Inter-Individual Variation in Levels of the Detoxifying Enzyme Glutathione S-Transferase pi (GSTP1) in Placentas from a Mexican Population

Background: This study determined the effect of Biochanin A (BCA) on isoproterenol (ISO) induced Myocardial Infarction (MI) in male Wistar rats. Methods: Animals (weighing 150-180 g) were divided into four groups, with six animals in each group and pretreated with BCA (10mg/kg Body Weight [BW]) and ɑ-tocopherol (60mg/kg BW) for 30 days; and ISO (20mg/kg BW) was administrated subcutaneously on the 31st and 32nd day. Results: ISO-induced MI rats demonstrated the significant elevation of serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase, lactate dehydrogenase, creatine kinase-MB and cardiac troponin; however, concomitant pretreatment with BCA protected the rats from cardiotoxicity caused by ISO. Activities of antioxidant enzymes, such as superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase and glutathione reductase significantly reduced in the heart with ISO-induced MI. Pretreatment with BCA produced a marked reversal of these antioxidant enzymes related to MI-induced by ISO. Conclusion: In conclusion, this study suggested that BCA exerts cardioprotective effects through modulating lipid peroxidation, enhancing antioxidants, and detoxifying enzyme systems.

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Bemisia tabaci (Hemiptera: Aleyrodidae) Insecticide Resistance in Shandong Province, China

Journal of Economic Entomology ◽

10.1093/jee/toz315 ◽

2019 ◽

Vol 113 (2) ◽

pp. 911-917

Author(s):

Fan Wang ◽

Jin Liu ◽

Peng Chen ◽

Hong-Yang Li ◽

Jing-Jing Ma ◽

...

Keyword(s):

Insecticide Resistance ◽

Bemisia Tabaci ◽

Eastern China ◽

Ornamental Plants ◽

Shandong Province ◽

Transferase Activity ◽

Severe Damage ◽

Glutathione S Transferase ◽

Detoxifying Enzyme ◽

Moderate Resistance

Abstract The Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) complex comprises important pests and virus vectors in agricultural crops worldwide. In China, B. tabaci has spread to more than 20 provinces and caused severe losses of vegetables, fruits, and ornamental plants. However, B. tabaci has developed resistance to many insecticidal classes in Shandong Province, eastern China. In this study, we investigated the cryptic species, insecticide resistance and detoxifying enzymes of B. tabaci from six representative locations exhibiting severe damage in Shandong. At four of the six locations, B. tabaci Mediterranean (MED) comprised 100% of the samples collected. In a further two locations, species composition was predominantly (>94%) MED with B. tabaci Middle East–Asia Minor 1 (MEAM1), comprising a low proportion (<6%) of the samples collected. For all field populations, avermectin was the most effective insecticide against adult B. tabaci, pyriproxyfen had a significant effect on B. tabaci eggs and field populations were susceptible to pymetrozine. Six field populations of B. tabaci have developed low-to-moderate resistance to neonicotinoids. The detoxifying enzyme activity of carboxylesterase, glutathione S-transferase, and multifunctional oxidase were quantified. Multifunctional oxidase and glutathione S-transferase activity were positively correlated with insecticide resistance in several B. tabaci populations.

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Immunohistochemical expression of glutathione S-transferase placental type (GST-π), a detoxifying enzyme, in normal arachnoid villi and meningiomas

Virchows Archiv A Pathological Anatomy and Histopathology ◽

10.1007/bf01625729 ◽

1990 ◽

Vol 417 (6) ◽

pp. 493-496 ◽

Cited By ~ 1

Author(s):

A. Hara ◽

H. Yamada ◽

N. Sakai ◽

H. Hirayama ◽

T. Tanaka ◽

...

Keyword(s):

Immunohistochemical Expression ◽

Glutathione S Transferase ◽

Arachnoid Villi ◽

Detoxifying Enzyme

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Structures of herbicides in complex with their detoxifying enzyme glutathione S-transferase – explanations for the selectivity of the enzyme in plants

Structure ◽

10.1016/s0969-2126(98)00143-9 ◽

1998 ◽

Vol 6 (11) ◽

pp. 1445-1452 ◽

Cited By ~ 53

Author(s):

Lars Prade ◽

Robert Huber ◽

Babara Bieseler

Keyword(s):

Glutathione S Transferase ◽

Detoxifying Enzyme

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Effects of imidacloprid on detoxifying enzyme glutathione S-transferase on Folsomia candida (Collembola)

Environmental Science and Pollution Research ◽

10.1007/s11356-016-6686-1 ◽

2016 ◽

Vol 24 (12) ◽

pp. 11111-11119 ◽

Cited By ~ 11

Author(s):

Panwad Sillapawattana ◽

Andreas Schäffer

Keyword(s):

Folsomia Candida ◽

Glutathione S Transferase ◽

Detoxifying Enzyme

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Protective Responses Induced by Chiral 3-Dichloroacetyl Oxazolidine Safeners in Maize (Zea mays L.) and the Detoxification Mechanism

Molecules ◽

10.3390/molecules24173060 ◽

2019 ◽

Vol 24 (17) ◽

pp. 3060 ◽

Cited By ~ 4

Author(s):

Gao ◽

Liu ◽

Jiang ◽

Fu ◽

Zhao ◽

...

