Taraxasterol from Taraxacum prevents concanavalin A-induced acute hepatic injury in mice via modulating TLRs/NF-κB and Bax/Bc1-2 signalling pathways

Pharmacologic Relevance.Resveratrol, an antioxidant derived from grapes, has been reported to modulate the inflammatory process. In this study, we investigated the effects of resveratrol and its mechanism of protection on concanavalin-A- (ConA-) induced liver injury in mice.Materials and Methods.Acute autoimmune hepatitis was induced by ConA (20 mg/kg) in Balb/C mice; mice were treated with resveratrol (10, 20, and 30 mg/kg) daily by oral gavage for fourteen days prior to a single intravenous injection of ConA. Eight hours after injection, histologic grading, proinflammatory cytokine levels, and hedgehog pathway activity were determined.Results.After ConA injection, the cytokines IL-2, IL-6, and TNF-αwere increased, and Sonic hedgehog (Shh), Glioblastoma- (Gli-) 1, and Patched (Ptc) levels significantly increased. Pretreatment with resveratrol ameliorated the pathologic effects of ConA-induced autoimmune hepatitis and significantly inhibited IL-2, IL-6, TNF-α, Shh, Gli-1, and Ptc. The effects of resveratrol on the hedgehog pathway were studied by western blotting and immunohistochemistry. Resveratrol decreased Shh expression, possibly by inhibiting Shh expression in order to reduce Gli-1 and Ptc expression.Conclusion.Resveratrol protects against ConA-induced autoimmune hepatitis by decreasing cytokines expression in mice. The decreases seen in Gli-1 and Ptc may correlate with the amelioration of hedgehog pathway activity.

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Hepatoprotective Activity of Nigella sativa and Piper nigrum against Concanavalin A-Induced Acute Liver Injury in Mouse Model

Pakistan Veterinary Journal ◽

10.29261/pakvetj/2020.076 ◽

2021 ◽

Vol 41 (01) ◽

pp. 78-84

Author(s):

Aqsa Mushtaq

Keyword(s):

Liver Injury ◽

Concanavalin A ◽

Biochemical Parameters ◽

Hepatic Injury ◽

Therapeutic Potential ◽

Nigella Sativa ◽

Transaminase Level ◽

Piper Nigrum ◽

Phytochemical Analysis ◽

Acute Hepatic Injury

Nigella sativa and Piper nigrum are implicated in the treatment of various disorders, especially in the management of metabolic, infectious and hepatorenal ailments. However, data on the mechanism behind therapeutic potential of N. sativa and P. nigrum in liver diseases is scarce. The present study investigated the hepatoprotective potential of 70% methanolic extract of N. sativa (NSE) and P. nigrum (PNE) at varying dose levels (100 to 400 mg/kg body weight) against concanavalin A (conA)-induced liver injury. Qualitative phytochemical analysis of plant extracts was performed. Acute hepatic injury was induced by administering intraperitoneally 12mg/kg conA in Balb/c mice. The extent of hepatic injury was measured by analysing serum biochemical parameters, liver antioxidant stress assay and histopathology. Data were analysed statistically. NSE and PNE showed dosedependent hepatoprotective efficacy by lowering the conA-dependent rise in liver transaminase level. Treatment with NSE (400 mg/Kg) and PNE (400 mg/Kg) ameliorated conA-induced alterations in serum oxidative stress markers, biochemical parameters, liver function markers and histopathology. NSE indicated greater effectiveness to ameliorate the acute hepatic injury in comparison with PNE at the same dose. Collectively, pretreatment with NSE and PNE attenuated the liver injury induced by conA, might be through alleviating the antioxidant capacity of experimental mice

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Arbutin attenuates ethanol‐induced acute hepatic injury by the modulation of oxidative stress and Nrf‐2/HO‐1 signaling pathway

Journal of Biochemical and Molecular Toxicology ◽

10.1002/jbt.22872 ◽

2021 ◽

Author(s):

Rongsheng Wang ◽

Jinji Mu

Keyword(s):

Oxidative Stress ◽

Signaling Pathway ◽

Hepatic Injury ◽

Acute Hepatic Injury

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Acute liver injury associated with Oxyfluorfen toxicity

SAGE Open Medical Case Reports ◽

10.1177/2050313x211000454 ◽

2021 ◽

Vol 9 ◽

pp. 2050313X2110004

Author(s):

Selladurai Pirasath ◽

Ayshanie Gayanthika Samasundara Mudiyanselage ◽

Manosha Harshani Seneviratne

Keyword(s):

Sri Lanka ◽

Liver Injury ◽

Young Woman ◽

Broad Spectrum ◽

Hepatic Injury ◽

Acute Liver Injury ◽

Toxic Dose ◽

Acute Hepatic Injury ◽

Haem Biosynthesis ◽

Spectrum Control

Oxyfluorfen is a phenoxyphenyl-type herbicide which is used for broad-spectrum control of broadleaf and grassy weeds. Ingestion of toxic dose of oxyfluorfen can be fatal among animals. However, toxicity to humans are rare in literature. The alterations in haem biosynthesis (anaemia) and in liver are the primary toxic effects. There are no specific antidotes and none of the current treatments have proven efficacious till date. Therefore, prevention needs to be the utmost priority, and on exposure, aggressive decontamination should be initiated. Herein, we described an oxyfluorfen toxicity with acute hepatic injury in a young woman who presented with a deliberate self-harming with an oxyfluorfen poisoning in Sri Lanka.

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