Localization of genes for lateral branch and female sex expression and construction of a molecular linkage map in cucumber (Cucumis sativus L.) with RAPD markers

2005 ◽  
Vol 15 (2) ◽  
pp. 143-148 ◽  
Author(s):  
Li Xiaozun ◽  
Pan Junsong ◽  
Wang Gang ◽  
Tian Libo ◽  
Si Longting ◽  
...  
HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 601f-602
Author(s):  
Felix C. Serquen ◽  
J. Bacher ◽  
J. Staub

Linkage maps in cucumber have been constructed in broad and narrow genetic base populations, using mostly RFLPs. RAPD markers are believed to be more advantageous than RFLPs for mapping in narrow crosses. An F3 population derived from F2 intercrossing cucumber inbred lines (G421 and H19) was used to construct a linkage map and to perform QTL analysis for horticultural traits recorded in two locations. One hundred three F3 families were used for mapping purpose. The parents were screened using ≈1500 primers yielding 80 RAPD markers that exhibited expected 3:1 Mendelian segregation. Additionally, female sex expression (F), little leaf (l), and determinate (de) loci also were evaluated in the segregating population. The linkage analysis and mapping was performed with MAPMAKER software, using a LOD score of 3.0 and recombination frequency of 0.40. QTL analysis was performed using one-way analysis of variance (ANOVA) and MAPMAKER/QTL. The linkage map integrates 83 map-points assembled into nine linkage groups. F and de loci mapped to linkage group `B', and the l locus was placed on linkage group `D'. The total map length is 628 cM, with an average distance between loci of 8.4 cM. Results from using one-way ANOVA and MAPMAKER/QTL had a good agreement for most QTL detected. Some QTL were location specific. Across locations four, one and three QTL were detected for sex expression, mainstem length, and number of laterals, respectively.


Euphytica ◽  
1986 ◽  
Vol 35 (3) ◽  
pp. 899-903 ◽  
Author(s):  
T. A. More ◽  
H. M. Munger

2010 ◽  
pp. 87-94 ◽  
Author(s):  
C.M. Liu ◽  
Y.S. Guo ◽  
R. Liu ◽  
B. Zhang ◽  
J.H. Xie ◽  
...  

Euphytica ◽  
2011 ◽  
Vol 182 (2) ◽  
pp. 167-176 ◽  
Author(s):  
Han Miao ◽  
Shengping Zhang ◽  
Xiaowu Wang ◽  
Zhonghua Zhang ◽  
Man Li ◽  
...  

2007 ◽  
Vol 6 (24) ◽  
pp. 2784-2791 ◽  
Author(s):  
A Yeboah Martin ◽  
Xuehao Chen ◽  
Rong Feng Chen ◽  
Liang Guohua ◽  
Gu Minghong

Genetika ◽  
2015 ◽  
Vol 47 (1) ◽  
pp. 349-356 ◽  
Author(s):  
Kalidas Pati ◽  
Das Munshi ◽  
Kanti Behera

The inheritance pattern of gynoecious sex expression in cucumber was studied by utilizing a gynoecious line (GBS-1) and two monoecious lines (Pusa Uday and Punjab Naveen). Crosses were made between gynoecious line (GBS-1) and monoecious lines (Pusa Uday and Punjab Naveen). The F1 and F2 population along with parental lines were evaluated to study the inheritance of this trait. All F1 hybrids showed gynoecious sex in both crosses and in the F2 generation, the observed distribution of plant phenotypes fitted the expected mendelian ratio of 3 (gynocious plant) : 1 (monoecious plant). The segregation of plant sex types suggested monogenic dominant control of gynoecious sex form in cucumber using genotype GBS-1.


Genetics ◽  
1993 ◽  
Vol 135 (4) ◽  
pp. 1175-1186
Author(s):  
R Weide ◽  
M F van Wordragen ◽  
R K Lankhorst ◽  
R Verkerk ◽  
C Hanhart ◽  
...  

Abstract In the past, a classical map of the tomato genome has been established that is based on linkage data from intraspecific Lycopersicon esculentum crosses. In addition, a high density molecular linkage map has recently been constructed using a L. esculentum x L. pennellii cross. As the respective maps only partially match, they provide limited information about the relative positions of classical and molecular markers. In this paper we describe the construction of an integrated linkage map of tomato chromosome 6 that shows the position of cDNA-, genomic DNA- and RAPD markers relative to 10 classical markers. Integration was achieved by using a L. esculentum line containing an introgressed chromosome 6 from L. pennellii in crosses to a variety of L. esculentum marker lines. In addition, an improved version of the classical linkage map is presented that is based on a combined analysis of new linkage data for 16 morphological markers and literature data. Unlike the classical map currently in use, the revised map reveals clustering of markers into three major groups around the yv, m-2 and c loci, respectively. Although crossing-over rates are clearly different when comparing intraspecific L. esculentum crosses with L. esculentum x L. pennellii crosses, the clusters of morphological markers on the classical map coincide with clusters of genomic- and cDNA-markers on the molecular map constructed by Tanksley and coworkers.


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