CONSTRUCTION OF A HIGH DENSITY MOLECULAR LINKAGE MAP FOR LYCHEE BASED ON AFLP AND RAPD MARKERS

2010 ◽  
pp. 87-94 ◽  
Author(s):  
C.M. Liu ◽  
Y.S. Guo ◽  
R. Liu ◽  
B. Zhang ◽  
J.H. Xie ◽  
...  
Keyword(s):  
Linkage Map ◽  
Rapd Markers ◽  
High Density ◽  
Molecular Linkage Map

Comparing the distribution of RAPD and RFLP markers in a high density linkage map of sugar beet

Genome ◽  
1997 ◽  
Vol 40 (5) ◽  
pp. 644-651 ◽  
Author(s):  
N.-O. Nilsson ◽  
C. Halldén ◽  
M. Hansen ◽  
A. Hjerdin ◽  
T. Säll
Keyword(s):  
Sugar Beet ◽  
Linkage Map ◽  
Rapd Markers ◽  
High Density ◽  
Rflp Markers ◽  
Linkage Phase ◽  
Mapping Procedure ◽  
Major Cluster ◽  
Distal Localization ◽  
High Density Linkage Map

The distribution of RAPD markers was compared with that of RFLP markers in a high density linkage map of sugar beet. The same mapping population of 161 F2 individuals was used to generate all the marker data. The total map comprises 160 RAPD and 248 RFLP markers covering 508 cM. Both the RAPD and the RFLP markers show a high degree of clustering over the nine linkage groups. The pattern is compatible with a strong distal localization of recombination in the sugar beet. It leads generally to one major cluster of markers in the centre of each linkage group. In regions of high marker density, dominant RAPD markers present in either linkage phase and codominant RFLP markers are subclustered relative to each other. This phenomenon is shown to be attributable to: (i) effects of the mapping procedure when dominant and codominant data are combined, (ii) effects of the mapping procedure when dominant data in both linkage phases are combined, and (iii) genuine differences in the way RAPD and RFLP markers are recruited.Key words: sugar beet, linkage map, RAPD, RFLP, clustering.

scholarly journals Integration of the classical and molecular linkage maps of tomato chromosome 6.

Genetics ◽  
1993 ◽  
Vol 135 (4) ◽  
pp. 1175-1186
Author(s):  
R Weide ◽  
M F van Wordragen ◽  
R K Lankhorst ◽  
R Verkerk ◽  
C Hanhart ◽  
...  
Keyword(s):  
Linkage Map ◽  
Rapd Markers ◽  
Linkage Data ◽  
Chromosome 6 ◽  
Morphological Markers ◽  
Limited Information ◽  
Tomato Genome ◽  
The Past ◽  
Molecular Linkage Map ◽  
Tomato Chromosome

Abstract In the past, a classical map of the tomato genome has been established that is based on linkage data from intraspecific Lycopersicon esculentum crosses. In addition, a high density molecular linkage map has recently been constructed using a L. esculentum x L. pennellii cross. As the respective maps only partially match, they provide limited information about the relative positions of classical and molecular markers. In this paper we describe the construction of an integrated linkage map of tomato chromosome 6 that shows the position of cDNA-, genomic DNA- and RAPD markers relative to 10 classical markers. Integration was achieved by using a L. esculentum line containing an introgressed chromosome 6 from L. pennellii in crosses to a variety of L. esculentum marker lines. In addition, an improved version of the classical linkage map is presented that is based on a combined analysis of new linkage data for 16 morphological markers and literature data. Unlike the classical map currently in use, the revised map reveals clustering of markers into three major groups around the yv, m-2 and c loci, respectively. Although crossing-over rates are clearly different when comparing intraspecific L. esculentum crosses with L. esculentum x L. pennellii crosses, the clusters of morphological markers on the classical map coincide with clusters of genomic- and cDNA-markers on the molecular map constructed by Tanksley and coworkers.

scholarly journals Mapping of Disease-Related Genes in Japanese Pear using a Molecular Linkage Map with RAPD Markers.

