scholarly journals Adaptation of glass columns for clean-up RP-HPLC determination of aflatoxins with post-column derivatisation with bromine and fluorescence detection.

2009 ◽  
Vol 9 (3) ◽  
Author(s):  
VO Owino
Keyword(s):  
Fluorescence Detection ◽  
Hplc Determination ◽  
Rp Hplc ◽  
Glass Columns

A new selective pre-column ninhydrin-based derivatization for a RP-HPLC determination of plasma asymmetric dimethyl-l-arginine (ADMA) by fluorescence detection

Amino Acids ◽  
2007 ◽  
Vol 34 (4) ◽  
pp. 677-682 ◽  
Author(s):  
Salvatore Sotgia ◽  
Angelo Zinellu ◽  
Gerard Aime Pinna ◽  
Luca Deiana ◽  
Ciriaco Carru
Keyword(s):  
Fluorescence Detection ◽  
Hplc Determination ◽  
Rp Hplc

A new method for the HPLC determination of gamma-hydroxybutyric acid (GHB) following derivatization with a coumarin analogue and fluorescence detection

Talanta ◽  
2008 ◽  
Vol 75 (2) ◽  
pp. 356-361 ◽  
Author(s):  
Constantinos K. Zacharis ◽  
Nikolaos Raikos ◽  
Nikolaos Giouvalakis ◽  
Helen Tsoukali-Papadopoulou ◽  
Georgios A. Theodoridis
Keyword(s):  
Fluorescence Detection ◽  
New Method ◽  
Hplc Determination ◽  
Hydroxybutyric Acid

scholarly journals HPLC determination of perfluorinated carboxylic acids with fluorescence detection

2010 ◽  
Vol 172 (3-4) ◽  
pp. 409-417 ◽  
Author(s):  
Ewa Poboży ◽  
Edyta Król ◽  
Lena Wójcik ◽  
Mariusz Wachowicz ◽  
Marek Trojanowicz
Keyword(s):  
Carboxylic Acids ◽  
Fluorescence Detection ◽  
Hplc Determination ◽  
Perfluorinated Carboxylic Acids

Method Validation for the Quantitative Analysis of Aflatoxins (B1, B2, G1, and G2) and Ochratoxin A in Processed Cereal-Based Foods by HPLC with Fluorescence Detection

2015 ◽  
Vol 98 (4) ◽  
pp. 939-945 ◽  
Author(s):  
Işil Gazioğlu ◽  
Ufuk Kolak
Keyword(s):  
Quantitative Analysis ◽  
Simultaneous Determination ◽  
Ochratoxin A ◽  
Fluorescence Detection ◽  
Aflatoxin B1 ◽  
Hplc Analysis ◽  
Extraction Procedure ◽  
Immunoaffinity Column ◽  
Rp Hplc

Abstract Modified AOAC 991.31 and AOAC 2000.03 methods for the simultaneous determination of total aflatoxins (AFs), aflatoxin B1, and ochratoxin A (OTA) in processed cereal-based foods by RP-HPLC coupled with fluorescence detection were validated. A KOBRA® Cell derivatization system was used to analyze total AFs. One of the modifications was the extraction procedure of mycotoxins. Both AFs and OTA were extracted with methanol–water (75 + 25, v/v) and purified with an immunoaffinity column before HPLC analysis. The modified methods were validated by measuring the specificity, selectivity, linearity, sensitivity, accuracy, repeatability, reproducibility, recovery, LOD, and LOQ parameters. The validated methods were successfully applied for the simultaneous determination of mycotoxins in 81 processed cereal-based foods purchased in Turkey. These rapid, sensitive, simple, and validated methods are suitable for the simultaneous determination of AFs and OTA in the processed cereal-based foods.

scholarly journals High-Performance Liquid Chromatographic Determination of Dihydroergocristine in a Pharmaceutical Formulation with Fluorescence Detection

2010 ◽  
Vol 93 (1) ◽  
pp. 97-101
Author(s):  
Michal Douša ◽  
Michaela Dubovská
Keyword(s):  
Fluorescence Detection ◽  
Mobile Phase ◽  
High Performance ◽  
Pharmaceutical Preparation ◽  
Potassium Dihydrogen Phosphate ◽  
Sample Pretreatment ◽  
Chromatographic Determination ◽  
Hplc Determination ◽  
Dihydrogen Phosphate

Abstract A rapid procedure based on a direct extraction and HPLC determination of dihydroergocristine in a pharmaceutical preparation with fluorescence detection has been developed and validated. The optimized chromatographic conditions included a Purospher RP18e column, 5 µm particle size, 250 4.0 mm, and 25 mM potassium dihydrogen phosphate buffer (pH 2.8)acetonitrile (60 + 40, v/v) mobile phase at a flow rate of 1 mL/min. The separation was carried out at 50C, and the injection volume was 5 L. Fluorescence detection was performed at an excitation and emission wavelength of 224 and 344 nm, respectively. The mobile phase parameters such as organic solvent composition, temperature, and pH were studied. The proposed method has the advantages of a very simple sample pretreatment and fast HPLC determination.

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