scholarly journals Identification of HnRNPC as a novel Tau exon 10 splicing factor using RNA antisense purification mass spectrometry

RNA Biology ◽  
2021 ◽  
pp. 1-13
Author(s):  
Sansi Xing ◽  
Jane Wang ◽  
Ruilin Wu ◽  
Marco M. Hefti ◽  
John F. Crary ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Splicing Factor ◽  
Exon 10

P1-037: PP1 Regulates the Alternative Splicing of TAU Exon 10 Through Splicing Factor SC35

2016 ◽  
Vol 12 ◽  
pp. P415-P415
Author(s):  
Wei Qian ◽  
Weihua Wang ◽  
Huanliang Liu ◽  
Jia Wang ◽  
Fei Liu
Keyword(s):  
Alternative Splicing ◽  
Splicing Factor ◽  
Exon 10

[P4-114]: SIRT1 ACTS ON SPLICING FACTOR SC35 TO REGULATE THE ALTERNATIVE SPLICING OF TAU EXON 10

2017 ◽  
Vol 13 (7S_Part_27) ◽  
pp. P1300-P1301
Author(s):  
Wei Qian ◽  
Xiaomin Yin ◽  
Xiaosu Jiang ◽  
Shuo Qian ◽  
Fei Liu
Keyword(s):  
Alternative Splicing ◽  
Splicing Factor ◽  
Exon 10

P1-351: Splicing factor SC35 regulates the alternative splicing of tau exon 10

2010 ◽  
Vol 6 ◽  
pp. S275-S275
Author(s):  
Wei Qian ◽  
Hongwei Liang ◽  
Jianhua Shi ◽  
Jianlan Gu ◽  
Xiaolin Wu ◽  
...  
Keyword(s):  
Alternative Splicing ◽  
Splicing Factor ◽  
Exon 10

scholarly journals A termination-independent role of Rat1 in cotranscriptional splicing

2020 ◽  
Author(s):  
Zuzer Dhoondia ◽  
Hesham Elewa ◽  
Marva Malik ◽  
Zahidur Arif ◽  
Roger Pique-Regi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Elongation Rate ◽  
Mrna Splicing ◽  
Splicing Factor ◽  
Splicing Factors ◽  
Human Homolog ◽  
Termination Factor ◽  
Yeast Genes ◽  
Nuclear Processes

AbstractThe yeast termination factor Rat1, and its human homolog Xrn2, have been implicated in multiple nuclear processes. Here we report a novel role of Rat1 in mRNA splicing. Rat1 mutants display increased levels of unspliced transcripts. Accumulation of unspliced transcripts was not due to a failure to degrade unspliced mRNA, disruption of termination or an increased elongation rate in Rat1 mutants. ChIP-Seq analysis revealed Rat1 crosslinking to the introns of a subset of yeast genes. Mass spectrometry and coimmunoprecipitation revealed interaction of Rat1 with the Clf1, Isy1, Yju2, Prp43, and Sub2 splicing factors. Furthermore, recruitment of the Prp2 splicing factor on the intron was compromised in the Rat1 mutant. Based on these findings we propose that Rat1 has a novel role in splicing of a subset of mRNA in budding yeast.

How SIMS microscopy can be used in medicine

1992 ◽  
Vol 50 (2) ◽  
pp. 1602-1603
Author(s):  
Philippe Fragu
Keyword(s):  
Mass Spectrometry ◽  
Biomedical Applications ◽  
Secondary Ion Mass Spectrometry ◽  
Chemical Elements ◽  
Biological Applications ◽  
The Past ◽  
Potential Source ◽  
Secondary Ion ◽  
Chemical Integrity ◽  
Scientific Endeavour

The identification, localization and quantification of intracellular chemical elements is an area of scientific endeavour which has not ceased to develop over the past 30 years. Secondary Ion Mass Spectrometry (SIMS) microscopy is widely used for elemental localization problems in geochemistry, metallurgy and electronics. Although the first commercial instruments were available in 1968, biological applications have been gradual as investigators have systematically examined the potential source of artefacts inherent in the method and sought to develop strategies for the analysis of soft biological material with a lateral resolution equivalent to that of the light microscope. In 1992, the prospects offered by this technique are even more encouraging as prototypes of new ion probes appear capable of achieving the ultimate goal, namely the quantitative analysis of micron and submicron regions. The purpose of this review is to underline the requirements for biomedical applications of SIMS microscopy.Sample preparation methodology should preserve both the structural and the chemical integrity of the tissue.

Imaging of Al-Li-Cu alloys with a Scanning Ion Microprobe

1990 ◽  
Vol 48 (2) ◽  
pp. 314-315
Author(s):  
K.K. Soni ◽  
D.B. Williams ◽  
J.M. Chabala ◽  
R. Levi-Setti ◽  
D.E. Newbury
Keyword(s):  
Mass Spectrometry ◽  
Secondary Ion Mass Spectrometry ◽  
Equilibrium Phase ◽  
Icosahedral Symmetry ◽  
Ion Microprobe ◽  
X Ray ◽  
Cu Alloys ◽  
Two Phases ◽  
The University ◽  
Secondary Ion

In contrast to the inability of x-ray microanalysis to detect Li, secondary ion mass spectrometry (SIMS) generates a very strong Li+ signal. The latter’s potential was recently exploited by Williams et al. in the study of binary Al-Li alloys. The present study of Al-Li-Cu was done using the high resolution scanning ion microprobe (SIM) at the University of Chicago (UC). The UC SIM employs a 40 keV, ∼70 nm diameter Ga+ probe extracted from a liquid Ga source, which is scanned over areas smaller than 160×160 μm2 using a 512×512 raster. During this experiment, the sample was held at 2 × 10-8 torr.In the Al-Li-Cu system, two phases of major importance are T1 and T2, with nominal compositions of Al2LiCu and Al6Li3Cu respectively. In commercial alloys, T1 develops a plate-like structure with a thickness <∼2 nm and is therefore inaccessible to conventional microanalytical techniques. T2 is the equilibrium phase with apparent icosahedral symmetry and its presence is undesirable in industrial alloys.

Applications of Time-OF-Flight Secondary Ion Mass Spectrometry (TOF-SIMS)

1990 ◽  
Vol 48 (2) ◽  
pp. 308-309
Author(s):  
Bruno Schueler ◽  
Robert W. Odom
Keyword(s):  
Mass Spectrometry ◽  
Secondary Ion Mass Spectrometry ◽  
Structural Information ◽  
Time Of Flight ◽  
Biological Tissues ◽  
Polymer Surfaces ◽  
Tof Sims ◽  
Secondary Ions ◽  
Ion Mass Spectrometry ◽  
Secondary Ion

Time-of-flight secondary ion mass spectrometry (TOF-SIMS) provides unique capabilities for elemental and molecular compositional analysis of a wide variety of surfaces. This relatively new technique is finding increasing applications in analyses concerned with determining the chemical composition of various polymer surfaces, identifying the composition of organic and inorganic residues on surfaces and the localization of molecular or structurally significant secondary ions signals from biological tissues. TOF-SIMS analyses are typically performed under low primary ion dose (static SIMS) conditions and hence the secondary ions formed often contain significant structural information.This paper will present an overview of current TOF-SIMS instrumentation with particular emphasis on the stigmatic imaging ion microscope developed in the authors’ laboratory. This discussion will be followed by a presentation of several useful applications of the technique for the characterization of polymer surfaces and biological tissues specimens. Particular attention in these applications will focus on how the analytical problem impacts the performance requirements of the mass spectrometer and vice-versa.

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