scholarly journals An update clinical application of amniotic fluid-derived stem cells (AFSCs) in cancer cell therapy and tissue engineering

2016 ◽  
Vol 45 (4) ◽  
pp. 765-774 ◽  
Author(s):  
Shiva Gholizadeh-Ghaleh Aziz ◽  
Ezzatollah Fathi ◽  
Mohammad Rahmati-Yamchi ◽  
Abolfazl Akbarzadeh ◽  
Zahra Fardyazar ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Cell Therapy ◽  
Amniotic Fluid ◽  
Clinical Application ◽  
Cancer Cell

298 DIFFERENTIATION OF CANINE AMNIOTIC FLUID MESENCHYMAL STEM CELLS INTO NEURONAL PRECURSORS

2011 ◽  
Vol 23 (1) ◽  
pp. 246
Author(s):  
E. Y. Kim ◽  
S. A. Choi ◽  
J. H. Lee ◽  
K. J. Kim ◽  
K. S. Park ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Therapy ◽  
Amniotic Fluid ◽  
Neural Precursor ◽  
Mrna Levels ◽  
Before And After ◽  
Induction Of Differentiation

The amniotic fluids contain mesenchymal stem cells and can be readily available for tissue engineering. Recently, regenerative treatments such as tissue engineering, cell therapy, and transplantation have shown potential in clinical trials of degenerative diseases. Physiologically, disease presentation and clinical responses in the dog are much more similar to that in the human compared with other traditional mammalian models. In addition, several researchers have demonstrated Canis familiaris is a suitable model for human diseases. The aim of the present study was to investigate whether canine amniotic fluid (cAF)-derived mesenchymal stem cells (MSC) can differentiate into neural precursor cells in vitro by neural induction reagent. The conditions of differentiation of MSC into neural cells were DMEM and N2-supplement, dibutyryl cyclic adenosine monophosphate, and butylated hydroxyanisole. During neural precursor differentiation, cAF-MSC can progressively acquire neuron-like morphology. Expressions of neuron cell-specific markers were examined before and after in vitro induction of differentiation. Changes in mRNA levels of specific genes were quantified by RT-PCR. The mRNA levels of NEFL (730%), GFAP (350%), β-tubuline 3 (2900%), and NSE (960%) were significantly increased after induction. The value of change in mRNA levels before and after induction was evaluated with the Image J program. In addition, the nestin, β-tubuline 3, and tyrosine hydroxylase protein expressions were confirmed by immunocytochemistry assay following the induction of differentiation, compared with the noninduction. In conclusion, this study demonstrated that cAF-MSC have great potential for neural precursor differentiation in vitro. Therefore, amniotic fluid may be a suitable alternative source of stem cells and can be applied to cell therapy in neurodegeneration diseases including Parkinson’s disease, Alzheimer’s disease, and Huntington’s disease.

scholarly journals Amniotic Fluid-Derived Stem Cells (AFSC) and Their Application in Cell Therapy and Tissue Engineering

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Syeda Zahra Anum ◽  
Seyed Raheel Muzavir ◽  
Ahmad Hassan ◽  
Amir Ali Khan ◽  
Aftab Ahmad
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Cell Therapy ◽  
Amniotic Fluid

Uncovering the Diversification of Tissue Engineering on the Emergent Areas of Stem Cells, Nanotechnology and Biomaterials

2020 ◽  
Vol 15 (3) ◽  
pp. 187-201 ◽  
Author(s):  
Sunil K. Dubey ◽  
Amit Alexander ◽  
Munnangi Sivaram ◽  
Mukta Agrawal ◽  
Gautam Singhvi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Immune System ◽  
Clinical Application ◽  
Cell Types ◽  
Patient Specific ◽  
Potential Strategy ◽  
Induced Pluripotent ◽  
Treat All ◽  
The Impact

Damaged or disabled tissue is life-threatening due to the lack of proper treatment. Many conventional transplantation methods like autograft, iso-graft and allograft are in existence for ages, but they are not sufficient to treat all types of tissue or organ damages. Stem cells, with their unique capabilities like self-renewal and differentiate into various cell types, can be a potential strategy for tissue regeneration. However, the challenges like reproducibility, uncontrolled propagation and differentiation, isolation of specific kinds of cell and tumorigenic nature made these stem cells away from clinical application. Today, various types of stem cells like embryonic, fetal or gestational tissue, mesenchymal and induced-pluripotent stem cells are under investigation for their clinical application. Tissue engineering helps in configuring the stem cells to develop into a desired viable tissue, to use them clinically as a substitute for the conventional method. The use of stem cell-derived Extracellular Vesicles (EVs) is being studied to replace the stem cells, which decreases the immunological complications associated with the direct administration of stem cells. Tissue engineering also investigates various biomaterials to use clinically, either to replace the bones or as a scaffold to support the growth of stemcells/ tissue. Depending upon the need, there are various biomaterials like bio-ceramics, natural and synthetic biodegradable polymers to support replacement or regeneration of tissue. Like the other fields of science, tissue engineering is also incorporating the nanotechnology to develop nano-scaffolds to provide and support the growth of stem cells with an environment mimicking the Extracellular matrix (ECM) of the desired tissue. Tissue engineering is also used in the modulation of the immune system by using patient-specific Mesenchymal Stem Cells (MSCs) and by modifying the physical features of scaffolds that may provoke the immune system. This review describes the use of various stem cells, biomaterials and the impact of nanotechnology in regenerative medicine.

scholarly journals Dental Pulp Stem Cells and Tissue Engineering Strategies for Clinical Application on Odontoiatric Field

10.5772/24871 ◽  
2011 ◽  
Author(s):  
Zavan Barbara ◽  
Bressan Eriberto ◽  
Sivolella Stefano ◽  
Brunello Giulia ◽  
Gardin Chiara ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Clinical Application ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells

C29 POTENTIAL OF AMNIOTIC FLUID-DERIVED STEM CELLS FOR UROLOGICAL TISSUE ENGINEERING

2011 ◽  
Vol 10 (9) ◽  
pp. 620
Author(s):  
J. Olkowska ◽  
N. Gurtowska ◽  
A. Bajek ◽  
M. Walentowicz-Sadlecka ◽  
P. Sadlecki ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Amniotic Fluid

Review of the 3rd IPLASS meeting: toward clinical applications of placental and endometrial stem cells

2014 ◽  
Vol 2 (2) ◽  
pp. 161-162
Author(s):  
G. Lobyntseva
Keyword(s):  
United States ◽  
Stem Cells ◽  
Stem Cell ◽  
Cell Therapy ◽  
Clinical Application ◽  
Clinical Applications ◽  
Endometrial Stem Cells ◽  
Poster Presentations ◽  
Scientific Value

From 10 to 12 September the 3rd IPLASS Meeting: toward clinical application of endometrial and placental stem cells took place in Granada (Spain). The organizer of the symposium was the International Placenta Stem Cell Society (IPLASS) headed by President Dr. Ornella Parolini (Italy). There were presented 29 oral reports and 25 poster presentations. Scientific and clinical institutions in Italy, United States, Australia, Austria, Netherlands, Sweden, Israel, India, Portugal, Spain, France, Argentina, Brazil, Japan, China, Chile, and Germany are engaged in research of characteristics of stem cells derived from the placenta. Scientists from Ukraine (Institute of Cell Therapy, Kyiv) presented three reports, which scientific value was awarded with a diploma and a prize.

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