scholarly journals Suppression Of Hepatitis C Virus Core Protein By Short Hairpin Rna Expression Vectors In The Core Protein Expression Huh-7 Cells

2007 ◽  
Vol 26 (6-7) ◽  
pp. 815-820 ◽  
Author(s):  
Hitoshi Suzuki ◽  
Hiroyasu Kaneko ◽  
Nobushige Tamai ◽  
Kunitada Shimotohno ◽  
Naoko Miyano-Kurosaki ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Protein Expression ◽  
Core Protein ◽  
Expression Vectors ◽  
Rna Expression ◽  
Hairpin Rna ◽  
The Core ◽  
Virus Core ◽  
Short Hairpin

scholarly journals Proteasomal Turnover of Hepatitis C Virus Core Protein Is Regulated by Two Distinct Mechanisms: a Ubiquitin-Dependent Mechanism and a Ubiquitin-Independent but PA28γ-Dependent Mechanism

2008 ◽  
Vol 83 (5) ◽  
pp. 2389-2392 ◽  
Author(s):  
Ryosuke Suzuki ◽  
Kohji Moriishi ◽  
Kouichirou Fukuda ◽  
Masayuki Shirakura ◽  
Koji Ishii ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Core Protein ◽  
Loss Of Function ◽  
Proteasome Activator ◽  
Binding Partner ◽  
The Core ◽  
Virus Core ◽  
Lysine Residues ◽  
Dependent Mechanism

ABSTRACT We have previously reported on the ubiquitylation and degradation of hepatitis C virus core protein. Here we demonstrate that proteasomal degradation of the core protein is mediated by two distinct mechanisms. One leads to polyubiquitylation, in which lysine residues in the N-terminal region are preferential ubiquitylation sites. The other is independent of the presence of ubiquitin. Gain- and loss-of-function analyses using lysineless mutants substantiate the hypothesis that the proteasome activator PA28γ, a binding partner of the core, is involved in the ubiquitin-independent degradation of the core protein. Our results suggest that turnover of this multifunctional viral protein can be tightly controlled via dual ubiquitin-dependent and -independent proteasomal pathways.

scholarly journals Suppression of hepatitis C virus replication by baculovirus vector-mediated short-hairpin RNA expression

FEBS Letters ◽  
2008 ◽  
Vol 582 (20) ◽  
pp. 3085-3089 ◽  
Author(s):  
Hitoshi Suzuki ◽  
Nobushige Tamai ◽  
Yuichiro Habu ◽  
Myint O.O. Chang ◽  
Hiroshi Takaku
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Replication ◽  
Short Hairpin Rna ◽  
Rna Expression ◽  
Hairpin Rna ◽  
Baculovirus Vector ◽  
Short Hairpin

Biosynthesis and biochemical properties of the hepatitis C virus core protein.

1994 ◽  
Vol 68 (6) ◽  
pp. 3631-3641 ◽  
Author(s):  
E Santolini ◽  
G Migliaccio ◽  
N La Monica
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Core Protein ◽  
Biochemical Properties ◽  
Virus Core

Truncated hepatitis C virus core protein encoded in hepatocellular carcinomas

2004 ◽  
Author(s):  
Rin Yamaguchi ◽  
Seiya Momosaki ◽  
Guang Gao ◽  
Chu Hsia ◽  
Masamichi Kojiro ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Core Protein ◽  
Virus Core ◽  
Hepatocellular Carcinomas

Genetic control of the murine humoral response to distinct epitopes of hepatitis C virus core protein

1995 ◽  
Vol 2 (1) ◽  
pp. 9-17 ◽  
Author(s):  
Z. Chen ◽  
I. Berkower ◽  
R. Y.-H. Wang ◽  
W.-M. Ching ◽  
H. J. Alter ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Genetic Control ◽  
Core Protein ◽  
Humoral Response ◽  
Virus Core
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