Diagnosis of COVID-19: hemagglutinin-esterase gene is used for molecular assays?

AbstractTo face the COVID-19 pandemic, the need for fast and reliable diagnostic assays for the detection of SARS-CoV-2 is immense. We describe our laboratory experiences evaluating nine commercially available real-time RT-PCR assays. We found that assays differed considerably in performance and validation before routine use is mandatory.

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Simpler and faster Covid-19 testing: Strategies to streamline SARS-CoV-2 molecular assays

EBioMedicine ◽

10.1016/j.ebiom.2021.103236 ◽

2021 ◽

Vol 64 ◽

pp. 103236

Author(s):

Nuttada Panpradist ◽

Qin Wang ◽

Parker S. Ruth ◽

Jack H. Kotnik ◽

Amy K. Oreskovic ◽

...

Keyword(s):

Molecular Assays ◽

Testing Strategies

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Comparison of Diagnostic Performance of Five Molecular Assays for Detection of SARS-CoV-2

Diagnostic Microbiology and Infectious Disease ◽

10.1016/j.diagmicrobio.2021.115518 ◽

2021 ◽

pp. 115518

Author(s):

Neena Kanwar ◽

Dithi Banerjee ◽

Anjana Sasidharan ◽

Ayah Abdulhamid ◽

Marissa Larson ◽

...

Keyword(s):

Diagnostic Performance ◽

Molecular Assays

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Structure and differential mechanisms of regulation of expression of a serine esterase gene in activated human T lymphocytes.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)68794-0 ◽

1988 ◽

Vol 263 (13) ◽

pp. 6363-6369

Author(s):

A Caputo ◽

D Fahey ◽

C Lloyd ◽

R Vozab ◽

E McCairns ◽

...

Keyword(s):

T Lymphocytes ◽

Serine Esterase ◽

Regulation Of Expression ◽

Human T Lymphocytes ◽

Esterase Gene

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The use of chemical and molecular microbial indicators for faecal source identification

Water Science & Technology ◽

10.2166/wst.2003.0155 ◽

2003 ◽

Vol 47 (3) ◽

pp. 39-43 ◽

Cited By ~ 33

Author(s):

B. Gilpin ◽

T. James ◽

F. Nourozi ◽

D. Saunders ◽

P. Scholes ◽

...

Keyword(s):

Direct Pcr ◽

Animal Group ◽

Faecal Pollution ◽

Molecular Assays ◽

Chemical Indicators ◽

Fluorescent Whitening Agents ◽

Feral Animals ◽

Oxidation Ponds ◽

Specific Species ◽

Faecal Sterols

Identifying the source of faecal pollution is important to enable appropriate management of faecal pollution of water. We are developing and evaluating a combination of these microbial and chemical indicators better able to identify the source of faecal pollution. These assays make use of a combination of direct PCR, culturing, and colony hybridisation to identify source specific species of Bifidobacterium, Rhodococcus and Bacteroides. In conjunction with assays for (a) fluorescent whitening agents and (b) faecal sterols and stanols, these indicators were able to identify human derived faecal pollution in river water containing inputs from septic tanks, municipal oxidation ponds, farmed animals and feral animals. Differentiating amongst the animal sources was more difficult and will require development of molecular assays for organisms specific to each animal group.

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Recent advances and novel approaches in laboratory-based diagnostic mycology

Medical Mycology ◽

10.1093/mmy/myy159 ◽

2019 ◽

Vol 57 (Supplement_3) ◽

pp. S259-S266 ◽

Cited By ~ 4

Author(s):

P Lewis White

Keyword(s):

Point Of Care ◽

Antibody Detection ◽

Molecular Testing ◽

Point Of Care Testing ◽

Proximity Ligation ◽

Molecular Assays ◽

Lateral Flow Assays ◽

Novel Approaches ◽

Exciting Time ◽

Generation Sequencing

Abstract The field of diagnostic mycology represents much more than culture and microscopy and is rapidly embracing novel techniques and strategies to help overcome the limitations of conventional approaches. Commercial molecular assays increase the applicability of PCR testing and may identify markers of antifungal resistance, which are of great clinical concern. Lateral flow assays simplify testing and turn-around time, with potential for point of care testing, while proximity ligation assays embrace the sensitivity of molecular testing with the specificity of antibody detection. The first evidence of patient risk stratification is being described and together with the era of next generation sequencing represents an exciting time in mycology.

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