Laser micromachining has become a hotspot in recent years due to its high precision, non-contact and adjustable parameter. In this paper, TC4 titanium alloy implant samples were conducted to obtain specific surface textures through picosecond laser. The laser parameters which directly influenced the microstructure and characteristic of surface textures were optimized within the context of laser power, scanning speed and scanning number via response surface methodology. The microstructure was evaluated using scanning electron microscope (SEM) while the feature size of the surface textures was measured through surface 3D profiler. In addition, endothelial cell culture was conducted to investigate the biofunctionalization of samples with specific surface textures. It demonstrated that well-structured textures played an important role in promoting cell adhesion and proliferation for titanium alloy implants.