Ultra-performance liquid chromatography (UPLC) for the determination of organic acids – The intermediates of branched-chain amino acid biosynthesis in Escherichia coli strains

2020 ◽  
Vol 43 (19-20) ◽  
pp. 844-851
Author(s):  
Elizaveta N. Fedorova ◽  
Darya O. Varlamova ◽  
Alexandr D. Kivero ◽  
Kristina D. Guk ◽  
Leonid R. Ptitsyn
Keyword(s):  
Escherichia Coli ◽  
Liquid Chromatography ◽  
Amino Acid ◽  
Organic Acids ◽  
Ultra Performance Liquid Chromatography ◽  
Amino Acid Biosynthesis ◽  
Acid Biosynthesis ◽  
Branched Chain Amino Acid ◽  
Branched Chain

scholarly journals Homocysteine Toxicity in Escherichia coli Is Caused by a Perturbation of Branched-Chain Amino Acid Biosynthesis

2005 ◽  
Vol 187 (13) ◽  
pp. 4362-4371 ◽  
Author(s):  
Nina L. Tuite ◽  
Katy R. Fraser ◽  
Conor P. O'Byrne
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Biosynthetic Pathway ◽  
Amino Acid Biosynthesis ◽  
Inhibitory Effects ◽  
Acid Biosynthesis ◽  
Threonine Deaminase ◽  
E Coli ◽  
Branched Chain Amino Acid ◽  
Branched Chain

ABSTRACT In Escherichia coli the sulfur-containing amino acid homocysteine (Hcy) is the last intermediate on the methionine biosynthetic pathway. Supplementation of a glucose-based minimal medium with Hcy at concentrations greater than 0.2 mM causes the growth of E. coli Frag1 to be inhibited. Supplementation of Hcy-treated cultures with combinations of branched-chain amino acids containing isoleucine or with isoleucine alone reversed the inhibitory effects of Hcy on growth. The last intermediate of the isoleucine biosynthetic pathway, α-keto-β-methylvalerate, could also alleviate the growth inhibition caused by Hcy. Analysis of amino acid pools in Hcy-treated cells revealed that alanine, valine, and glutamate levels are depleted. Isoleucine could reverse the effects of Hcy on the cytoplasmic pools of valine and alanine. Supplementation of the culture medium with alanine gave partial relief from the inhibitory effects of Hcy. Enzyme assays revealed that the first step of the isoleucine biosynthetic pathway, catalyzed by threonine deaminase, was sensitive to inhibition by Hcy. The gene encoding threonine deaminase, ilvA, was found to be transcribed at higher levels in the presence of Hcy. Overexpression of the ilvA gene from a plasmid could overcome Hcy-mediated growth inhibition. Together, these data indicate that in E. coli Hcy toxicity is caused by a perturbation of branched-chain amino acid biosynthesis that is caused, at least in part, by the inhibition of threonine deaminase.

Regulation of branched-chain amino acid biosynthesis in Streptomyces fradiae, a producer of tylosin

1989 ◽  
Vol 151 (6) ◽  
pp. 537-540 ◽  
Author(s):  
Aleš Vančura ◽  
Ivana Vančurová ◽  
Jan Kopecký ◽  
Jaroslav Maršálek ◽  
Daniel Cikánek ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Biosynthesis ◽  
Streptomyces Fradiae ◽  
Acid Biosynthesis ◽  
Branched Chain Amino Acid ◽  
Branched Chain

Imazethapyr, an inhibitor of the branched-chain amino acid biosynthesis, induces aerobic fermentation in pea plants

2002 ◽  
Vol 114 (4) ◽  
pp. 524-532 ◽  
Author(s):  
Susana Gaston ◽  
Ana Zabalza ◽  
Esther M. González ◽  
Cesar Arrese-Igor ◽  
Pedro M. Aparicio-Tejo ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Biosynthesis ◽  
Acid Biosynthesis ◽  
Aerobic Fermentation ◽  
Branched Chain Amino Acid ◽  
Branched Chain

Herbicides inhibiting branched-chain amino acid biosynthesis

1990 ◽  
Vol 29 (3) ◽  
pp. 241-246 ◽  
Author(s):  
William K. Moberg ◽  
Barrington Cross
Keyword(s):  
Amino Acid ◽  
Amino Acid Biosynthesis ◽  
Acid Biosynthesis ◽  
Branched Chain Amino Acid ◽  
Branched Chain
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