scholarly journals Metabolic Flux Analysis of Simultaneous Production of Vitamin B12 and Propionic Acid in a Coupled Fermentation Process by Propionibacterium freudenreichii

2021 ◽  
Author(s):  
Yuhan Zhang ◽  
Xiaolian Li ◽  
Ziqiang Wang ◽  
Yunshan Wang ◽  
Yuanyuan Ma ◽  
...  
Keyword(s):  
Vitamin B12 ◽  
Propionic Acid ◽  
Metabolic Flux Analysis ◽  
Fermentation Process ◽  
Metabolic Flux ◽  
Flux Analysis ◽  
Propionibacterium Freudenreichii ◽  
Simultaneous Production

Metabolic flux analysis of simultaneous production of vitamin B12 and propionic acid in a coupled fermentation process by Propionibacterium freudenreichii

2021 ◽  
Author(s):  
Yuhan Zhang ◽  
Xiaolian Li ◽  
Ziqiang Wang ◽  
Yunshan Wang ◽  
Yuanyuan Ma ◽  
...  
Keyword(s):  
Propionic Acid ◽  
Fermentation Process ◽  
Metabolic Flux ◽  
Flux Distribution ◽  
Vitamin B ◽  
Flux Analysis ◽  
Propionibacterium Freudenreichii ◽  
Simultaneous Production ◽  
Metabolic Flux Distribution ◽  
New Strategy

Abstract The metabolic processes involved in simultaneous production of vitamin B12 and propionic acid by Propionibacterium freudenreichii are very complicated. To further investigate the regulatory mechanisms of this metabolism, a simplified metabolic network was established. The effects of glucose feeding, propionic acid removal, and 5,6-dimethylbenzimidazole (DMB) addition on the metabolic flux distribution were investigated. The results showed that synthesis of propionic acid can be increased by increasing the metabolic flux through the oxaloacetate and methylmalonyl-CoA branches in the early and middle stages of the coupled fermentation. After DMB addition, the synthesis of vitamin B12 was significantly enhanced via increased metabolic flux through the δ-aminolevulinate branch, which promoted the synthesis of uroporphyrinogen III, a precursor of vitamin B12. Therefore, the analysis of metabolic flux at key nodes can provide theoretical guidance for the optimization of P. freudenreichii fermentation processes. In an experimental coupled fermentation process, the concentrations of vitamin B12 and propionic acid reached 21.6 and 50.12 g/L respectively, increased by 105.71% and 73.91% compared with batch fermentation, which provides a new strategy for industrial production.

Optimizing the Fermentation Process for Recombinant Escherichia coli on Base of Metabolic Flux Analysis of Amino Acids

2012 ◽  
Vol 550-553 ◽  
pp. 1055-1059
Author(s):  
Tao Zhang ◽  
Yan E Luo ◽  
Dai Di Fan ◽  
Lei Guo ◽  
Ting Zhen Mu
Keyword(s):  
Amino Acids ◽  
Cell Growth ◽  
Metabolic Flux Analysis ◽  
Fermentation Process ◽  
Metabolic Flux ◽  
Biological Activities ◽  
Basic Unit ◽  
Flux Analysis ◽  
Protein Molecules ◽  
Affect Cell Growth

Amino acids, the basic unit of protein molecules, are closely related to some important biological activities and they affect cell growth and metabolism directly or indirectly. Previous studies showed that adding amino acids can improve the productivity of human-like collagen (HLC). Thus, we analyzed the amino acids metabolism during the fermentation process. The results of metabolic flux analysis of amino acids implied that the cell growth and production of HLC were active as long as the free amino acids in the medium were adequate. This suggested control the concentration of amino acids could improve cell growth and human-like collagen production.

Sign in / Sign up

Export Citation Format

Share Document