Natural dye baicalin was applied to dye silk and nylon 6 fibers. The effects of dyeing temperature, time, pH and dosages of sodium chloride and baicalin on dyeing properties of silk and nylon 6 fibers were analyzed, the color fastness of dyed fabrics also investigated. It was found that, the optimal process conditions were as follows: dyeing at 90oC(silk) or 80oC(Nylon 6), with pH 4, for about 60min. The built-up properties of baicalin on silk and nylon 6 fabrics were good, the color fastness of dyed fabrics were above 3-4 grade.