Species diversity of woody recruits within Japanese cedar (Cryptomeria japonica) plantations established on grasslands: the effects of site conditions and landscape

2018 ◽  
Vol 23 (3) ◽  
pp. 156-165 ◽  
Author(s):  
Tetsuya Igarashi ◽  
Takashi Masaki
2013 ◽  
Vol 5 (3) ◽  
pp. 331
Author(s):  
Xie Ting-Ting ◽  
Su Pei-Xi ◽  
Zhou Zi-Juan ◽  
Li Shan-Jia ◽  
Zhang Hai-Na

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yoshihiko Nanasato ◽  
Masafumi Mikami ◽  
Norihiro Futamura ◽  
Masaki Endo ◽  
Mitsuru Nishiguchi ◽  
...  

AbstractCryptomeria japonica (Japanese cedar or sugi) is one of the most important coniferous tree species in Japan and breeding programs for this species have been launched since 1950s. Genome editing technology can be used to shorten the breeding period. In this study, we performed targeted mutagenesis using the CRISPR/Cas9 system in C. japonica. First, the CRISPR/Cas9 system was tested using green fluorescent protein (GFP)-expressing transgenic embryogenic tissue lines. Knock-out efficiency of GFP ranged from 3.1 to 41.4% depending on U6 promoters and target sequences. The GFP knock-out region was mottled in many lines, indicating genome editing in individual cells. However, in 101 of 102 mutated individuals (> 99%) from 6 GFP knock-out lines, embryos had a single mutation pattern. Next, we knocked out the endogenous C. japonica magnesium chelatase subunit I (CjChlI) gene using two guide RNA targets. Green, pale green, and albino phenotypes were obtained in the gene-edited cell lines. Sequence analysis revealed random deletions, insertions, and replacements in the target region. Thus, targeted mutagenesis using the CRISPR/Cas9 system can be used to modify the C. japonica genome.


2019 ◽  
Vol 7 (1) ◽  
Author(s):  
Furwoko Kirno ◽  
Dwi Astiani ◽  
Hanna Artuti Ekamawanti

The activities of the community around the peat swamp forest cause disruption of the ecosystem from the sustainability of the forest area's resources. Land clearing and peatland fires also affect the presence of ferns. The new environmental conditions due to changes in land cover cause ferns to be affected by these changes. Kuala Dua Village Kubu Raya Regency has secondary peat swamp forest and open peat land due to fires 3-4 years earlier so it is overgrown with a variety of new types of flora, one of which is a type of fern. The objective of the study is to describe species diversity of ferns and their site conditions in secondary peat swamp forests and open peatlands of Kuala Dua Village, Kubu Raya District. This research was conducted with survey method using multiple plot samples. The plots determination was done by purposive sampling which was taken place on the site where there were many species of ferns land. Three plots were on each land covered condition with a plot size of 5 m x 5 m that divided into 25 of a 1 m x 1 m sub plots. The results show that 5 species of ferns found in peat swamp forest and 4 species of ferns were found on open peatlands. The species that has the highest INP value in the forest was Asplenium belangeri and the species that has the INP value in open peatland was Nephrolefis falcata. Dominant Index, Spesies Diversity Index and Spesies Abudance Index were 0,12 and 0,18; 0,30 and 0,36; 0,51 and 0,52 on the forest and open peatland respectively. The Similarity Index value (IS) of ferns in secondary peat swamp forest and open peatland is 44%. The study shows dissimility of species fern in peat swamp forests and open peatlands.Keywords: Open peatland, Pteridophyta, secondary peat swamp forest


2015 ◽  
Vol 7 (3) ◽  
pp. 295 ◽  
Author(s):  
Jaechun Lee ◽  
Keun Hwa Lee ◽  
Hye-Sook Lee ◽  
Sung-Chul Hong ◽  
Jeong Hong Kim

2009 ◽  
Vol 96 (1-3) ◽  
pp. 163-175 ◽  
Author(s):  
Kazuya Nishina ◽  
Chisato Takenaka ◽  
Shigehiro Ishizuka

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