2001 ◽  
Vol 51 (3) ◽  
pp. 179-184 ◽  
Author(s):  
Hiroyuki Iketani ◽  
Kazuyuki Abe ◽  
Toshiya Yamamoto ◽  
Kazuo Kotobuki ◽  
Yoshihiko Sato ◽  
...  
Keyword(s):  
Linkage Map ◽  
Rapd Markers ◽  
Japanese Pear ◽  
Molecular Linkage Map ◽  
Disease Related Genes

scholarly journals Location of Four Morphological Markers (dgs, blu, arg, and y) in a Molecular Linkage Map of Common Bean

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 544B-544
Author(s):  
Gino Beltran ◽  
Geunhwa Jung ◽  
Mark Bassett ◽  
James Nienhuis
Keyword(s):  
Molecular Markers ◽  
Common Bean ◽  
Linkage Map ◽  
Morphological Traits ◽  
Rapd Markers ◽  
Genetic Relationships ◽  
Blue Flower ◽  
Recurrent Parent ◽  
Morphological Markers ◽  
Molecular Linkage Map

The development of a complete linkage map including both morphological and molecular markers is important to understand the genetic relationships among quantitatively and qualitatively inherited traits in common bean. The objective of this study was to identify RAPD markers linked to genes for four morphological traits using bulked segregant analysis and to map the markers to a molecular linkage map previously constructed in common bean. Three segregating populations were evaluated. Two BC2F2 populations with dgs (dark green savoy leaf) and blu (blue flower) induced mutant was developed with a Florida breeding line 7-1404 and 5-593 as the recurrent parent. In addition, a BC3F2 population with the y (yellow wax pod) and the arg (silvery green pod) mutants was developed from the Lamprecht line PI 527858 and 5-593 as the recurrent parent. RAPD markers linked in coupling to the morphological traits were detected to be 4.1 cM, 4.3 cM, 7.3 cM, and 12.3 cM distant from the dgs, blu, y, and arg genes, respectively. The linked RAPD markers were mapped in the molecular linkage map previously constructed using recombinant inbred population of the cross PC-50 × XAN-159. In this linkage map, we observed a linkage between the C locus and blu gene whose location was not previously known. In addition, a linkage between an abaxial leaf pubescent gene and dgs gene was observed. These results indicate that integrating morphological markers and molecular markers can result in a more complete genetic linkage map in common bean.

Mapping of Five Morphological Markers (dgs, blu, rnd, Ib, and asp) in a Molecular Linkage Map of Common Bean

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 547c-547
Author(s):  
Gino Beltran ◽  
Geunhwa Jung ◽  
Mark Bassett ◽  
James Nienhuis
Keyword(s):  
Common Bean ◽  
Linkage Map ◽  
Genetic Linkage ◽  
Rapd Markers ◽  
Genetic Linkage Map ◽  
Bulked Segregant Analysis ◽  
Blue Flower ◽  
Recurrent Parent ◽  
Molecular Linkage Map ◽  
Dark Green

The classical genetic linkage map of common bean contains only a fraction of the total genes that has been reported. These genes can be mapped in a molecular linkage map so that genetic relationship among diferent genes can be better understood. The objective of this study was to identify RAPD markers linked to genes for five morphological traits using bulked segregant analysis and to map the markers to a molecular linkage map previously constructed in common bean. Five segregating populations were evaluated. Three BC2F2 populations with dgs (dark green savoy leaf), blu (blue flower), and rnd (round leaf), respectively, were developed with a Florida breeding line 7-1404 and 5-593 as the recurrent parent. One BC3F2 population with the asp (dull seed coat) was developed from a BC2F2 5-593 line and 5-593 as the recurrent parent. Finally, an F2 segregating population for Ib (flat pod) was developed from `Hialeah' flat pod mutant × `Hialeah'. The linked RAPD markers were mapped in a molecular linkage map previously constructed using recombinant inbred population of the cross PC-50 × XAN-159. The results of this study indicate that integrating morphological and molecular merkers can result in a more complete genetic linkage map in common bean.

