Simultaneous production of propionic acid and vitamin B12 from corn stalk hydrolysates by Propionibacterium freudenreichii in an expanded bed adsorption bioreactor

2020 ◽  
Vol 50 (8) ◽  
pp. 763-767
Author(s):  
Peng Wang ◽  
Chen Shen ◽  
Luwei Li ◽  
Jinfeng Guo ◽  
Qinqin Cong ◽  
...  
Keyword(s):  
Vitamin B12 ◽  
Propionic Acid ◽  
Expanded Bed Adsorption ◽  
Corn Stalk ◽  
Propionibacterium Freudenreichii ◽  
Expanded Bed ◽  
Simultaneous Production

Metabolic flux analysis of simultaneous production of vitamin B12 and propionic acid in a coupled fermentation process by Propionibacterium freudenreichii

2021 ◽  
Author(s):  
Yuhan Zhang ◽  
Xiaolian Li ◽  
Ziqiang Wang ◽  
Yunshan Wang ◽  
Yuanyuan Ma ◽  
...  
Keyword(s):  
Propionic Acid ◽  
Fermentation Process ◽  
Metabolic Flux ◽  
Flux Distribution ◽  
Vitamin B ◽  
Flux Analysis ◽  
Propionibacterium Freudenreichii ◽  
Simultaneous Production ◽  
Metabolic Flux Distribution ◽  
New Strategy

Abstract The metabolic processes involved in simultaneous production of vitamin B12 and propionic acid by Propionibacterium freudenreichii are very complicated. To further investigate the regulatory mechanisms of this metabolism, a simplified metabolic network was established. The effects of glucose feeding, propionic acid removal, and 5,6-dimethylbenzimidazole (DMB) addition on the metabolic flux distribution were investigated. The results showed that synthesis of propionic acid can be increased by increasing the metabolic flux through the oxaloacetate and methylmalonyl-CoA branches in the early and middle stages of the coupled fermentation. After DMB addition, the synthesis of vitamin B12 was significantly enhanced via increased metabolic flux through the δ-aminolevulinate branch, which promoted the synthesis of uroporphyrinogen III, a precursor of vitamin B12. Therefore, the analysis of metabolic flux at key nodes can provide theoretical guidance for the optimization of P. freudenreichii fermentation processes. In an experimental coupled fermentation process, the concentrations of vitamin B12 and propionic acid reached 21.6 and 50.12 g/L respectively, increased by 105.71% and 73.91% compared with batch fermentation, which provides a new strategy for industrial production.

scholarly journals Research on the ability of propionic acid and vitamin B12 biosynthesis by Propionibacterium freudenreichii strain T82

2017 ◽  
Vol 111 (6) ◽  
pp. 921-932 ◽  
Author(s):  
Kamil Piwowarek ◽  
Edyta Lipińska ◽  
Elżbieta Hać-Szymańczuk ◽  
Anna Bzducha-Wróbel ◽  
Alicja Synowiec
Keyword(s):  
Vitamin B12 ◽  
Propionic Acid ◽  
Propionibacterium Freudenreichii

Expanded bed adsorption as a fast technique for the large-scale purification of the complete isoform pool of Ber e 1, the major allergen from Brazil nuts

2006 ◽  
Vol 50 (3) ◽  
pp. 275-281 ◽  
Author(s):  
Evelien L. van Boxtel ◽  
Gerrit A. van Koningsveld ◽  
Stef J. Koppelman ◽  
Lambertus A. M. van den Broek ◽  
Alfons G. J. Voragen ◽  
...  
Keyword(s):  
Large Scale ◽  
Major Allergen ◽  
Expanded Bed Adsorption ◽  
Expanded Bed ◽  
Brazil Nuts
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