scholarly journals Heparan sulfate proteoglycans from mouse mammary epithelial cells: localization on the cell surface with a monoclonal antibody.

1985 ◽  
Vol 101 (3) ◽  
pp. 976-984 ◽  
Author(s):  
M Jalkanen ◽  
H Nguyen ◽  
A Rapraeger ◽  
N Kurn ◽  
M Bernfield
Keyword(s):  
Monoclonal Antibody ◽  
Epithelial Cells ◽  
Cell Surface ◽  
Heparan Sulfate ◽  
Mammary Epithelial Cells ◽  
Polyacrylamide Gel Electrophoresis ◽  
Specific Antigen ◽  
Mammary Epithelial ◽  
Immunofluorescent Staining ◽  
Nmumg Cell

Mouse mammary epithelial cells, of the normal murine mammary gland (NMuMG) cell line, bear a heparan sulfate-rich proteoglycan (HSPG) on their surfaces. A hybridoma (281-2) secreting a monoclonal antibody that recognizes this HSPG was produced by fusion of SP-2/0 myeloma cells with spleen cells from rats immunized with NMuMG cells. The 281-2 monoclonal antibody is directed against the core protein of the cell surface HSPG, as demonstrated by (a) recognition of the isolated proteoglycan but not its glycosaminoglycan chains, (b) co-localization of 281-2-specific antigen and radioactive cell surface HSPG on gradient polyacrylamide gel electrophoresis and on isopycnic centrifugation, and (c) abolition of immunofluorescent staining of the NMuMG cell surface by the intact, but not the protease-digested ectodomain of the cell surface HSPG. The antibody is specific for cell surface HSPG and does not recognize the HSPG that accumulates extracellularly beneath the basal cell surface. Therefore, the 281-2 antibody may be used to isolate the cell surface HSPG and to explore its distribution in tissues.

Parathyroid hormone-related protein increases cAMP production in mammary epithelial cells

1993 ◽  
Vol 264 (3) ◽  
pp. E471-E475 ◽  
Author(s):  
S. Ferrari ◽  
R. Rizzoli ◽  
C. Chaponnier ◽  
G. Gabbiani ◽  
J. P. Bonjour
Keyword(s):  
Parathyroid Hormone ◽  
Epithelial Cells ◽  
Breast Tissue ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Immunofluorescent Staining ◽  
Regulatory Function ◽  
Related Protein ◽  
Camp Production ◽  
Parathyroid Hormone Related Protein

Parathyroid hormone-related protein (PTHrP) is a major cause of malignant hypercalcemia but has been found in many nontumoral tissues as well. Thus it is produced by the mammary gland during lactation and released into milk. Whether PTHrP directly affects breast tissue is however not known. We investigated the effects of PTHrP on adenosine 3',5'-cyclic monophosphate (cAMP) production in primary cultures of mammary epithelial cells isolated from lactating rats. On the 7th day in culture, synthetic PTHrP-(1-34), recombinant (r) PTHrP-(1-108), and rPTHrP-(1-141) stimulated cAMP production in a concentration-dependent manner. Thus PTHrP-(1-34) induced a 1.92 +/- 0.04-fold stimulation of cAMP production (mean +/- SE, n = 5 separate experiments, P < 0.001). At the time of maximal responsiveness to PTHrP, a significant proportion of the cells was characterized by an elongated shape and a positive immunofluorescent staining for both prekeratin and alpha-smooth muscle actin 1, compatible with a myoepithelial phenotype. It therefore appears that PTHrP can stimulate the production of cAMP in mammary cells, suggesting a possible autocrine/paracrine regulatory function for PTHrP in breast tissue.

scholarly journals Loss of the Heparan Sulfate Sulfotransferase, Ndst1, in Mammary Epithelial Cells Selectively Blocks Lobuloalveolar Development in Mice

PLoS ONE ◽  
2010 ◽  
Vol 5 (5) ◽  
pp. e10691 ◽  
Author(s):  
Brett E. Crawford ◽  
Omai B. Garner ◽  
Joseph R. Bishop ◽  
David Y. Zhang ◽  
Kevin T. Bush ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Heparan Sulfate ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Lobuloalveolar Development
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