A high-density genetic linkage map of bronze loquat based on SSR and RAPD markers

2016 ◽  
Vol 12 (4) ◽  
Author(s):  
Shinji Fukuda ◽  
Keiichiro Ishimoto ◽  
Shusei Sato ◽  
Shingo Terakami ◽  
Naofumi Hiehata ◽  
...  
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Rapd Markers ◽  
Genetic Linkage Map ◽  
High Density

scholarly journals High-Density Genetic Map Construction and Identification of QTLs Controlling Leaf Abscission Trait in Poncirus trifoliata

2021 ◽  
Vol 22 (11) ◽  
pp. 5723
Author(s):  
Yuan-Yuan Xu ◽  
Sheng-Rui Liu ◽  
Zhi-Meng Gan ◽  
Ren-Fang Zeng ◽  
Jin-Zhi Zhang ◽  
...  
Keyword(s):  
Qtl Mapping ◽  
Linkage Map ◽  
Genetic Map ◽  
Expression Patterns ◽  
High Density ◽  
Poncirus Trifoliata ◽  
Leaf Abscission ◽  
Comparative Genomic ◽  
Map Comparison ◽  
Genomic Studies

A high-density genetic linkage map is essential for genetic and genomic studies including QTL mapping, genome assembly, and comparative genomic analysis. Here, we constructed a citrus high-density linkage map using SSR and SNP markers, which are evenly distributed across the citrus genome. The integrated linkage map contains 4163 markers with an average distance of 1.12 cM. The female and male linkage maps contain 1478 and 2976 markers with genetic lengths of 1093.90 cM and 1227.03 cM, respectively. Meanwhile, a genetic map comparison demonstrates that the linear order of common markers is highly conserved between the clementine mandarin and Poncirus trifoliata. Based on this high-density integrated citrus genetic map and two years of deciduous phenotypic data, two loci conferring leaf abscission phenotypic variation were detected on scaffold 1 (including 36 genes) and scaffold 8 (including 107 genes) using association analysis. Moreover, the expression patterns of 30 candidate genes were investigated under cold stress conditions because cold temperature is closely linked with the deciduous trait. The developed high-density genetic map will facilitate QTL mapping and genomic studies, and the localization of the leaf abscission deciduous trait will be valuable for understanding the mechanism of this deciduous trait and citrus breeding.

scholarly journals High-density linkage map construction and identification of loci regulating fruit quality traits in blueberry

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Molla F. Mengist ◽  
Hamed Bostan ◽  
Elisheba Young ◽  
Kristine L. Kay ◽  
Nicholas Gillitt ◽  
...  
Keyword(s):  
Linkage Map ◽  
Fruit Quality ◽  
Titratable Acidity ◽  
Fruit Weight ◽  
High Density ◽  
Soluble Solids ◽  
Quality Traits ◽  
Total Soluble Solids ◽  
Fruit Quality Traits ◽  
High Density Linkage Map

AbstractFruit quality traits play a significant role in consumer preferences and consumption in blueberry (Vaccinium corymbosum L). The objectives of this study were to construct a high-density linkage map and to identify the underlying genetic basis of fruit quality traits in blueberry. A total of 287 F1 individuals derived from a cross between two southern highbush blueberry cultivars, ‘Reveille’ and ‘Arlen’, were phenotyped over three years (2016–2018) for fruit quality-related traits, including titratable acidity, pH, total soluble solids, and fruit weight. A high-density linkage map was constructed using 17k single nucleotide polymorphisms markers. The linkage map spanned a total of 1397 cM with an average inter-loci distance of 0.08 cM. The quantitative trait loci interval mapping based on the hidden Markov model identified 18 loci for fruit quality traits, including seven loci for fruit weight, three loci for titratable acidity, five loci for pH, and three loci for total soluble solids. Ten of these loci were detected in more than one year. These loci explained phenotypic variance ranging from 7 to 28% for titratable acidity and total soluble solid, and 8–13% for pH. However, the loci identified for fruit weight did not explain more than 10% of the phenotypic variance. We also reported the association between fruit quality traits and metabolites detected by Proton nuclear magnetic resonance analysis directly responsible for these fruit quality traits. Organic acids, citric acid, and quinic acid were significantly (P < 0.05) and positively correlated with titratable acidity. Sugar molecules showed a strong and positive correlation with total soluble solids. Overall, the study dissected the genetic basis of fruit quality traits and established an association between these fruit quality traits and metabolites.

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