Keyword(s):

Acetolactate Synthase ◽

Antioxidant Enzyme Activities ◽

Optical Isomers ◽

Maize Seedlings ◽

Glutathione S Transferase ◽

Detoxifying Enzymes ◽

Detoxification Mechanism ◽

Detoxifying Enzyme ◽

Herbicide Safeners

Herbicide safeners selectively protect crops from herbicide injury while maintaining the herbicidal effect on the target weed. To some extent, the detoxification of herbicides is related to the effect of herbicide safeners on the level and activity of herbicide target enzymes. In this work, the expression of the detoxifying enzyme glutathione S-transferase (GST) and antioxidant enzyme activities in maize seedlings were studied in the presence of three potential herbicide safeners: 3-dichloroacetyl oxazolidine and its two optical isomers. Further, the protective effect of chiral herbicide safeners on detoxifying chlorsulfuron in maize was evaluated. All safeners increased the expression levels of herbicide detoxifying enzymes, including GST, catalase (CAT), and peroxidase (POD) to reduce sulfonylurea herbicide phytotoxicity in maize seedlings. Our results indicate that the R-isomer of 3-(dichloroacetyl)-2,2,5-trimethyl-1,3-oxazolidine can induce glutathione (GSH) production, GST activity, and the ability of GST to react with the substrate 1-chloro-2,4-dinitrobenzene (CDNB) in maize, meaning that the R-isomer can protect maize from damage by chlorsulfuron. Information about antioxidative enzyme activity was obtained to determine the role of chiral safeners in overcoming the oxidative stress in maize attributed to herbicides. The interaction of safeners and active target sites of acetolactate synthase (ALS) was demonstrated by molecular docking modeling, which indicated that both isomers could form a good interaction with ALS. Our findings suggest that the detoxification mechanism of chiral safeners might involve the induction of the activity of herbicide detoxifying enzymes as well as the completion of the target active site between the safener and chlorsulfuron.

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Detoxifying enzyme studies on cotton leafhopper, Amrasca biguttula biguttula (Ishida), resistance to neonicotinoid insecticides in field populations in Karnataka, India

Journal of Plant Protection Research ◽

10.1515/jppr-2016-0047 ◽

2016 ◽

Vol 56 (4) ◽

pp. 346-352 ◽

Cited By ~ 2

Author(s):

Banakar Halappa ◽

Rajashekar K. Patil

Keyword(s):

Activity Ratio ◽

Insect Pest ◽

Glutathione S Transferase ◽

Amrasca Biguttula Biguttula ◽

Detoxifying Enzyme ◽

Pesticide Usage ◽

In Situ Bioassay ◽

Very High

AbstractThe cotton leafhopper (Amrasca biguttula biguttula Ishida) is considered to be an alarming insect pest causing both quantitative and qualitative loss in cotton. In situ bioassay studies were done and the role of detoxifying enzymes in conferring resistance to neonicotinoid groups of insecticides in low (MUD), medium (DVG), high (HVR) and very high (GLB) pesticide usage areas of Karnataka were determined. Bioassay studies showed that imidacloprid, thiamethoxam, acetamiprid, thiacloprid and clothianidin registered varying levels of resistance for all the locations studied. The resistance ratio was high in imidacloprid (3.35, 8.57, 9.15 and 12.27 fold respectively) and the lowest in dinoferuran (1.86, 5.13, 6.71 and 9.88 fold respectively). Furthermore, the enzyme activity ratio (glutathione-S-transferase) was relatively greater, and corresponded to the higher LC50values of neonicotinoids for very high, high, medium and low pesticide usage areas. Our study suggested that the higher activity of the detoxifying enzyme in the resistance population of cotton leafhopper apparently has a significant role in endowing resistance to neonicotinoid groups of insecticides. However, this study recommends using neonicotinoids in cotton growing areas with caution.

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Participation of liver stem cells in cholangiocarcinogenesis after aflatoxin B1 exposure of glutathione S-transferase A3 knockout mice

Tumor Biology ◽

10.1177/1010428318777344 ◽

2018 ◽

Vol 40 (5) ◽

pp. 101042831877734 ◽

Cited By ~ 5

Author(s):

Zoran Ilic ◽

Tapan K Mondal ◽

Ian Guest ◽

Dana R Crawford ◽

Stewart Sell

Keyword(s):

Stem Cells ◽

Liver Cancer ◽

Knockout Mice ◽

Oval Cells ◽

Glutathione S Transferase ◽

Adult Mice ◽

Hepatocellular Carcinomas ◽

Liver Stem Cells ◽

Detoxifying Enzyme ◽

Aflatoxin B

Aflatoxin B1, arguably the most potent human carcinogen, induces liver cancer in humans, rats, trout, ducks, and so on, but adult mice are totally resistant. This resistance is because of a detoxifying enzyme, mouse glutathione S-transferase A3, which binds to and inactivates aflatoxin B1 epoxide, preventing the epoxide from binding to DNA and causing mutations. Glutathione S-transferase A3 or its analog has not been detected in any of the sensitive species, including humans. The generation of a glutathione S-transferase A3 knockout (represented as KO or -/-) mice has allowed us to study the induction of liver cancer in mice by aflatoxin B1. In contrast to the induction of hepatocellular carcinomas in other species, aflatoxin B1 induces cholangiocarcinomas in GSTA3-/- mice. In other species and in knockout mice, the induction of liver cancer is preceded by extensive proliferation of small oval cells, providing additional evidence that oval cells are bipolar stem cells and may give rise to either hepatocellular carcinoma or cholangiocarcinoma depending on the nature of the hepatocarcinogen and the species of animal. The recent development of mouse oval cell lines in our laboratory from aflatoxin B1-treated GSTA3-/- mice should provide a new venue for study of the properties and potential of putative mouse liver stem cells.

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