Arthur Almeida Rodrigues
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Sebastião Carvalho Vasconcelos Filho
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Caroline Müller
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Douglas Almeida Rodrigues
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Juliana de Fátima Sales
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Native Cerrado plants are exposed to soils with low pH and high availability of Al. In this study, we measured the Al content in adult plants, and investigated the effects of various Al doses on germination and early development of Eugenia dysenterica plants. For germination tests, the seeds were soaked in Al solution and evaluated for twenty days in growth chambers. In a second experiment, young plants were cultivated in hydroponic systems with various Al concentrations to evaluate the morphological, anatomical and physiological characteristics of E. dysenterica. Anatomical changes and low germinative vigor were observed in seeds germinated in 600 and 800 μmol Al3+ L−1. In the hydroponic system, 200 μmol Al3+ L−1 stimulated root growth in young plants. The activity of antioxidant enzymes and the accumulation of phenolic compounds were greatest at the highest Al doses, preventing changes in gas exchange and chlorophyll a fluorescence. Starch grain accumulation was noted in plant cells exposed to 200 and 400 μmol Al3+ L−1. Adult E. dysenterica trees also accumulated Al in leaves, bark and seeds. These data suggest that E. dysenterica is tolerant to Al.
Starch grain extraction in lime-plastered archaeological floors
