytical procedure has been successfully adopted and validated for quantitative determination of florfenicol and thiamphenicol in feed samples. Sensitivity, specificity, linearity, repeatability, and intralaboratory reproducibility were included in the validation. The mean recovery of amphenicols was 93.5% within the working range of 50-4000 mg/kg. Simultaneous determination of chloramphenicol, which is banned in the feed, was also included within the same procedure of FF and TAP stability studies. Storing the medicated feed at room temperature for up to one month decreased concentration in the investigated drugs even by 45%. These findings are relevant to successful provision of therapy to animals.

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Determination of Free Triiodothyronine by Chemiluminescent Enzyme Immunoassay

Chinese Journal of Analytical Chemistry ◽

10.1016/s1872-2040(08)60035-3 ◽

2008 ◽

Vol 36 (5) ◽

pp. 609-614 ◽

Cited By ~ 6

Author(s):

Zhen LIN ◽

Xu WANG ◽

Shi-Qi REN ◽

Guo-Nan CHEN ◽

Zhen-Jia LI ◽

...

Keyword(s):

Enzyme Immunoassay ◽

Free Triiodothyronine ◽

Chemiluminescent Enzyme Immunoassay

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Development of sensitive direct chemiluminescent enzyme immunoassay for the determination of dihydroartemisinin in plasma

Analytical and Bioanalytical Chemistry ◽

10.1007/s00216-015-8951-4 ◽

2015 ◽

Vol 407 (25) ◽

pp. 7823-7830 ◽

Cited By ~ 10

Author(s):

Laura Zehnacker ◽

Marie-Claire Nevers ◽

Véronique Sinou ◽

Dominique Parzy ◽

Christophe Créminon ◽

...

Keyword(s):

Enzyme Immunoassay ◽

Chemiluminescent Enzyme Immunoassay

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Comparison of Point-of-Care Ultrasonography and Radiography in the Diagnosis of Long-Bone Fractures

Medicina ◽

10.3390/medicina55070355 ◽

2019 ◽

Vol 55 (7) ◽

pp. 355 ◽

Cited By ~ 5

Author(s):

Avci ◽

Kozaci ◽

Tulubas ◽

Caliskan ◽

Yuksel ◽

...

Keyword(s):

Predictive Value ◽

Bone Fractures ◽

Point Of Care ◽

High Sensitivity ◽

Long Bone ◽

Imaging Method ◽

Fracture Characteristics ◽

Point Of Care Ultrasonography ◽

Sensitivity Specificity

Background and objectives: In this study, the accuracy of point-of-care ultrasonography (POCUS) was compared to radiography (XR) in the diagnosis of fractures, the determination of characteristics of the fractures, and treatment selection of fractures in patients admitted to the emergency department (ED) due to trauma and suspected long bone (LB) fractures. Materials and Methods: The patients were included in the study, who were admitted to ED due to trauma, and had physical examination findings suggesting the presence of fractures in LB (humerus, radius, ulna, femur, tibia, and fibula). The patients were evaluated by two emergency physicians (EP) in ED. The first EP examined LBs with POCUS and the second EP examined them with XR. LBs were evaluated on the anterior, posterior, medial, and lateral surfaces and from the proximal joint to the distal one (shoulder, elbow, wrist, hip, knee, and ankle joint) in both longitudinal and transverse axes with POCUS. Results: A total of 205 patients with suspected LB fractures were included in the study. LB fractures were determined in 99 patients with XR and in 105 patients with POCUS. The sensitivity, specificity, positive predictive value, negative predictive value of POCUS in determining the fractures were 99%, 93%, 93%, and 99%, respectively, compared to XR. Compared to XR, POCUS was able to determine 100% of fissure type fractures (kappa (κ) value: 0.765), 83% of linear fractures (κ: 0.848), 92% of fragmented fractures(κ: 0.756), 67% of spiral fractures (κ:0.798), 75% of avulsion type fractures (κ: 0.855), and 100% of full separation type fractures (κ: 0.855).Conclusions: This study has demonstrated that POCUS has a high sensitivity in diagnosing LB fractures. POCUS has a high sensitivity in identifying fracture characteristics. POCUS can be used as an alternative imaging method to XR in the diagnosis of LB fractures and in the determination of fracture characteristics.

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Quantitative analysis of chloramphenicol residues in shrimp muscle tissues by Chemiluminescent enzyme immunoassay

Czech Journal of Food Sciences ◽

10.17221/3399-cjfs ◽

2011 ◽

Vol 23 (No. 6) ◽

pp. 251-256

Author(s):

X. Chuanlai ◽

P. Cifang ◽

H. Kai ◽

J. Zhengyu ◽

W. Wukang

Keyword(s):

Enzyme Immunoassay ◽

Limit Of Detection ◽

Tween 20 ◽

Detection Range ◽

Muscle Tissues ◽

Physico Chemical ◽

Interassay Coefficient ◽

Chemiluminescent Enzyme Immunoassay ◽

Better Than

A competitive indirect chemiluminescent enzyme immunoassay (ic-CLEIA) has been developed for the determination of chloramphenicol (CAP) residues in shrimp. After the optimisation of four physico-chemical parameters, i.e. incubation time, concentration of Tween-20, concentration of PBS and its pH, the method developed gave a limit of detection of 0.01 ng/ml and a detection range from 0.03 ng/ml to 23.7 ng/ml, with an ED<sub>50</sub> of 0.47 ng/ml. The developed method has been validated on spiked shrimp samples in terms of precision (intra- and interassay coefficient variations of less than 10% and 15%, respectively), and of accuracy (mean recovery from 95% to 123%). All these parameters being better than those of the ELISA method which is widely used to detect chloramphenicol, it may be suggested that the CLEIA method can be used to detect aquatic samples instead of ELISA.  

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Liquid Chromatographic Determination of Fumonisins B1, B2, and B3 in Foods and Feeds

Journal of AOAC International ◽

10.1093/jaoac/75.2.313 ◽

1992 ◽

Vol 75 (2) ◽

pp. 313-318 ◽

Cited By ~ 76

Author(s):

Eric W Sydenham, ◽

Gordon S Shephard, ◽

Peter G Thiel

Keyword(s):

Chromatographic Method ◽

Chromatographic Determination ◽

Natural Occurrence ◽

Liquid Chromatographic Method ◽

Food And Feed ◽

Liquid Chromatographic Determination ◽

Liquid Chromatographic ◽

Feed Samples ◽

Initial Extraction

Abstract Three recently described and toxlcologlcally Important Fusarlum mycotoxins, fumonisln B1 (FB1), fumonisln B2 (FB2), and fumonisln B3 (FB3), are the major fumonisins produced In cultures of F. monlllforme, a fungus that occurs worldwide on corn. Contamination of food and feed with F. monlllforme has been associated with a number of diseases In both animals and humans. Aspects of a recently reported liquid chromatographic method for the determination of FB1 and FB2 in corn, including Initial extraction, extract purification, and stability of derivatives, were Investigated and, where necessary, optimized further both to reduce the analysis time and to include the co-determlnatlon of FB3. The method was applied for the determination of FB3, in a series of U.S. feed samples associated with outbreaks of equine leukoencephalomalacla, which were shown previously to contain both FBi and FB2. Twelve of the 13 feed samples contained FB3 at levels ranging between 50 and 2650 ng/g, corresponding to 2.2-18% of the total fumonisln concentrations present in the FB3-posltlve feed samples. This is the first report of the natural occurrence of FB3.

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